Enhanced in vivo tumour imaging by EDTA-bis-GNGR functionalized core shell CdSe:ZnS quantum dot: synergistic effect of active passive targeting

RSC Advances ◽  
2016 ◽  
Vol 6 (16) ◽  
pp. 13562-13571 ◽  
Author(s):  
Rashi Mathur ◽  
Narmada Bag ◽  
Raunak Varshney ◽  
Firasat Hussain ◽  
Ankur Kaul ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Synergistic Effect ◽  
Core Shell ◽  
Tumour Targeting ◽  
Tumour Imaging ◽  
Passive Targeting

Use of QD-homodimeric system enhances tumour targeting due to the synergistic effect of active passive targeting.

Multifunctional gold nanoparticles as smart nanovehicles with enhanced tumour-targeting abilities for intracellular pH mapping and in vivo MR/fluorescence imaging

Nanoscale ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 2002-2010 ◽  
Author(s):  
Kang-Kang Yu ◽  
Kun Li ◽  
Chun-Yan Lu ◽  
Yong-Mei Xie ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Imaging ◽  
Intracellular Ph ◽  
Living Cells ◽  
Tumour Targeting ◽  
Tumour Imaging ◽  
Pathological Changes

A number of multimodal agents have been developed for tumour imaging and diagnosis, but most of them cannot be used to study the detailed physiological or pathological changes in living cells at the same time.

Core-shell biodegradable nanoassemblies for the passive targeting of docetaxel: features, antiproliferative activity and in vivo toxicity

2012 ◽  
Vol 8 (5) ◽  
pp. 637-646 ◽  
Author(s):  
Francesca Ungaro ◽  
Claudia Conte ◽  
Luisanna Ostacolo ◽  
Giovanni Maglio ◽  
Antonio Barbieri ◽  
...  
Keyword(s):  
Antiproliferative Activity ◽  
Core Shell ◽  
Passive Targeting ◽  
In Vivo Toxicity

scholarly journals Synthesis and in vivo toxicity assessment of CdSe:ZnS quantum dots functionalized with EDTA-Bis-Cysteamine

2015 ◽  
Vol 4 (5) ◽  
pp. 1416-1425 ◽  
Author(s):  
Narmada Bag ◽  
Rashi Mathur ◽  
Firasat Hussain ◽  
Namita Indracanti ◽  
Sweta Singh ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Quantum Dot ◽  
Toxicity Assessment ◽  
Core Shell ◽  
In Vivo Toxicity

Synthesis and in vivo toxicity assessment of radiolabeled Bis-ligand functionalized core shell quantum dot.

scholarly journals Evaluation of CdTe/CdS/ZnS core/shell/shell quantum dot toxicity on three-dimensional spheroid cultures

2016 ◽  
Vol 5 (1) ◽  
pp. 126-135 ◽  
Author(s):  
Mehriban Ulusoy ◽  
Antonina Lavrentieva ◽  
Johanna-Gabriela Walter ◽  
Franziska Sambale ◽  
Mark Green ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Quantum Dot ◽  
Three Dimensional ◽  
Core Shell ◽  
Tissue Structures ◽  
Cytotoxicity Effects ◽  
Dose Dependent

Three-dimensional hAD-MSCs spheroid cultures, with the ability to resemble closely in vivo tissue structures, were assessed for evaluation of dose-dependent cytotoxicity effects of CdTe/CdS/ZnS quantum dots.

Quantum Dot Nanocrystals for In Vivo Molecular and Cellular Imaging¶

2004 ◽  
Vol 80 (3) ◽  
pp. 377 ◽  
Author(s):  
Andrew M. Smith ◽  
Xiaohu Gao ◽  
Shuming Nie
Keyword(s):  
Quantum Dot ◽  
Cellular Imaging

Human Fibronectin Extra-Domain B (EDB)-Specific Aptide (APTEDB) Radiolabelling with Technetium-99m as a Potent Targeted Tumour-Imaging Agent

2018 ◽  
Vol 18 (2) ◽  
pp. 277-285 ◽  
Author(s):  
Mohsen Mohammadgholi ◽  
Nourollah Sadeghzadeh ◽  
Mostafa Erfani ◽  
Saeid Abediankenari ◽  
Seyed Mohammad Abedi ◽  
...  
Keyword(s):  
Radioligand Binding ◽  
Specific Binding ◽  
Specific Protein ◽  
Tumour Imaging ◽  
Specific Binding Ratio ◽  
Tumour Uptake ◽  
Technetium 99M ◽  
In Vivo Experiments ◽  
Human Fibronectin

Background: Human fibronectin extra-domain B (EDB) is particularly expressed during angiogenesis progression. It is, thus, a promising marker of tumour growth. Aptides are a novel class of peptides with high-affinity binding to specific protein targets. APTEDB is an antagonist-like ligand that especially interacts with human fibronectin EDB. Objective: This study was the first attempt in which the hydrazinonicotinamide (HYNIC)-conjugated APTEDB was labelled with technetium-99m (99mTc) as an appropriate radiotracer and tricine/EDDA exchange labeling. Methods: Radiochemical purity, normal saline, and serum stability were evaluated by HPLC and radio-isotope TLC scanner. Other examinations, such as protein-binding calculation, dissociation radioligand binding assay, and partition coefficient constant determination, were also carried out. The cellular-specific binding of 99mTc- HYNIC-conjugated APTEDB was assessed in two EDB-positive (U87MG) and EDB-negative (U373MG) cell lines. Bio-distribution was investigated in normal mice as well as in U87MG and U373MG tumour-bearing mice. Eventually, the radiolabelled APTEDB was used for tumour imaging using planar SPECT. Results: Radiolabelling was achieved with high purity (up to 97%) and accompanied by high solution (over 90% after overnight) and serum (80% after 2 hours) stability. The obtained cellular-specific binding ratio was greater than nine-fold. In-vivo experiments showed rapid blood clearance with mainly renal excretion and tumour uptake specificity (0.48±0.03% ID/g after 1h). The results of the imaging also confirmed considerable tumour uptake for EDB-positive cell line compared with the EDB-negative one. Conclusion: Aptides are considered to be a potent candidate for biopharmaceutical applications. They can be modified with imaging or therapeutic agents. This report shows the capability of 99mTc-HYNIC-APTEDB for human EDB-expressing tumours detection.

scholarly journals The SOC1-like gene BoMADS50 is associated with the flowering of Bambusa oldhamii

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Dan Hou ◽  
Ling Li ◽  
Tengfei Ma ◽  
Jialong Pei ◽  
Zhongyu Zhao ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Large Scale ◽  
Flowering Plants ◽  
Activation Functions ◽  
Regulatory Activity ◽  
Mature Leaves ◽  
Sporadic Flowering ◽  
Bamboo Flowering ◽  
Bambusa Oldhamii

AbstractBamboo is known for its edible shoots and beautiful texture and has considerable economic and ornamental value. Unique among traditional flowering plants, many bamboo plants undergo extensive synchronized flowering followed by large-scale death, seriously affecting the productivity and application of bamboo forests. To date, the molecular mechanism of bamboo flowering characteristics has remained unknown. In this study, a SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)-like gene, BoMADS50, was identified from Bambusa oldhamii. BoMADS50 was highly expressed in mature leaves and the floral primordium formation period during B. oldhamii flowering and overexpression of BoMADS50 caused early flowering in transgenic rice. Moreover, BoMADS50 could interact with APETALA1/FRUITFULL (AP1/FUL)-like proteins (BoMADS14-1/2, BoMADS15-1/2) in vivo, and the expression of BoMADS50 was significantly promoted by BoMADS14-1, further indicating a synergistic effect between BoMADS50 and BoAP1/FUL-like proteins in regulating B. oldhamii flowering. We also identified four additional transcripts of BoMADS50 (BoMADS50-1/2/3/4) with different nucleotide variations. Although the protein-CDS were polymorphic, they had flowering activation functions similar to those of BoMADS50. Yeast one-hybrid and transient expression assays subsequently showed that both BoMADS50 and BoMADS50-1 bind to the promoter fragment of itself and the SHORT VEGETATIVE PHASE (SVP)-like gene BoSVP, but only BoMADS50-1 can positively induce their transcription. Therefore, nucleotide variations likely endow BoMADS50-1 with strong regulatory activity. Thus, BoMADS50 and BoMADS50-1/2/3/4 are probably important positive flowering regulators in B. oldhamii. Moreover, the functional conservatism and specificity of BoMADS50 and BoMADS50-1 might be related to the synchronized and sporadic flowering characteristics of B. oldhamii.

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