A simple and rapid approach for testing enantiopurity of hydroxy acids and their derivatives using 1H NMR spectroscopy

RSC Advances ◽  
2015 ◽  
Vol 5 (82) ◽  
pp. 67277-67283 ◽  
Author(s):  
Sandeep Kumar Mishra ◽  
N. Suryaprakash
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Accurate Determination ◽  
Hydroxy Acids ◽  
Optically Pure

A rapid and the simple chiral derivatizing protocol involving the coupling of 2-formylphenylboronic acid and an optically pure [1,1-binaphthalene]-2,2-diamine is introduced for the accurate determination of the enantiopurity of hydroxy acids and their derivatives.

Accurate Determination of Reference Materials and Natural Isolates by Means of Quantitative 1H NMR Spectroscopy

2014 ◽  
Vol 62 (12) ◽  
pp. 2506-2515 ◽  
Author(s):  
Oliver Frank ◽  
Johanna Karoline Kreissl ◽  
Andreas Daschner ◽  
Thomas Hofmann
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
Reference Materials ◽  
1H Nmr Spectroscopy ◽  
Accurate Determination ◽  
Natural Isolates

scholarly journals pKa Determination of a Histidine Residue in a Short Peptide Using Raman Spectroscopy

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 405 ◽  
Author(s):  
Brett H. Pogostin ◽  
Anders Malmendal ◽  
Casey H. Londergan ◽  
Karin S. Åkerfeldt
Keyword(s):  
Raman Spectroscopy ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Peptide Sequence ◽  
Short Peptide ◽  
Dependent Behavior ◽  
Pka Determination ◽  
Key Functional Groups

Determining the pKa of key functional groups is critical to understanding the pH-dependent behavior of biological proteins and peptide-based biomaterials. Traditionally, 1H NMR spectroscopy has been used to determine the pKa of amino acids; however, for larger molecules and aggregating systems, this method can be practically impossible. Previous studies concluded that the C-D stretches in Raman are a useful alternative for determining the pKa of histidine residues. In this study, we report on the Raman application of the C2-D probe on histidine’s imidazole side chain to determining the pKa of histidine in a short peptide sequence. The pKa of the tripeptide was found via difference Raman spectroscopy to be 6.82, and this value was independently confirmed via 1H NMR spectroscopy on the same peptide. The C2-D probe was also compared to other Raman reporters of the protonation state of histidine and was determined to be more sensitive and reliable than other protonation-dependent signals. The C2-D Raman probe expands the tool box available to chemists interested in directly interrogating the pKa’s of histidine-containing peptide and protein systems.

1H NMR Spectroscopy for Determination of the Geographical Origin of Hazelnuts

2018 ◽  
Vol 66 (44) ◽  
pp. 11873-11879 ◽  
Author(s):  
René Bachmann ◽  
Sven Klockmann ◽  
Johanna Haerdter ◽  
Markus Fischer ◽  
Thomas Hackl
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Geographical Origin

scholarly journals Composition Determination of Low-Pressure Gas-Phase Mixtures by 1H NMR Spectroscopy

2019 ◽  
Vol 91 (7) ◽  
pp. 4429-4435 ◽  
Author(s):  
Christopher L. Suiter ◽  
Mark O. McLinden ◽  
Thomas J. Bruno ◽  
Jason A. Widegren
Keyword(s):  
Nmr Spectroscopy ◽  
Gas Phase ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Low Pressure ◽  
Composition Determination
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