Biotreatment of simulated tannery wastewater containing Reactive Black 5, aniline and CrVI using a biochar packed bioreactor

RSC Advances ◽  
2015 ◽  
Vol 5 (128) ◽  
pp. 106272-106279 ◽  
Author(s):  
Shahid Mahmood ◽  
Azeem Khalid ◽  
Tariq Mahmood ◽  
Muhammad Arshad ◽  
Juan Carlos Loyola-Licea ◽  
...  
Keyword(s):  
Azo Dyes ◽  
Dye Decolorization ◽  
Packed Bed ◽  
Tannery Wastewater ◽  
Reactive Black 5 ◽  
Anaerobic Conditions ◽  
Redox Active ◽  
Support Matrix ◽  
Active Bacteria

Azo dyes and CrVI in tannery wastewater can be treated by redox active bacteria. Dye decolorization and CrVI reduction are simultaneous under anaerobic conditions. Biochar is an effective support matrix for packed bed bioreactors used to treat dyes and CrVI.

Differential Protein Expression in Shewanella seohaensis Decolorizing Azo Dyes

2019 ◽  
Vol 16 (2) ◽  
pp. 156-164 ◽  
Author(s):  
Nadine Ana de Souza ◽  
Nagappa Ramaiah ◽  
Samir Damare ◽  
Bliss Furtado ◽  
Chellandi Mohandass ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Azo Dyes ◽  
Azo Dye ◽  
Dye Decolorization ◽  
Protein Quantification ◽  
Reactive Black 5 ◽  
Safe Alternative ◽  
Wide Scale ◽  
Reactive Green 19 ◽  
Azo Dye Decolorization

Background:Microbial remediation is an ecologically safe alternative to controlling environmental pollution caused by toxic aromatic compounds including azo dyes. Marine bacteria show excellent potential as agents of bioremediation. However, a lack of understanding of the entailing mechanisms of microbial degradation often restricts its wide-scale and effective application.Objective:To understand the changes in a bacterial proteome profile during azo dye decolorization.Methods:In this study, we tested a Gram-negative bacterium, Shewanella seohaensis NIODMS14 isolated from an estuarine environment and grown in three different azo dyes (Reactive Black 5 (RB5), Reactive Green 19 (RG19) and Reactive Red 120 (RR120)). The unlabeled bacterial protein samples extracted during the process of dye decolorization were subject to mass spectrometry. Relative protein quantification was determined by comparing the resultant MS/MS spectra for each protein.Results:Maximum dye decolorization of 98.31% for RB5, 91.49% for RG19 and 97.07% for RR120 at a concentration of 100 mg L-1 was observed. The liquid chromatography-mass spectrometry - Quadrupole Time of Flight (LCMS-QToF) analysis revealed that as many as 29 proteins were up-regulated by 7 hours of growth and 17 by 24 hours of growth. Notably, these were common across the decolorized solutions of all three azo dyes. In cultures challenged with the azo dyes, the major class of upregulated proteins was cellular oxidoreductases and an alkyl hydroperoxide reductase (SwissProt ID: A9KY42).Conclusion:The findings of this study on the bacterial proteome profiling during the azo dye decolorization process are used to highlight the up-regulation of important proteins that are involved in energy metabolism and oxido-reduction pathways. This has important implications in understanding the mechanism of azo dye decolorization by Shewanella seohaensis.

scholarly journals Comparative Anaerobic Decolorization of Azo Dyes by Carbon-Based Membrane Bioreactor

Water ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1060
Author(s):  
Mohammad Shaiful Alam Amin ◽  
Frank Stüber ◽  
Jaume Giralt ◽  
Agustin Fortuny ◽  
Azael Fabregat ◽  
...  
Keyword(s):  
Azo Dyes ◽  
Cost Effective ◽  
Dye Decolorization ◽  
Feed Solution ◽  
Reactive Black 5 ◽  
Permeate Flux ◽  
Acid Orange 7 ◽  
Carbon Membrane ◽  
Feed Concentration ◽  
Biofilm Support

This study used a novel integrated technology of ceramic supported carbon membrane (CSCM) to degrade azo dyes through an anaerobic mixed culture. The CSCM worked simultaneously as biofilm support, redox mediator, and nano-filter to enhance the dye decolorization efficiency. The decolorization of Acid Orange 7 (AO7) was initially investigated with and without microorganisms in both ceramic support (CS) and CSCM reactors. The CSCM bioreactor (B-CSCM), operated with microorganisms, gave a maximum decolorization of 98% using a CSCM evolved from 10% weight (wt.) of Matrimid 5218 solution. To know the influence of permeate flow, feed concentration, and dye structure on the decolorization process, different B-CSCMs for dye removal experiments were studied over monoazo AO7, diazo Reactive Black 5 (RB5), and triazo Direct Blue 71 (DB71). The highest color removal, operated with 50 mg·L−1 feed solution and 0.05 L·m−2·h−1 of permeate flux, was 98%, 82%, and 72%, respectively, for AO7, RB5, and DB71. By increasing these parameters to 100 mg·L−1 and 0.1 L·m−2·h−1, the decolorization rate of dye solution still achieved 37% for AO7, 30% for RB5, and 26% for DB71. In addition, the system was run for weeks without apparent loss of activity. These findings make evident that the combined phenomena taking place in CSCM bioreactor result in an efficient, cost-effective, and ecofriendly azo dye decolorization method.

scholarly journals Identification of Quinoide Redox Mediators That Are Formed during the Degradation of Naphthalene-2-Sulfonate by Sphingomonas xenophaga BN6

2002 ◽  
Vol 68 (9) ◽  
pp. 4341-4349 ◽  
Author(s):  
Andreas Keck ◽  
Jörg Rau ◽  
Thorsten Reemtsma ◽  
Ralf Mattes ◽  
Andreas Stolz ◽  
...  
Keyword(s):  
Azo Dyes ◽  
High Performance ◽  
Wild Type Strain ◽  
Gene Replacement ◽  
Redox Mediators ◽  
Liquid Chromatography Mass Spectrometry ◽  
Anaerobic Conditions ◽  
Dioxygenase Gene ◽  
Sphingomonas Xenophaga ◽  
Culture Supernatants

ABSTRACT During aerobic degradation of naphthalene-2-sulfonate (2NS), Sphingomonas xenophaga strain BN6 produces redox mediators which significantly increase the ability of the strain to reduce azo dyes under anaerobic conditions. It was previously suggested that 1,2-dihydroxynaphthalene (1,2-DHN), which is an intermediate in the degradative pathway of 2NS, is the precursor of these redox mediators. In order to analyze the importance of the formation of 1,2-DHN, the dihydroxynaphthalene dioxygenase gene (nsaC) was disrupted by gene replacement. The resulting strain, strain AKE1, did not degrade 2NS to salicylate. After aerobic preincubation with 2NS, strain AKE1 exhibited much higher reduction capacities for azo dyes under anaerobic conditions than the wild-type strain exhibited. Several compounds were present in the culture supernatants which enhanced the ability of S. xenophaga BN6 to reduce azo dyes under anaerobic conditions. Two major redox mediators were purified from the culture supernatants, and they were identified by high-performance liquid chromatography-mass spectrometry and comparison with chemically synthesized standards as 4-amino-1,2-naphthoquinone and 4-ethanolamino-1,2-naphthoquinone.

scholarly journals Characterization and Dye Decolorization Potential of Two Laccases from the Marine-Derived Fungus Pestalotiopsis sp.

2019 ◽  
Vol 20 (8) ◽  
pp. 1864 ◽  
Author(s):  
Wikee ◽  
Hatton ◽  
Turbé-Doan ◽  
Mathieu ◽  
Daou ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Catalytic Efficiency ◽  
Dye Decolorization ◽  
Sea Salt ◽  
Reactive Black 5 ◽  
Surface Charges ◽  
Recombinant Enzymes ◽  
Charged Surfaces ◽  
Three Dimensional Models ◽  
Encoding Genes

: Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, their ability to decolorize textile dyes for potential biotechnological applications, and their activity in the presence of sea salt. The optimal pH and temperature of PsLac1 and PsLac2 differed in relation to the substrates tested, and both enzymes were shown to be extremely stable at temperatures up to 50 °C, retaining 100% activity after 3 h at 50 °C. Both enzymes were stable between pH 4–6. Different substrate specificities were exhibited, and the lowest Km and highest catalytic efficiency values were obtained against syringaldazine and 2,6-dimethoxyphenol (DMP) for PsLac1 and PsLac2, respectively. The industrially important dyes—Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5—were more efficiently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). Activities were compared in saline conditions, and PsLac2 seemed more adapted to the presence of sea salt than PsLac1. The overall surface charges of the predicted PsLac three-dimensional models showed large negatively charged surfaces for PsLac2, as found in proteins for marine organisms, and more balanced solvent exposed charges for PsLac1, as seen in proteins from terrestrial organisms.

scholarly journals Magnetically retrievable Ce-doped Fe3O4 nanoparticles as scaffolds for the removal of azo dyes

RSC Advances ◽  
2019 ◽  
Vol 9 (40) ◽  
pp. 23129-23141 ◽  
Author(s):  
Aashima Aashima ◽  
Shivani Uppal ◽  
Arushi Arora ◽  
Sanjeev Gautam ◽  
Suman Singh ◽  
...  
Keyword(s):  
Azo Dyes ◽  
Fe3o4 Nanoparticles ◽  
Azo Dye ◽  
Reactive Black 5

Considering the significant impact of magnetically retrievable nanostructures, herein, Ce-doped Fe3O4 nanoparticles were employed as scaffolds for the removal of the Reactive Black 5 (RB5), an azo dye.

Characterization of a new oxygen-insensitive azoreductase from Brevibacillus laterosporus TISTR1911: Toward dye decolorization using a packed-bed metal affinity reactor

2013 ◽  
Vol 150 ◽  
pp. 298-306 ◽  
Author(s):  
Weeranuch Lang ◽  
Sarote Sirisansaneeyakul ◽  
Lukana Ngiwsara ◽  
Sónia Mendes ◽  
Lígia O. Martins ◽  
...  
Keyword(s):  
Dye Decolorization ◽  
Packed Bed ◽  
Metal Affinity ◽  
Brevibacillus Laterosporus

Mediator-Based Decolorization of Recalcitrant Dyes with Laccase from Bacillus amyloliquefaciens LS01

2011 ◽  
Vol 183-185 ◽  
pp. 768-772 ◽  
Author(s):  
Lei Lu ◽  
Min Zhao ◽  
De Bin Li ◽  
Li Yan Zhao ◽  
Mei Hui Du ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Dye Decolorization ◽  
Brilliant Blue ◽  
Reactive Black 5 ◽  
Synthetic Dyes ◽  
Remazol Brilliant Blue R ◽  
Mediator System ◽  
Laccase Mediator System

The spore laccase of Bacillus amyloliquefaciens LS01 was evaluated for its ability in decolorization of different synthetic dyes. The decolorization process was not efficient by the laccase alone. The addition of mediators could remarkably improve the efficiency of dye decolorization. Remazol Brilliant Blue R and reactive black 5 were resistant to decolorization for most mediators. Acetosyringone was proved to be the best mediator for the spore laccase, and a decolorization of 63−82% was achieved for all the tested dyes in the presence of acetosyringone. The results indicate that the spore laccase-mediator system could be used for the treatment of industrial dye effluents.

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