Magnetic chemiluminescent immunoassay for human C-reactive protein on the centrifugal microfluidics platform

RSC Advances ◽  
2015 ◽  
Vol 5 (76) ◽  
pp. 61906-61912 ◽  
Author(s):  
G. Czilwik ◽  
S. K. Vashist ◽  
V. Klein ◽  
A. Buderer ◽  
G. Roth ◽  
...  
Keyword(s):  
Centrifugal Microfluidics ◽  
Magnetic Actuation ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Chemiluminescent Immunoassay ◽  
Microfluidics Platform

Schematic of the LabDisk-based hCRP MCIA. The antibody-coated dynabeads are sequentially transported through the immunoassay buffers by magnetic actuation. Finally the chemiluminescence signal is acquired from a detection cavity.

scholarly journals Siphon-Controlled Automation on a Lab-on-a-Disc Using Event-Triggered Dissolvable Film Valves

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 73
Author(s):  
Brian D. Henderson ◽  
David J. Kinahan ◽  
Jeanne Rio ◽  
Rohit Mishra ◽  
Damien King ◽  
...  
Keyword(s):  
Risk Factor ◽  
Limit Of Detection ◽  
C Reactive Protein ◽  
Cvd Risk ◽  
Low Resource Settings ◽  
Low Resource ◽  
Reactive Protein ◽  
Limit Of Quantitation ◽  
Chemiluminescent Immunoassay ◽  
Event Triggered

Within microfluidic technologies, the centrifugal microfluidic “Lab-on-a-Disc” (LoaD) platform offers great potential for use at the PoC and in low-resource settings due to its robustness and the ability to port and miniaturize ‘wet bench’ laboratory protocols. We present the combination of ‘event-triggered dissolvable film valves’ with a centrifugo-pneumatic siphon structure to enable control and timing, through changes in disc spin-speed, of the release and incubations of eight samples/reagents/wash buffers. Based on these microfluidic techniques, we integrated and automated a chemiluminescent immunoassay for detection of the CVD risk factor marker C-reactive protein displaying a limit of detection (LOD) of 44.87 ng mL−1 and limit of quantitation (LoQ) of 135.87 ng mL−1.

908: Preoperative C-Reactive Protein Elevation as a Predictor of Localized Renal Cell Carcinoma

2007 ◽  
Vol 177 (4S) ◽  
pp. 301-301
Author(s):  
Yasumasa Iimura ◽  
Kazutaka Saito ◽  
Minato Yokoyama ◽  
Hitoshi Masuda ◽  
Tsuyoshi Kobayashi ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Localized Renal Cell Carcinoma
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