Biomolecule incorporated poly-ε-caprolactone nanofibrous scaffolds for enhanced human meniscal cell attachment and proliferation

RSC Advances ◽  
2015 ◽  
Vol 5 (90) ◽  
pp. 73552-73561 ◽  
Author(s):  
J. Gopinathan ◽  
Steffie Mano ◽  
V. Elakkiya ◽  
Mamatha M. Pillai ◽  
K. Santosh Sahanand ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Meniscal Cell ◽  
Nanofibrous Scaffolds

Biomolecule incorporated PCL nanofibrous scaffolds supporting meniscal cell attachment and proliferation.

Silk–PVA Hybrid Nanofibrous Scaffolds for Enhanced Primary Human Meniscal Cell Proliferation

2016 ◽  
Vol 249 (6) ◽  
pp. 813-822 ◽  
Author(s):  
Mamatha M. Pillai ◽  
J. Gopinathan ◽  
B. Indumathi ◽  
Y. R. Manjoosha ◽  
K. Santosh Sahanand ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Meniscal Cell ◽  
Nanofibrous Scaffolds

Mechanics and Cytocompatibility of Genipin Crosslinked Type I Collagen Nanofibrous Scaffolds

2008 ◽  
Author(s):  
Albert O. Gee ◽  
Brendon M. Baker ◽  
Robert L. Mauck
Keyword(s):  
Type I Collagen ◽  
Cell Attachment ◽  
Type I ◽  
Primary Role ◽  
Fiber Reinforced ◽  
Collagen Scaffolds ◽  
Major Drawback ◽  
Aligned Arrays ◽  
Musculoskeletal Tissues ◽  
Nanofibrous Scaffolds

Collagen is a principal constituent of the extracellular matrix (ECM) and as such, defines the microenvironmental milieu in which cells reside. In fiber-reinforced musculoskeletal tissues, collagen fibers are highly organized and generate the direction-dependent mechanical properties critical to the function of these structures. Given its primary role, collagen is particularly attractive for tissue engineering (TE) applications where scaffolds are coupled with cells to repair or regenerate damaged tissues. One method for producing collagen-based scaffolds is through electrospinning. This technique yields nano- to micron-scale fibers similar in diameter to those of the native ECM. Towards engineering orthopaedic tissues, methods have been devised to electrospin fibers into aligned arrays that can recapitulate the anisotropy of fiber-reinforced tissues [1]. While a number of polymers have been electrospun, collagen-based scaffolds are especially promising as they provide a biomimetic interface for cell attachment [2]. Numerous investigators have electrospun collagen [3], one major drawback is their inherent instability in aqueous environments. To address this, various crosslinking agents including glutaraldehyde (GA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, and N-hydroxysuccinimide chemistries have been used, but these chemicals often prove cytotoxic or excessively laborious in application [4]. Even with crosslinking, dry as-formed nanofibrous collagen scaffolds with moduli greater than 50MPa diminish by 100-fold with rehydration [5].

scholarly journals Chitosan/ biphasic calcium phosphate nanofibrous scaffolds for bone tissue engineering

2020 ◽  
pp. 11-17
Author(s):  
Truong Le Bich Tram Truong
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Late Stage ◽  
Biphasic Calcium Phosphate ◽  
Cell Attachment ◽  
The Novel ◽  
Osteogenic Cells ◽  
Nanofibrous Scaffolds

In this article, chitosan/biphasic calcium phosphate (CS/BCP)nanofibers were prepared by electrospinning. From the culture of osteogenic cells, the biocompatibility of CS/BCP nanofibrous substrates was identified and increased by the photocrosslinking. The enhancement in cell attachment and proliferation was caused by the improvement in nanofibers’ mechanical properties. The biocompatibility to osteoblasts was also promoted with the content of BCP. The osteogenic differentiation in early, middle and late stage was encouraged by the addition of BCP on nanofibrous substrates. The CS/BCP nanofibers were highly specific to osteogenic cells, revealed by difficulties in the growth of non-osteogenic cells on this composite nanofibrous scaffold. The novel nanofibrous scaffolds showed great potential in the tissue engineering of bones.

Engineered Celery-Structured Electrospun Fibers Surface and Its Initial Cell Attachment Ability Effect

2020 ◽  
Vol 20 (7) ◽  
pp. 4336-4339
Author(s):  
Se Rim Jang ◽  
Chan Hee Park ◽  
Cheol Sang Kim
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Polymer Concentration ◽  
Initial Cell ◽  
Adhesion Ability ◽  
Topological Features ◽  
Nanofibrous Scaffolds ◽  
Initial Adhesion ◽  
A Cell ◽  
Attachment Ability

The fabrication of various types of scaffolds using electrospinning has been greatly researched for tissue engineering applications in recent times. The rapid initial cell adhesion in electrospun scaffolds helps in the rapid recovery of graft sites. The characteristics of nanofibrous scaffolds can be improved by modifying the topological features and surface of the nanofibers. Previous studies have shown that the scaffold structure is related to a cell attachment ability. In this study, we modified the surface of the fibers to mimic celery structure. It was confirmed that solvent evaporation and polymer concentration influenced the formation of the surface. This structural property can improve the initial adhesion ability of cells. Cellulose acetate solutions were prepared and tested in various concentrations (15 wt%, 20 wt%, and 30 wt%). Scanning electron microscopy (SEM), tensile test and cell experiments were performed to evaluate the physical properties and biocompatibility. The structure of the present nanofiber can be applied as a very effective scaffold and it is expected to have a positive effect in the tissue engineering field.

In Vitro Characterization of Thermal Behaviour and Bone Marrow Stromal Cell Attachment on Chitosan/Polycaprolactone (CS/PCL) Nanofibrous Scaffolds

2015 ◽  
Vol 7 (11) ◽  
pp. 2427-2435 ◽  
Author(s):  
Mohammed Fayez Al Rez ◽  
H. Fouad ◽  
Ezzedine Laourine ◽  
Martin Hild ◽  
Dilbar Aibibu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Thermal Behaviour ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cell Attachment ◽  
Marrow Stromal Cell ◽  
Nanofibrous Scaffolds

scholarly journals Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants

2015 ◽  
Vol 6 ◽  
pp. 254-262 ◽  
Author(s):  
Anna Maria Pappa ◽  
Varvara Karagkiozaki ◽  
Silke Krol ◽  
Spyros Kassavetis ◽  
Dimitris Konstantinou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Plasma Treatment ◽  
Photoelectron Spectroscopy ◽  
Oxygen Plasma ◽  
Cell Attachment ◽  
Oxygen Plasma Treatment ◽  
Depth Sensing ◽  
Force Microscopy ◽  
Nanofibrous Scaffolds ◽  
Cardiovascular Implants

Electrospun nanofibrous scaffolds have been extensively used in several biomedical applications for tissue engineering due to their morphological resemblance to the extracellular matrix (ECM). Especially, there is a need for the cardiovascular implants to exhibit a nanostructured surface that mimics the native endothelium in order to promote endothelialization and to reduce the complications of thrombosis and implant failure. Thus, we herein fabricated poly-ε-caprolactone (PCL) electrospun nanofibrous scaffolds, to serve as coatings for cardiovascular implants and guide tissue regeneration. Oxygen plasma treatment was applied in order to modify the surface chemistry of the scaffold and its effect on cell attachment and growth was evaluated. The conditions of the surface modification were properly adjusted in order to define those conditions of the treatment that result in surfaces favorable for cell growth, while maintaining morphological integrity and mechanical behavior. Goniometry (contact angle measurements), scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) measurements were used to evaluate the morphological and chemical changes induced by the plasma treatment. Moreover, depth-sensing nanoindentation was performed to study the resistance of the plasma-treated scaffolds to plastic deformation. Lastly, the cell studies indicated that all scaffolds were cytocompatible, with the plasma-treated ones expressing a more pronounced cell viability and adhesion. All the above findings demonstrate the great potential of these biomimetic tissue-engineering constructs as efficient coatings for enhanced compatibility of cardiovascular implants.

scholarly journals Electrospun Polyhydroxybutyrate and Poly(L-lactide-co-ε-caprolactone) Composites as Nanofibrous Scaffolds

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Donraporn Daranarong ◽  
Rodman T. H. Chan ◽  
Nico S. Wanandy ◽  
Robert Molloy ◽  
Winita Punyodom ◽  
...  
Keyword(s):  
Cell Cycle Progression ◽  
Cell Attachment ◽  
Fibre Diameter ◽  
Nerve Tissue ◽  
Mitochondrial Activity ◽  
Cycle Progression ◽  
Support Cell ◽  
Nanofibrous Scaffolds ◽  
Average Fibre ◽  
Fibrous Membranes

Electrospinning can produce nanofibrous scaffolds that mimic the architecture of the extracellular matrix and support cell attachment for tissue engineering applications. In this study, fibrous membranes of polyhydroxybutyrate (PHB) with various loadings of poly(L-lactide-co-ε-caprolactone) (PLCL) were successfully prepared by electrospinning. In comparison to PLCL scaffolds, PLCL blends with PHB exhibited more irregular fibre diameter distributions and higher average fibre diameters but there were no significant differences in pore size. PLCL/PHB scaffolds were more hydrophilic (<120°) with significantly reduced tensile strength (ca. 1 MPa) compared to PLCL scaffolds (150.9±2.8∘and5.8±0.5 MPa). Increasing PLCL loading in PHB/PLCL scaffolds significantly increased the extension at break, (4–6-fold). PLCL/PHB scaffolds supported greater adhesion and proliferation of olfactory ensheathing cells (OECs) than those exhibiting asynchronous growth on culture plates. Mitochondrial activity of cells cultivated on the electrospun blended membranes was enhanced compared to those grown on PLCL and PHB scaffolds (212, 179, and 153%, resp.). Analysis showed that PLCL/PHB nanofibrous membranes promoted cell cycle progression and reduced the onset of necrosis. Thus, electrospun PLCL/PHB composites promoted adhesion and proliferation of OECs when compared to their individual PLCL and PHB components suggesting potential in the repair and engineering of nerve tissue.

scholarly journals Design and Characterization of Maltose-Conjugated Polycaprolactone Nanofibrous Scaffolds for Uterine Tissue Engineering

2021 ◽  
Author(s):  
Srividya Hanuman ◽  
Manasa Nune
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Functional Characterization ◽  
Smooth Muscles ◽  
Uterine Tissue ◽  
Smooth Muscle Tissue ◽  
Nanofibrous Scaffolds ◽  
Uterine Anomalies ◽  
Major Treatment ◽  
Amine Groups

Abstract Purpose Uterine anomalies are prevalent in women, and the major treatment assisted to them is hysterectomy as donor availability is extremely low. To overcome this, engineering uterine myometrium smooth muscle tissue has become very important. Several studies have shown that polycaprolactone (PCL) nanofibers are very effective in engineering smooth muscles, as this type of scaffold has structural similarities to the extracellular matrices of the cells. Here, we hypothesize that by electrospinning PCL nanofibers, they form a suitable scaffold for uterine tissue engineering. Methods Polycaprolactone nanofibrous scaffolds were fabricated, and surface modification was performed following two step wet chemistry method. First step is aminolysis which introduces the primary amine groups on the PCL scaffolds following which maltose is conjugated on the scaffolds. This was confirmed by the ninhydrin assay for the presence of amine groups. This was followed by ELLA assay where the presence of maltose on the scaffold was quantified. Modified scaffolds were further characterized by scanning electron microscope (SEM), contact angle analysis and Fourier transform infrared spectroscopy (FTIR). MTT assay, live-dead assay and actin staining were performed on the maltose immobilization to study the improvement of the cell attachment and proliferation rates on the modified scaffolds. Results Human uterine fibroblast (HUF) cells displayed significant proliferation on the maltose-modified PCL scaffolds, and they also exhibited appropriate morphology indicating that these modified fibers are highly suitable for uterine cell growth. Conclusion Our results indicate that the fabricated maltose PCL (MPCL) scaffolds would be a potential biomaterial to treat uterine injuries and promote regeneration. Lay Summary and Future Work Uterine anomalies are prevalent in women, and the major treatment is hysterectomy as donor availability is extremely low. Over the past few years, considerable efforts have been directed towards uterine tissue regeneration. This study is to design a tissue engineered scaffold that could act as a human uterine myometrial patch. We propose to create uterine fibroblast-based synthetic scaffolds that act in a condition similar to the intrauterine microenvironment where the embryos are embedded in the uterine wall. For understanding of the efficiency of the myometrial patch, functional characterization will be performed to study the effects of estrogen and prostaglandins on myometrial activity of the designed patch. Results from these experiments will assist a deeper understanding of how to construct a total bioengineered uterus which can substitute the uterus transplantation procedure, which nonetheless is in its initial stages of development. Graphical Abstract

scholarly journals Development of a Bioactive Scaffold based on NGF Containing PCL/Chitosan Nanofibers for Nerve Regeneration

2021 ◽  
Vol 11 (5) ◽  
pp. 12606-12617
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Cell Attachment ◽  
Peripheral Nerve Regeneration ◽  
Supporting Cell ◽  
Promising Technique ◽  
Nanofibrous Scaffolds ◽  
Wide Range ◽  
Cell Processes ◽  
Surface Modified

Nanofibers are used in a wide range of applications, including scaffolds for tissue engineering. Electrospinning is a promising technique to fabricate nanofibrous scaffolds capable of supporting cell attachment and growth. Nanofibers with biomimetic alignment could also guide neural cell growth and orientation of cell processes necessary for peripheral nerve regeneration. This study fabricated aligned nanofibers of polycaprolactone/chitosan (PCL/CS) scaffolds and immobilized nerve growth factor (NGF) on them via dopamine coating mediated bonds to confer bioactivity to the scaffold and support attachment and growth of PC12 cells. The results showed that PCL/CS nanofibrous scaffolds revealed appropriate mechanical and surface properties. Cells remained viable on the scaffolds, and surface-modified aligned nanofibrous scaffolds interacted better with the cells, inducing neural morphology and orientation. Immobilization of NGF via polydopamine (PD) on nanofibers' surface proved to be a proper method to enhance PC12 cell attachment and proliferation. Thus, this construct could potentially be used as a scaffold for peripheral nerve regeneration.

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