Encapsulation of photoactive porphyrinoids in polyelectrolyte hollow microcapsules viewed by fluorescence lifetime imaging microscopy (FLIM)

RSC Advances ◽  
2015 ◽  
Vol 5 (96) ◽  
pp. 79050-79060 ◽  
Author(s):  
Raquel Teixeira ◽  
Vanda Vaz Serra ◽  
Pedro M. R. Paulo ◽  
Suzana M. Andrade ◽  
Sílvia M. B. Costa
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Layer By Layer ◽  
Styrene Sulfonate ◽  
Lifetime Imaging ◽  
Allylamine Hydrochloride ◽  
Poly Styrene Sulfonate

Fluorescence Lifetime Imaging Microscopy (FLIM) was used to investigate the encapsulation of porphyrinoids in multilayer hollow microcapsules assembled layer by layer with poly(styrene sulfonate) (PSS) and poly(allylamine hydrochloride) (PAH).

scholarly journals Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

2016 ◽  
Vol 52 (24) ◽  
pp. 4458-4461 ◽  
Author(s):  
Hideki Itoh ◽  
Satoshi Arai ◽  
Thankiah Sudhaharan ◽  
Sung-Chan Lee ◽  
Young-Tae Chang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Heat Production ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
C2c12 Myotube

FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.

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