Qualitative and quantitative determinations of pyridalyl and metabolites in excrement of two representative Lepidoptera pests

RSC Advances ◽  
2015 ◽  
Vol 5 (125) ◽  
pp. 103474-103479 ◽  
Author(s):  
Lingling Wang ◽  
Yuting Nie ◽  
Yujiao Wang ◽  
Zhenyu Wang ◽  
Bo Xiong
Keyword(s):  
Helicoverpa Armigera ◽  
Spodoptera Exigua ◽  
Qualitative And Quantitative ◽  
Helicoverpa Armígera ◽  
Tof Ms

Qualitative and quantitative SPE followed by HPLC-TOF/MS determination of pyridalyl and its potential metabolites in the excrement of Helicoverpa armigera (H. armigera) and Spodoptera exigua (S. exigua) was developed.

scholarly journals Common Receptor for Bacillus thuringiensis Toxins Cry1Ac, Cry1Fa, and Cry1Ja in Helicoverpa armigera, Helicoverpa zea, and Spodoptera exigua

2005 ◽  
Vol 71 (9) ◽  
pp. 5627-5629 ◽  
Author(s):  
Carmen Sara Hernández ◽  
Juan Ferré
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
Spodoptera Exigua ◽  
Cross Resistance ◽  
Binding Studies ◽  
Lepidopteran Species ◽  
Common Receptor ◽  
Binding Data ◽  
Helicoverpa Armígera

ABSTRACT Binding studies using 125I-Cry1Ac and biotinylated Cry1Fa toxins indicate the occurrence of a common receptor for Cry1Ac, Cry1Fa, and Cry1Ja in Helicoverpa armigera, Helicoverpa zea, and Spodoptera exigua. Our results, along with previous binding data and the observed cases of cross-resistance, suggest that this pattern seems to be widespread among lepidopteran species.

scholarly journals The F protein of Helicoverpa armigera single nucleopolyhedrovirus can be substituted functionally with its homologue from Spodoptera exigua multiple nucleopolyhedrovirus

2008 ◽  
Vol 89 (3) ◽  
pp. 791-798 ◽  
Author(s):  
Manli Wang ◽  
Ying Tan ◽  
Feifei Yin ◽  
Fei Deng ◽  
Just M. Vlak ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Spodoptera Exigua ◽  
Late Phase ◽  
Positive Control ◽  
Host Proteins ◽  
F Protein ◽  
Helicoverpa Armígera ◽  
Group Ii ◽  
Budded Virus ◽  
Species Specific

F proteins of group II nucleopolyhedroviruses (NPVs) are envelope fusion proteins essential for virus entry and egress. An F-null Helicoverpa armigera single nucleocapsid NPV (HearNPV) bacmid, HaBacΔF, was constructed. This bacmid could not produce infectious budded virus (BV) when transfected into HzAM1 cells, showing that F protein is essential for cell-to-cell transmission of BVs. When HaBacΔF was pseudotyped with the homologous F protein (HaBacΔF-HaF, positive control) or with the heterologous F protein from Spodoptera exigua multinucleocapsid NPV (SeMNPV) (HaBacΔF-SeF), infectious BVs were produced with similar kinetics. In the late phase of infection, the BV titre of HaBacΔF-SeF virus was about ten times lower than that of HaBacΔF-HaF virus. Both pseudotyped viruses were able to fuse HzAM1 cells in a similar fashion. The F proteins of both HearNPV and SeMNPV were completely cleaved into F1 and F2 in the BVs of vHaBacΔF-HaF and vHaBacΔF-SeF, respectively, but the cleavage of SeF in vHaBacΔF-SeF-infected HzAM1 cells was incomplete, explaining the lower BV titre of vHaBacΔF-SeF. Polyclonal antisera against HaF1 and SeF1 specifically neutralized the infection of vHaBacΔF-HaF and vHaBacΔF-SeF, respectively. HaF1 antiserum showed some cross-neutralization with vHaBacΔF-SeF. These results demonstrate that group II NPV F proteins can be functionally replaced with a homologue of other group II NPVs, suggesting that the interaction of F with other viral or host proteins is not absolutely species-specific.

scholarly journals EFEKTIVITAS BEBERAPA PREDATOR TERHADAP Helicoverpa armigera (HÜBNER) PADA KAPAS TUMPANGSARI DENGAN KEDELAI

2020 ◽  
Vol 12 (3) ◽  
pp. 109
Author(s):  
NURINDAH NURINDAH ◽  
DWI ADI SUNARTO
Keyword(s):  
Gossypium Hirsutum ◽  
Seed Production ◽  
Helicoverpa Armigera ◽  
Cotton Seed ◽  
Mortality Factor ◽  
Gossypium Hirsutum L ◽  
Threshold Concept ◽  
Action Threshold ◽  
Helicoverpa Armígera

ABSTRAK<br />Penelitian ini bertujuan untuk mengevaluasi efektivitas beberapa<br />predator terhadap H. armigera pada kapas. Penelitian dilakukan pada<br />pertanaman kapas di daerah pengembangan Lamongan, Jawa Tmur.<br />Kegiatan penelitian terdiri atas dua kegiatan, yaitu penentuan faktor<br />mortalitas biotik H. armigera dan perkembangan populasi H. armigera<br />pada lahan tanpa penyemprotan insektisida. Kegiatan penentuan faktor<br />mortalitas biotik H. armigera dilakukan pada 5 lahan tanpa penyemprotan<br />insektisida masing-masing seluas 100 m 2 dengan mengamati populasi H.<br />armigera dan predatornya. Kegiatan perkembangan populasi H. armigera<br />pada lahan tanpa penyemprotan insektisida dilakukan pada lahan seluas 5<br />hektar dengan mengamati populasi serangga hama, predator, dan hasil<br />kapas berbiji pada setiap sub petak yang luasnya 0,2 ha. Hasil penelitian<br />menunjukkan bahwa kompleks predator yang terdiri dari kumbang kubah,<br />semut, kepik mirid dan laba-laba berperan dalam pengaturan populasi H.<br />armigera. Kumbang kubah dan semut merupakan faktor mortalitas yang<br />penting bagi telur H. armigera, sedangkan kompleks predator merupakan<br />faktor mortalitas yang penting bagi larva kecil H. armigera. Persamaan<br />regresi hubungan antara kompleks predator dan larva H. armigera adalah<br />y= 3796 – 0.431x, sehingga dapat diartikan bahwa satu larva H. armigera<br />dapat dieliminasi oleh 8 ekor predator. Jika keberadaan predator<br />dipertimbangkan dalam ambang kendali H. armigera, maka pada sistem<br />tanam kapas tumpangsari dengan kedelai, populasi H. armigera tidak<br />pernah mencapai ambang kendali. Produksi kapas berbiji pada lahan tanpa<br />penyemprotan insektisida adalah 911 kg/ha; hasil ini jauh lebih tinggi<br />dibandingkan dengan produksi dari lahan dengan pengendalian secara<br />konvensional (penyemprotan insektisida secara intensif) yang hanya<br />mencapai 312 kg/ha. Oleh karena itu, keberadaan predator perlu<br />dipertimbangkan dalam konsep ambang kendali H. armigera.<br />Kata kunci : Kapas, Gossypium hirsutum L., Helicoverpa armigera,<br />predator, ambang kendali, produksi, Jawa Timur<br />ABSTRACT<br />Effectiveness of predators on Helicoverpa armigera on<br />cotton intercropped with soybean<br />Research on effectiveness of predators of H. amigera on cotton<br />intercropped with soybean was conducted in Lamongan, East Java. The<br />research activity included determination of the biotic mortality factor of H.<br />armigera and observation on H. armigera population in unsprayed cotton<br />field. Determination of the biotic mortality factor of H. armigera was<br />conducted in 5 unsprayed plots @ 100 m 2 by recording the number of H.<br />armigera and its predators. Observation on H. armigera population was<br />done in 5 hectares of unsprayed cotton field by observing the population of<br />H. armigera and its predators as well as cotton seed production of each 0.2<br />hectare sub plot. The research results showed that predator complex<br />(Coccinellid beetles, mirid bugs, ants and spiders) have significant role in<br />regulating H. armigera population. Coccinellid beetles and ants were the<br />important biotic mortality factors of H. armigera eggs, while complex<br />predators were that of the small larvae. Regression equation of predator<br />complex and H. armigera larvae was y= 3.976 – 0.43x, meaning that one<br />H. armigera larvae could be eliminated by 8 predators. If the presence of<br />predators was considered in action threshold concept, population of H.<br />armigera on cotton intercropped with soybean was always under action<br />threshold. Cotton seed production of unsprayed field was 911 kg/ha; this<br />was significantly higher than that of conventional control system with<br />intensive sprays of chemical insecticides. Therefore, the presence of<br />predators should be included in action threshold concept of H. armigera.<br />Key words : Cotton, Gossypium hirsutum L., pest, Helicoverpa armigera,<br />predator, action threshold, production, East Java

scholarly journals Qualitative and Quantitative Analysis ofEclipta prostrataL. by LC/MS

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Lifeng Han ◽  
Erwei Liu ◽  
Agyemang Kojo ◽  
Jing Zhao ◽  
Wei Li ◽  
...  
Keyword(s):  
Uterine Bleeding ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Echinocystic Acid ◽  
Eclipta Prostrata ◽  
Quantitative Analyses ◽  
Accuracy And Repeatability ◽  
Good Linear Correlation ◽  
Tof Ms

Eclipta prostrataL. is one of the Chinese medicinal tonics which are usually used for treating loose teeth, dizziness, tinnitus, hemoptysis, hematuria, and uterine bleeding. However, quality control of this herbal medicine has been not satisfactory. This study reported its qualitative and quantitative analyses based on LC/MS method. UHPLC-DAD-Q-TOF-MS fingerprinting and MS fragmentation cleavage pathway were investigated for qualitative analysis. Furthermore, a method for simultaneous quantitative determination of nine compounds, luteolin 7-O-β-D-glucopyranoside, ecliptasaponin C, luteolin, eclalbasaponin IV, apigenin, ecliptasaponin A, echinocystic acid 28-O-β-D-glucopyranoside, echinocystic acid, and 3-oxo-16α-hydroxy-olean-12-en-28-oic acid inE. prostrata, was established. The method was validated for samples ofE. prostratafrom different habitats. The results showed good linear correlation, precision, accuracy, and repeatability that could be used for contents determination of the nine compounds inE. prostratafrom different habitats.

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