Insight into calcification of Synechocystis sp. enhanced by extracellular carbonic anhydrase

RSC Advances ◽  
2016 ◽  
Vol 6 (35) ◽  
pp. 29811-29817 ◽  
Author(s):  
Zhen-Ni Yang ◽  
Xiao-Min Li ◽  
Ahmad Umar ◽  
Wen-Hong Fan ◽  
Yao Wang
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Insight Into ◽  
Synechocystis Sp

The mechanism of cyanobacterial calcification was proved to be related to extracellular carbonic anhydrase, which enhanced CaCO3 precipitation through facilitating proton consumption during transformation of bicarbonate to carbon dioxide.

scholarly journals A new insight into role of phosphoketolase pathway in Synechocystis sp. PCC 6803

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Anushree Bachhar ◽  
Jiri Jablonsky
Keyword(s):  
In Silico Analysis ◽  
Bioinformatic Analysis ◽  
Phosphoglycerate Mutase ◽  
Phosphoketolase Pathway ◽  
Multi Level ◽  
Silico Analysis ◽  
Insight Into ◽  
Synechocystis Sp ◽  
Pcc 6803

AbstractPhosphoketolase (PKET) pathway is predominant in cyanobacteria (around 98%) but current opinion is that it is virtually inactive under autotrophic ambient CO2 condition (AC-auto). This creates an evolutionary paradox due to the existence of PKET pathway in obligatory photoautotrophs. We aim to answer the paradox with the aid of bioinformatic analysis along with metabolic, transcriptomic, fluxomic and mutant data integrated into a multi-level kinetic model. We discussed the problems linked to neglected isozyme, pket2 (sll0529) and inconsistencies towards the explanation of residual flux via PKET pathway in the case of silenced pket1 (slr0453) in Synechocystis sp. PCC 6803. Our in silico analysis showed: (1) 17% flux reduction via RuBisCO for Δpket1 under AC-auto, (2) 11.2–14.3% growth decrease for Δpket2 in turbulent AC-auto, and (3) flux via PKET pathway reaching up to 252% of the flux via phosphoglycerate mutase under AC-auto. All results imply that PKET pathway plays a crucial role under AC-auto by mitigating the decarboxylation occurring in OPP pathway and conversion of pyruvate to acetyl CoA linked to EMP glycolysis under the carbon scarce environment. Finally, our model predicted that PKETs have low affinity to S7P as a substrate.

Methanol Production via Bioelectrocatalytic Reduction of Carbon Dioxide: Role of Carbonic Anhydrase in Improving Electrode Performance

2011 ◽  
Vol 14 (4) ◽  
pp. E9 ◽  
Author(s):  
Paul K. Addo ◽  
Robert L. Arechederra ◽  
Abdul Waheed ◽  
James D. Shoemaker ◽  
William S. Sly ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Methanol Production ◽  
Electrode Performance
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