Silver nanoparticle-loaded hydroxyapatite coating: structure, antibacterial properties, and capacity for osteogenic induction in vitro

RSC Advances ◽  
2016 ◽  
Vol 6 (11) ◽  
pp. 8549-8562 ◽  
Author(s):  
Bo Tian ◽  
Wei Chen ◽  
Yufeng Dong ◽  
John V. Marymont ◽  
Yong Lei ◽  
...  
Keyword(s):  
Dental Implants ◽  
Silver Nanoparticle ◽  
Antibacterial Properties ◽  
Hydroxyapatite Coating ◽  
Infection Prophylaxis ◽  
Coating Structure ◽  
Osteogenic Induction

AgNP-HAC has the potential to be used on the surfaces of orthopedic and dental implants for infection prophylaxis.

scholarly journals Antibacterial Properties of Triethoxysilylpropyl Succinic Anhydride Silane (TESPSA) on Titanium Dental Implants

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 773
Author(s):  
Judit Buxadera-Palomero ◽  
Maria Godoy-Gallardo ◽  
Meritxell Molmeneu ◽  
Miquel Punset ◽  
Francisco Javier Gil
Keyword(s):  
Dental Implants ◽  
Photoelectron Spectroscopy ◽  
Succinic Anhydride ◽  
Human Fibroblasts ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Sodium Titanate ◽  
Adhesion Assay ◽  
Vitro Effect

Infections related to dental implants are a common complication that can ultimately lead to implant failure, and thereby carries significant health and economic costs. In order to ward off these infections, this paper explores the immobilization of triethoxysilylpropyl succinic anhydride (TESPSA, TSP) silane onto dental implants, and the interaction of two distinct monospecies biofilms and an oral plaque with the coated titanium samples. To this end, titanium disks from prior machining were first activated by a NaOH treatment and further functionalized with TESPSA silane. A porous sodium titanate surface was observed by scanning electron microscopy and X-ray photoelectron spectroscopy analyses confirmed the presence of TESPSA on the titanium samples (8.4% for Ti–N-TSP). Furthermore, a lactate dehydrogenase assay concluded that TESPSA did not have a negative effect on the viability of human fibroblasts. Importantly, the in vitro effect of modified surfaces against Streptococcus sanguinis, Lactobacillus salivarius and oral plaque were studied using a viable bacterial adhesion assay. A significant reduction was achieved in all cases but, as expected, with different effectiveness against simple mono-species biofilm (ratio dead/live of 0.4) and complete oral biofilm (ratio dead/live of 0.6). Nevertheless, this approach holds a great potential to provide dental implants with antimicrobial properties.

scholarly journals Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sakkarin Bhubhanil ◽  
Chanon Talodthaisong ◽  
Mattaka Khongkow ◽  
Katawut Namdee ◽  
Prapimpun Wongchitrat ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticle ◽  
Guar Gum ◽  
Antibacterial Properties ◽  
Dermal Fibroblasts ◽  
In Vivo Studies ◽  
Great Promise ◽  
Hydrogel Composite

AbstractBiocompatible materials that act as scaffolds for regenerative medicine are of enormous interest. Hydrogel-nanoparticle composites have great potential in this regard, however evaluations of their wound healing and safety in vivo in animal studies are scarce. Here we demonstrate that a guar gum/curcumin-stabilized silver nanoparticle hydrogel composite is an injectable material with exceptional wound healing and antibacterial properties. We show that the curcumin-bound silver nanoparticles themselves exhibit low cytotoxicity and enhance proliferation, migration, and collagen production in in vitro studies of human dermal fibroblasts. We then show that the hydrogel-nanoparticle composite promotes wound healing in in vivo studies on rats, accelerating wound closure by > 40% and reducing bacterial counts by 60% compared to commercial antibacterial gels. Histopathology indicates that the hydrogel composite enhances transition from the inflammation to proliferation stage of healing, promoting the formation of fibroblasts and new blood vessels, while target gene expression studies confirm that the accelerated tissue remodeling occurs along the normal pathways. As such these hydrogel composites show great promise as wound dressing materials with high antibacterial capacity.

scholarly journals Synthesized Silver Nanoparticle Loaded Gel of Curcuma Caesia for Effective Treatment of Acne

2020 ◽  
Vol 10 (6-s) ◽  
pp. 75-82
Author(s):  
Nivedita Rai ◽  
Tarkeshwar P. Shukla ◽  
Kavita R. Loksh ◽  
Sarita Karole
Keyword(s):  
Silver Nanoparticles ◽  
Effective Treatment ◽  
Silver Nanoparticle ◽  
Average Particle Size ◽  
In Vitro Release ◽  
Antibacterial Properties ◽  
In Vitro Study ◽  
Alkaloid Content ◽  
Average Particle

Objective: The objective of our research is to prepare silver nanoparticles from the rhizome extract of Curcuma caesia and develop topical herbal gel formulation for the effective treatment of acne. Methods: In this present study, silver nanoparticles were synthesized using hydroalcoholic extract of Curcuma caesia rhizome. Silver nanoparticles loaded gels were evaluated for pH, viscosity, spreadability, in vitro release, estimation of total flavonoids and alkaloid content and antibacterial (propioni bacterium acne) studies. Results: The synthesized silver nanoparticles were stable, spherical shape with average particle size of 220.5 nm. The results obtained in the developed formulation showed no lumps, had uniform color dispersion and were free from any fiber and particle. It was also observed to have easy washablity, good spreadability, pH was found to be 6.58±0.02 and 7.02±0.01 similar to pH of the skin. The antibacterial study of the developed formulation showed inhibitory activity against Propioni bacterium acne. Synthesized silver nanoparticle loaded gel displayed drug release of optimized formulation F3 follows the Higuchi kinetic model, and the mechanism is found to be non Fickian/anomalous according to Korsmeyer–Peppas. Silver nanoparticles effectively inhibited the growth of both microorganism indicating good antibacterial properties. Conclusion: Synthesis of silver nanoparticles using Curcuma caesia is a new, green method and not reported yet, as per literature survey done for this project. Successful synthesis and evaluation of silver nanoparticles was proved by the in-vitro study. Keywords: Curcuma caesia, Silver nanoparticles, Propioni bacterium acne, Acne, Flavonoids content, Alkaloid content, Antibacterial.

scholarly journals Synthesis of silver nanoparticle-decorated hydroxyapatite nanocomposite with combined bioactivity and antibacterial properties

2021 ◽  
Vol 32 (9) ◽  
Author(s):  
Soo-Ling Bee ◽  
Yazmin Bustami ◽  
A. Ul-Hamid ◽  
Keemi Lim ◽  
Z. A. Abdul Hamid
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticle ◽  
Antibacterial Agents ◽  
Chemical Reduction ◽  
Antibacterial Properties ◽  
In Vitro Bioactivity ◽  
Bone Implant ◽  
Bioactive Material ◽  
Support Matrix

AbstractCombination of bioactive material such as hydroxyapatite (HAp) with antibacterial agents would have great potential to be used as bone implant materials to avert possible bacterial infection that can lead to implant-associated diseases. The present study aimed to develop an antibacterial silver nanoparticle-decorated hydroxyapatite (HAp/AgNPs) nanocomposite using chemical reduction and thermal calcination approaches. In this work, natural HAp that was extracted from chicken bone wastes is used as support matrix for the deposition of silver nanoparticles (AgNPs) to produce HAp/AgNPs nanocomposite. XRD, FESEM-EDX, HRTEM, and XPS analyses confirmed that spherical AgNPs were successfully synthesized and deposited on the surface of HAp particles, and the amount of AgNPs adhered on the HAp surface increased with increasing AgNO3 concentration used. The synthesized HAp/AgNPs nanocomposites demonstrated strong antibacterial activity against Staphylococcus aureus bacteria, where the antibacterial efficiency is relied on the amount and size of deposited AgNPs. In addition, the in vitro bioactivity examination in Hank’s balanced salt solution showed that more apatite were grown on the surface of HAp/AgNPs nanocomposite when AgNO3 concentration used >1 wt.%. Such nanocomposite with enhanced bioactivity and antibacterial properties emerged as a promising biomaterial to be applied for dentistry and orthopedic implantology.

scholarly journals Macrophagic Inflammatory Response Next to Dental Implants with Different Macro- and Micro-Structure: An In Vitro Study

2021 ◽  
Vol 11 (12) ◽  
pp. 5324
Author(s):  
Maria Menini ◽  
Francesca Delucchi ◽  
Domenico Baldi ◽  
Francesco Pera ◽  
Francesco Bagnasco ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Dental Implants ◽  
In Vitro Study ◽  
Titanium Implants ◽  
Implant Surface ◽  
Bone Implant ◽  
Optimal Outcomes ◽  
Negative Controls ◽  
Inflammatory Effect

(1) Background: Intrinsic characteristics of the implant surface and the possible presence of endotoxins may affect the bone–implant interface and cause an inflammatory response. This study aims to evaluate the possible inflammatory response induced in vitro in macrophages in contact with five different commercially available dental implants. (2) Methods: one zirconia implant NobelPearl® (Nobel Biocare) and four titanium implants, Syra® (Sweden & Martina), Prama® (Sweden & Martina), 3iT3® (Biomet 3i) and Shard® (Mech & Human), were evaluated. After 4 h of contact of murine macrophage cells J774a.1 with the implants, the total RNA was extracted, transcribed to cDNA and the gene expression of the macrophages was evaluated by quantitative PCR (qPCR) in relation to the following genes: GAPDH, YWHAZ, IL1β, IL6, TNFα, NOS2, MMP-9, MMP-8 and TIMP3. The results were statistically analyzed and compared with negative controls. (3) Results: No implant triggered a significant inflammatory response in macrophages, although 3iT3 exhibited a slight pro-inflammatory effect compared to other samples. (4) Conclusions: All the samples showed optimal outcomes without any inflammatory stimulus on the examined macrophagic cells.

scholarly journals Comparison of irrigation protocols for the internal decontamination of dental implants – results of in‐vitro and in‐vivo studies

2021 ◽  
Author(s):  
Pia‐Merete Jervøe‐Storm ◽  
Alexandra Selina Hablützel ◽  
Philipp Bartels ◽  
Dominik Kraus ◽  
Søren Jepsen ◽  
...  
Keyword(s):  
Dental Implants ◽  
In Vivo Studies

scholarly journals The Biological Activity of Monarda didyma L. Essential Oil and Its Effect as a Diet Supplement in Mice and Broiler Chicken

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3368
Author(s):  
Héloïse Côté ◽  
André Pichette ◽  
Alexis St-Gelais ◽  
Jean Legault
Keyword(s):  
Body Weight ◽  
Essential Oil ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Growth Promoters ◽  
Antibiotic Growth Promoters ◽  
Monarda Didyma

The use of growth-promoting antibiotics in livestock faces increasing scrutiny and opposition due to concerns about the increased occurrence of antibiotic-resistant bacteria. Alternative solutions are being sought, and plants of Lamiaceae may provide an alternative to synthetic antibiotics in animal nutrition. In this study, we extracted essential oil from Monarda didyma, a member of the Lamiaceae family. We examined the chemical composition of the essential oil and then evaluated the antibacterial, antioxidant, and anti-inflammatory activities of M. didyma essential oil and its main compounds in vitro. We then evaluated the effectiveness of M. didyma essential oil in regard to growth performance, feed efficiency, and mortality in both mice and broilers. Carvacrol (49.03%) was the dominant compound in the essential oil extracts. M. didyma essential oil demonstrated antibacterial properties against Escherichia coli (MIC = 87 µg·mL−1), Staphylococcus aureus (MIC = 47 µg·mL−1), and Clostridium perfringens (MIC = 35 µg·mL−1). Supplementing the diet of mice with essential oil at a concentration of 0.1% significantly increased body weight (+5.4%) and feed efficiency (+18.85%). In broilers, M. didyma essential oil significantly improved body weight gain (2.64%). Our results suggest that adding M. didyma essential oil to the diet of broilers offers a potential substitute for antibiotic growth promoters.

scholarly journals A High-Throughput Metabolic Microarray Assay Reveals Antibacterial Effects of Black and Red Raspberries and Blackberries against Helicobacter pylori Infection

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 845
Author(s):  
Candace Goodman ◽  
Katrina N. Lyon ◽  
Aitana Scotto ◽  
Cyra Smith ◽  
Thomas A. Sebrell ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
High Throughput ◽  
Antibacterial Properties ◽  
Treatment Strategies ◽  
Helicobacter Pylori Infection ◽  
Antibacterial Effects ◽  
Biolog System ◽  
Freeze Dried ◽  
H Pylori

Helicobacter pylori infection is commonly treated with a combination of antibiotics and proton pump inhibitors. However, since H. pylori is becoming increasingly resistant to standard antibiotic regimens, novel treatment strategies are needed. Previous studies have demonstrated that black and red berries may have antibacterial properties. Therefore, we analyzed the antibacterial effects of black and red raspberries and blackberries on H. pylori. Freeze-dried powders and organic extracts from black and red raspberries and blackberries were prepared, and high-performance liquid chromatography was used to measure the concentrations of anthocyanins, which are considered the major active ingredients. To monitor antibiotic effects of the berry preparations on H. pylori, a high-throughput metabolic growth assay based on the Biolog system was developed and validated with the antibiotic metronidazole. Biocompatibility was analyzed using human gastric organoids. All berry preparations tested had significant bactericidal effects in vitro, with MIC90 values ranging from 0.49 to 4.17%. Antimicrobial activity was higher for extracts than powders and appeared to be independent of the anthocyanin concentration. Importantly, human gastric epithelial cell viability was not negatively impacted by black raspberry extract applied at the concentration required for complete bacterial growth inhibition. Our data suggest that black and red raspberry and blackberry extracts may have potential applications in the treatment and prevention of H. pylori infection but differ widely in their MICs. Moreover, we demonstrate that the Biolog metabolic assay is suitable for high-throughput antimicrobial susceptibility screening of H. pylori.

Sign in / Sign up

Export Citation Format

Share Document