Enhanced corneal permeation of coumarin-6 using nanoliposomes containing dipotassium glycyrrhizinate: in vitro mechanism and in vivo permeation evaluation

RSC Advances ◽  
2015 ◽  
Vol 5 (92) ◽  
pp. 75636-75647 ◽  
Author(s):  
Chuanlong Guo ◽  
Fenghua Cui ◽  
Mengshuang Li ◽  
Fengjie Li ◽  
Xianggen Wu
Keyword(s):  
Cellular Uptake ◽  
Liposomal Membrane ◽  
Corneal Permeation ◽  
Coumarin 6 ◽  
Corneal Penetration ◽  
Dipotassium Glycyrrhizinate

The fluidity of the liposomal membrane differently affected cellular uptake/internalization and in vivo corneal penetration of the nanoliposomes.

Corneal Penetration Behavior of β-Blocking Agents III: In Vitro–In Vivo Correlations

1983 ◽  
Vol 72 (11) ◽  
pp. 1279-1281 ◽  
Author(s):  
John L. Lach ◽  
Hong-Shian Huang ◽  
Ronald D. Schoenwald
Keyword(s):  
Blocking Agents ◽  
Penetration Behavior ◽  
Corneal Penetration

scholarly journals Zr-89 Immuno-PET Targeting Ectopic ATP Synthase Enables In-Vivo Imaging of Tumor Angiogenesis

2019 ◽  
Vol 20 (16) ◽  
pp. 3928
Author(s):  
Bok-Nam Park ◽  
Ga-Hee Kim ◽  
Seung-A Ko ◽  
Ga-Hee Shin ◽  
Su-Jin Lee ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cellular Uptake ◽  
Tumor Angiogenesis ◽  
Breast Cancer Cells ◽  
Xenograft Model ◽  
Tumor Uptake ◽  
Positron Emission ◽  
Pc3 Cells

In this study, we synthesized a Zr-89-labeled anti-adenosine triphosphate synthase monoclonal antibody (ATPS mAb) for applications in immuno-positron emission tomography (PET) and evaluated its feasibility for angiogenesis imaging. The cellular uptake of Zr-89 ATPS mAb was measured after treatment of cancer cell lines in vitro, and its biodistribution was evaluated at 4, 24 and 48 h in vivo in mice bearing xenografts. PET images were acquired at 4, 24, 48, and 96 h after Zr-89 ATPS mAb administration. Tumor angiogenesis was analyzed using anti-CD31 immunofluorescence staining. The cellular uptake of Zr-89 ATPS mAb increased over time in MDA-MB-231 breast cancer cells but did not increase in PC3 prostate cancer cells. The tumor uptake of Zr-89 ATPS mAb at 24 h was 9.4 ± 0.9% ID/g for MDA-Mb-231 cells and was 3.8 ± 0.6% ID/g for PC3 cells (p = 0.004). Zr-89 ATPS mAb uptake in MDA-MB-231 xenografts was inhibited by the administration of cold ATPS mAb (4.4 ± 0.5% ID/g, p = 0.011). Zr-89 ATPS mAb uptake could be visualized by PET for up to 96 h in MDA-MB-231 tumors. In contrast, there was no distinct tumor uptake detected by PET in the PC3 xenograft model. CD31-positive tumor vessels were abundant in MDA-MB-231 tumors, whereas they were scarcely detected in PC3 tumors. In conclusion, ATPS mAb was successfully labeled with Zr-89, which could be used for immuno-PET imaging targeting tumor angiogenesis.

Quercetin and fisetin enhanced the small intestine cellular uptake and plasma levels of epi-catechins in in vitro and in vivo models

2018 ◽  
Vol 9 (1) ◽  
pp. 234-242 ◽  
Author(s):  
Jin-Oh Chung ◽  
Seon-Bong Lee ◽  
Kang-Hyun Jeong ◽  
Ji-Hoon Song ◽  
Su-Kyung Kim ◽  
...  
Keyword(s):  
Small Intestine ◽  
Cellular Uptake ◽  
Plasma Levels ◽  
In Vivo Models ◽  
Strong Affinity

The catechol-containing flavonoids quercetin and fisetin could positively affect the absorption of catechins due to their strong affinity for COMT, which can methylate and cause the excretion of catechins.

Abstract 2013: Modulation of cellular uptake and increased therapeutic efficacy of the azacytidine-elaidic acid ester CP-4200 in vitro and in vivo

2011 ◽  
Author(s):  
Bodo Brueckner ◽  
Maria Rius ◽  
Maria Rivera Markelova ◽  
Iduna Fichtner ◽  
Petter-Arnt Hals ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Elaidic Acid ◽  
Therapeutic Efficacy ◽  
Acid Ester

scholarly journals Preparation, cellular uptake and angiogenic suppression of shikonin-containing liposomes in vitro and in vivo

2013 ◽  
Vol 33 (2) ◽  
Author(s):  
Hongmei Xia ◽  
Chengyi Tang ◽  
Heng Gui ◽  
Xiaoming Wang ◽  
Jinliang Qi ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Cellular Uptake ◽  
Mtt Assay ◽  
Chorioallantoic Membrane ◽  
The Self ◽  
Clinical Use ◽  
Phospholipid Membrane ◽  
Soybean Phospholipid

Shikonin has anticancer activity, but it has not yet been applied into clinical use. In the present study, shikonin was prepared using liposomes. We aimed to examine several aspects of sh-L (shikonin-containing liposomes): preparation, angiogenic suppression and cellular uptake through self-fluorescence. Sh-L were prepared using soybean phospholipid and cholesterol to form the membrane and shikonin was encapsulated into the phospholipid membrane. Three liposomes were prepared with shikonin. They had red fluorescence and were analysed using a flow cytometer. Angiogenic suppression of sh-L was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide], Transwell tests, chick CAM (chorioallantoic membrane) and Matrigel™ plug assay. MTT assay showed the median IC50 (inhibitory concentrations) as follows: shikonin, sh-L1 and sh-L2 were 4.99±0.23, 5.81±0.57 and 7.17±0.69 μM, respectively. The inhibition rates of migration were 53.58±7.05, 46.56±4.36 and 41.19±3.59% for 3.15 μM shikonin, sh-L1 and sh-L2, respectively. The results of CAM and Matrigel plug assay demonstrated that shikonin and sh-L can decrease neovascularization. Effect of shikonin was more obvious than sh-L at the same concentration. The results showed that sh-L decreased the toxicity, the rate of inhibition of migration and angiogenic suppression. The cellular uptake of the sh-L could be pictured because of the self-fluorescence. The self-fluorescence will be useful for conducting further research. Sh-L might be an excellent preparation for future clinical application to cancer patients.

Radioisotope Co-57 incorporated layered double hydroxide nanoparticles as a cancer imaging agent

RSC Advances ◽  
2016 ◽  
Vol 6 (54) ◽  
pp. 48415-48419 ◽  
Author(s):  
Tae-Hyun Kim ◽  
Jun Young Lee ◽  
Min-Kyu Kim ◽  
Jeong Hoon Park ◽  
Jae-Min Oh
Keyword(s):  
Layered Double Hydroxide ◽  
Cellular Uptake ◽  
Tumor Targeting ◽  
Cancer Imaging ◽  
Imaging Agent ◽  
Isomorphous Substitution ◽  
Double Hydroxide ◽  
Layered Double Hydroxide Nanoparticles

Radioisotope Co-57 substituted LDH were successfully prepared by isomorphous substitution and showed high in vitro cellular uptake and tumor targeting in vivo biodistribution.

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