A novel strategy for screening new natural products by a combination of reversed-phase liquid chromatography fractionation and 13C NMR pattern recognition: the discovery of new anti-cancer flavone dimers from Dysosma versipellis (Hance)

RSC Advances ◽  
2015 ◽  
Vol 5 (95) ◽  
pp. 77553-77564 ◽  
Author(s):  
Zhi Yang ◽  
Youqian Wu ◽  
Hui Zhou ◽  
Xiaoji Cao ◽  
Xinhang Jiang ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
Natural Products ◽  
Liquid Chromatography ◽  
Reversed Phase ◽  
Screening Strategy ◽  
Reversed Phase Liquid Chromatography ◽  
Dysosma Versipellis ◽  
Anti Cancer ◽  
Phase Liquid ◽  
Novel Strategy

A new screening strategy for the discovery of new natural products by a combination of reversed-phase liquid chromatography (RPLC) and 13C NMR pattern recognition.

scholarly journals Untargeted Metabolomics Studies on Drug-Incubated Phragmites australis Profiles

Metabolites ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 2
Author(s):  
Rofida Wahman ◽  
Andres Sauvêtre ◽  
Peter Schröder ◽  
Stefan Moser ◽  
Thomas Letzel
Keyword(s):  
Liquid Chromatography ◽  
Phragmites Australis ◽  
Transformation Products ◽  
Reversed Phase ◽  
Screening Strategy ◽  
Hydrophilic Interaction ◽  
Reversed Phase Liquid Chromatography ◽  
Plant Metabolites ◽  
Huge Number ◽  
Phase Liquid

Plants produce a huge number of functionally and chemically different natural products that play an important role in linking the plant with the adjacent environment. Plants can also absorb and transform external organic compounds (xenobiotics). Currently there are only a few studies concerning the effects of xenobiotics and their transformation products on plant metabolites using a mass spectrometric untargeted screening strategy. This study was designed to investigate the changes of the Phragmites australis metabolome following/after diclofenac or carbamazepine incubation, using a serial coupling of reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) combined with accurate high-resolution time-of-flight mass spectrometer (TOF-MS). An untargeted screening strategy of metabolic fingerprints was developed to purposefully compare samples from differently treated P. australis plants, revealing that P. australis responded to each drug differently. When solvents with significantly different polarities were used, the metabolic profiles of P. australis were found to change significantly. For instance, the production of polyphenols (such as quercetin) in the plant increased after diclofenac incubation. Moreover, the pathway of unsaturated organic acids became more prominent, eventually as a reaction to protect the cells against reactive oxygen species (ROS). Hence, P. australis exhibited an adaptive mechanism to cope with each drug. Consequently, the untargeted screening approach is essential for understanding the complex response of plants to xenobiotics.

Study on Retention Equation for Protein in Reversed Phase Liquid Chromatography

2013 ◽  
Vol 41 (2) ◽  
pp. 181
Author(s):  
Ling DING ◽  
Jun DONG ◽  
Yuan-Sheng XIAO ◽  
Xiu-Li ZHANG ◽  
Xing-Ya XUE ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Retention Equation ◽  
Phase Liquid
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