A comprehensive study on the improvement of oxidation stability and NOx emission levels by antioxidant addition to biodiesel blends in a light-duty diesel engine

RSC Advances ◽  
2016 ◽  
Vol 6 (27) ◽  
pp. 22436-22446 ◽  
Author(s):  
M. M. Rashed ◽  
H. H. Masjuki ◽  
M. A. Kalam ◽  
Abdullah Alabdulkarem ◽  
H. K. Imdadul ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Diesel Engine ◽  
Unsaturated Fatty Acid ◽  
Moringa Oleifera ◽  
Fatty Acid Content ◽  
Oxidation Stability ◽  
High Unsaturated Fatty Acid ◽  
Light Duty ◽  
Biodiesel Feedstock ◽  
Comprehensive Study

Moringa oleifera oil, a non-edible biodiesel feedstock with high unsaturated fatty acid content, was used in this study.

scholarly journals Chromosome-Level Genome Assembly and HazelOmics Database Construction Provides Insights Into Unsaturated Fatty Acid Synthesis and Cold Resistance in Hazelnut (Corylus heterophylla)

2021 ◽  
Vol 12 ◽  
Author(s):  
Jianfeng Liu ◽  
Heng Wei ◽  
Xingzheng Zhang ◽  
Hongli He ◽  
Yunqing Cheng ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Genome Assembly ◽  
Unsaturated Fatty Acid ◽  
Northeast China ◽  
Fatty Acid Content ◽  
Cold Climate ◽  
Maturity Stage ◽  
High Unsaturated Fatty Acid ◽  
Genome Database ◽  
Chromosome Level

Corylus heterophylla (2n = 22) is the most widely distributed, unique, and economically important nut species in China. Chromosome-level genomes of C. avellana, C. heterophylla, and C. mandshurica have been published in 2021, but a satisfactory hazelnut genome database is absent. Northeast China is the main distribution and cultivation area of C. heterophylla, and the mechanism underlying the adaptation of C. heterophylla to extremely low temperature in this area remains unclear. Using single-molecule real-time sequencing and the chromosomal conformational capture (Hi-C) assisted genome assembly strategy, we obtained a high-quality chromosome-scale genome sequence of C. heterophylla, with a total length of 343 Mb and scaffold N50 of 32.88 Mb. A total of 94.72% of the test genes from the assembled genome could be aligned to the Embryophyta_odb9 database. In total, 22,319 protein-coding genes were predicted, and 21,056 (94.34%) were annotated in the assembled genome. A HazelOmics online database (HOD) containing the assembled genome, gene-coding sequences, protein sequences, and various types of annotation information was constructed. This database has a user-friendly and straightforward interface. In total, 439 contracted genes and 3,810 expanded genes were identified through genome evolution analysis, and 17 expanded genes were significantly enriched in the unsaturated fatty acid biosynthesis pathway (ko01040). Transcriptome analysis results showed that FAD (Cor0058010.1), SAD (Cor0141290.1), and KAT (Cor0122500.1) with high expression abundance were upregulated at the ovule maturity stage. We deduced that the expansion of these genes may promote high unsaturated fatty acid content in the kernels and improve the adaptability of C. heterophylla to the cold climate of Northeast China. The reference genome and database will be beneficial for future molecular breeding and gene function studies in this nut species, as well as for evolutionary research on species of the order Fagales.

scholarly journals Responses of Unsaturated Fatty Acid in Membrane Lipid and Antioxidant Enzymes to Chilling Stress in Sweet Sorghum (Sorghum bicolor (L.) Moench) Seedling

2016 ◽  
Vol 8 (9) ◽  
pp. 71 ◽  
Author(s):  
Yuanyuan Guo ◽  
Shanshan Liu ◽  
Zhen Yang ◽  
Shanshan Tian ◽  
Na Sui
Keyword(s):  
Antioxidant Enzymes ◽  
Fatty Acid ◽  
Sorghum Bicolor ◽  
Unsaturated Fatty Acid ◽  
Sweet Sorghum ◽  
Membrane Lipids ◽  
Chilling Stress ◽  
Fatty Acid Content ◽  
Membrane Lipid ◽  
Protective Enzyme

<p>Low temperature is a major factor limiting the productivity and geographical distribution of many plant species. In this study, we investigated the effect of chilling stress (10 <sup>o</sup>C) on seedling growth in two sweet sorghum (<em>Sorghum bicolor </em>(L.) Moench) inbred lines (M-81E and Roma). Results showed that the chilling resistance of M-81E was higher than that of Roma. The Fv/Fm in leaves of M-81E decreased less than that of Roma during chilling stress. After 24 h of chilling stress, the Fv/Fm of M-81E and Roma decreased by 24.3 and 45.8%, respectively. Fo was also affected significantly during chilling stress. Malondialdehyde (MDA), an indicator of lipid peroxidation caused by ROS, increased during chilling stress. The contents of MDA increased less in leaves of M-81E than that in Roma under chilling stress. The antioxidant enzymes (SOD and APX) activity of M-81E was higher than those of Roma during chilling stress. The unsaturated fatty acid content and the double bond index (DBI) of major membrane lipids of MGDG, DGDG, SQDG, PC, PE and PG of M-81E significantly increased after 24 h of chilling treatment (10 <sup>o</sup>C). The DBI of MGDG, DGDG, SQDG, PC and PG of Roma significantly decreased. These results showed that the chilling tolerance of M-81E was higher than that of Roma by increasing of unsaturated fatty acid in membrane lipid and powerful protective enzyme system at seedling stage.</p>

Anti-Oxidation Stability and its Mechanism of Waste Oil Biodiesel

2013 ◽  
Vol 739 ◽  
pp. 80-84
Author(s):  
Jiang Wu ◽  
Bo Shui Chen ◽  
Jian Hua Fang ◽  
Jiu Wang
Keyword(s):  
Fatty Acid ◽  
Double Bond ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Unsaturated Fatty Acid ◽  
Oxidation Stability ◽  
Acid Methyl Ester ◽  
Waste Oil ◽  
Acid Methyl ◽  
Set Up

The anti-oxidation stability of waste oil biodiesel (WME) was evaluated on an oxidation simulator set up by the author. The results showed that oxidative stability of WME was worse than that of petrodiesel by exhibiting higher acid values and peroxide values, as well as greater viscosity increases after oxidation. Furthermore, a conjecture was taken about the configurational changes and the oxidation mechanisms of unsaturated fatty acid methyl ester molecules in the oxidation process, according to the principles of free radical reactions and the results of both infrared and ultraviolet spectroscopic analysis. An idea was put forward that, during oxidation, cis-trans isomerization might occur in unsaturated fatty acid methyl ester molecules and conjugated double-bond might produce due to transfer of double-bond.

scholarly journals The biochemical content and antioxidant capacities of endemic Tanacetum densum (Lab.) Schultz Bip. subsp. laxum, and Tanacetum densum (Lab.) Schultz Bip. subsp. amani Heywood growing in Turkey

2021 ◽  
Vol 81 (4) ◽  
pp. 1106-1114
Author(s):  
İ. Emre
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Acid Content ◽  
Unsaturated Fatty Acids ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Fatty Acid Content ◽  
Radical Scavenging ◽  
Unsaturated Fatty Acid Content

Abstract Medicinal plants have a significant role in preventing and curing several diseases, and Tanacetum L. is one of these plants. The aim of the present study is to determine the fatty acid, lipid-soluble vitamin, sterol, phenolic content, and antioxidant capacity of Tanacetum densum subsp. laxum and Tanacetum densum subsp. amani, to compare the effect of altitude on the biochemical content and to compare systematically by using fatty acids and phenolics. This study showed that palmitic acid (C16:0) and stearic acid (C18:0) are major sources of saturated fatty acid and oleic acid (C18:1 n9), and linoleic acid (18:2 n6c) and a-linolenic acid (C18:3 n3) are the principal unsaturated fatty acids in the two endemic Tanacetum densum taxa. Also, this study found that the unsaturated fatty acid content (60.11±1.61%) of Tanacetum densum subsp. laxum was higher than the unsaturated fatty acid content (44.13±1.28%) of Tanacetum densum subsp. amani. And also, the ω6/ω3 ratio of Tanacetum densum subsp. laxum (1.74) and Tanacetum densum subsp. amani (1.60) was found to be similar. However, this study determined that the lipid soluble vitamin and sterol content of two endemic Tanacetum taxa are low except for stigmasterol. Present study showed that catechin is principal phenolic in the Tanacetum densum taxa. This study also found that Tanacetum densum subsp. laxum and Tanacetum densum subsp. amani had the highest levels of catechin, vanillic acid, and caffeic acid content though the phenolic amounts, particularly catechin and quercetin, were dissimilar in the T. densum taxa. This study suggested that ecological conditions such as altitude may affect the biochemical content of two endemic Tanacetum densum taxa. Furthermore, the current study determined that two endemic Tanacetum L. taxa had potent radical scavenging capacities and found a correlation between total phenolics and antioxidant activity.

scholarly journals The biosynthesis of triacylglycerols in microsomal preparations of developing cotyledons of sunflower (Helianthus annuus L.)

1984 ◽  
Vol 220 (2) ◽  
pp. 481-488 ◽  
Author(s):  
S Stymne ◽  
A K Stobart
Keyword(s):  
Fatty Acid ◽  
Helianthus Annuus ◽  
Unsaturated Fatty Acid ◽  
Fatty Acid Content ◽  
Acid Synthesis ◽  
Carthamus Tinctorius ◽  
Persea Americana ◽  
Acyl Exchange ◽  
The Relationship ◽  
Continuous Enrichment

The synthesis of triacylglycerols was investigated in microsomes (microsomal fractions) prepared from the developing cotyledons of sunflower (Helianthus annuus). Particular emphasis was placed on the mechanisms involved in controlling the C18- unsaturated-fatty-acid content of the oils. We have demonstrated that the microsomes were capable of: the transfer of oleate from acyl-CoA to position 2 of sn-phosphatidylcholine for its subsequent desaturation and the return of the polyunsaturated products to the acyl-CoA pool by further acyl exchange; the acylation of sn-glycerol 3-phosphate with acyl-CoA to yield phosphatidic acid, which was further utilized in diacyl- and tri-acylglycerol synthesis; and (3) the equilibrium of a diacylglycerol pool with phosphatidylcholine. The acyl exchange between acyl-CoA and position 2 of sn-phosphatidylcholine coupled to the equilibration of diacylglycerol and phosphatidylcholine brings about the continuous enrichment of the glycerol backbone with C18 polyunsaturated fatty acids for triacylglycerol production. Similar reactions were found to operate in another oilseed plant, safflower (Carthamus tinctorius L.). On the other hand, the microsomes of avocado (Persea americana) mesocarp, which synthesize triacylglycerol via the Kennedy [(1961) Fed. Proc. Fed. Am. Soc. Exp. Biol. 20, 934-940] pathway, were deficient in acyl exchange and the diacylglycerol in equilibrium phosphatidylcholine interconversion. The results provide a working model that helps to explain the relationship between C18- unsaturated-fatty-acid synthesis and triacylglycerol production in oilseeds.

scholarly journals Teosinte (Dioon mejiae) Flour: Nutritional and Physicochemical Characterization of the Seed Flour of the Living Fossil in Honduras

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 481
Author(s):  
José-Miguel Bastias-Montes ◽  
Laura-Elena Flores-Varela ◽  
Onán-Alonso Reyes-Calderón ◽  
Carla Vidal-San-Martín ◽  
Ociel Muñoz-Fariña ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Content ◽  
Physicochemical Characterization ◽  
Sodium Concentration ◽  
Essential Amino Acids ◽  
High Unsaturated Fatty Acid ◽  
Total Dietary Fiber ◽  
Living Fossil ◽  
Physicochemical Composition ◽  
Seed Flour

Teosinte (Dioon mejiae) is a dioecious tree native to Honduras, whose seeds are used to make flour for the preparation of traditional foods and beverages. The objective was to evaluate the nutritional and physicochemical composition of teosinte flour for the first time. Using diverse techniques, teosinte flour was found to be a high-calorie food rich in total carbohydrates and mainly composed of starch, with an amylopectin:amylose ratio of 2:1 and a concentration of resistant starch greater than 50%. Its proteins were similar to other cereals in which the essential amino acids glutamic acid, leucine, and especially lysine were the most important. Some 75% of its total dietary fiber was insoluble. The fatty acid profile was characterized by a high unsaturated fatty acid content in which oleic acid (C18:1) and linoleic acid (C18:2) predominated. As for minerals, teosinte flour had higher iron content, lower sodium concentration, and similar zinc, calcium, and phosphorus content to other cereal flours. We highlight that teosinte flour has nutrients and qualities that convert it into flour with excellent nutritional abilities and health benefits; it is also a very good industrial and technological alternative to be mixed mainly with types of flour from other sources.

scholarly journals The effects of unsaturated fatty acid depletion on the proton permeability and energetic functions of yeast mitochondria

1977 ◽  
Vol 166 (3) ◽  
pp. 565-570 ◽  
Author(s):  
J. M. Haslam ◽  
Norman F. Fellows
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Acid Content ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Content ◽  
Energy Charge ◽  
Tween 80 ◽  
Adenylate Energy Charge ◽  
Proton Permeability

1. The fatty acid composition of the ole-1 and ole-1 petite mutants of Saccharomyces cerevisiae was manipulated by growing the organism in the presence of defined supplements of Tween 80 or by allowing cells that had first been grown in the presence of Tween 80 to deplete their unsaturated fatty acids by sequent growth in the absence of Tween 80. 2. The transition temperature of Arrhenius plots of mitochondrial ATPase (adenosine triphosphatase) increases as the unsaturated fatty acid content is lowered. 3. Cells require larger amounts of unsaturated fatty acids to grow on ethanol at lower temperatures. 4. Cells that stop growing owing to unsaturated fatty acid depletion at low temperatures are induced to grow further by raising the temperature and this results in a further depletion of unsaturated acids. This is due to a higher rate, but not a greater efficiency, of mitochondrial ATP synthesis. 5. Arrhenius plots of the passive permeability of mitochondria to protons between 4 and 37°C are linear. The rate and the Arrhenius activation energy of proton entry increase greatly as the unsaturated fatty acid content is lowered. 6. Unsaturated fatty acid depletion has the same effects on the proton permeability of ole-1 petite mitochondria, indicating that the mitochondrially synthesized subunits of the ATPase are not involved in the enhanced rates of proton entry. 7. The adenylate energy charge of depleted ole-1 cells is greatly decreased by growth on ethanol medium. 8. The adenylate energy charge of isolated mitochondria is also lowered by unsaturated fatty acid depletion. 9. The results confirm that unsaturated fatty acid depletion uncouples oxidative phosphorylation in yeast both in vivo and in vitro, and is a consequence of changes in the lipid part of the membrane.

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