Fabricating a morphology tunable patterned bio-inspired polydopamine film directly via microcontact printing

RSC Advances ◽  
2015 ◽  
Vol 5 (75) ◽  
pp. 60990-60992 ◽  
Author(s):  
Yanshan Hou ◽  
Peng Xiao ◽  
Jiawei Zhang ◽  
Meiwen Peng ◽  
Wei Lu ◽  
...  
Keyword(s):  
Microcontact Printing ◽  
Simple Strategy ◽  
Tunable Morphology

A robust and simple strategy of microcontact printing is provided to fabricate micro-patterned polydopamine (PDA) films with tunable morphology achieved by altering the concentration of PDA “ink” solution.

scholarly journals Indirect Microcontact Printing to Create Functional Patterns of Physisorbed Antibodies

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 3163 ◽  
Author(s):  
Augusto Juste-Dolz ◽  
Miquel Avella-Oliver ◽  
Rosa Puchades ◽  
Angel Maquieira
Keyword(s):  
Biological Activity ◽  
Bovine Serum Albumin ◽  
Conformational Changes ◽  
Immunoglobulin E ◽  
Microcontact Printing ◽  
Label Free ◽  
Simple Strategy ◽  
Solid Substrates ◽  
Functional Patterns ◽  
Nanostructured Patterns

Microcontact printing (µCP) is a practical and versatile approach to create nanostructured patterns of biomolecular probes, but it involves conformational changes on the patterned bioreceptors that often lead to a loss on the biological activity of the resulting structures. Herein we introduce indirect µCP to create functional patterns of bioreceptors on solid substrates. This is a simple strategy that relies on physisorbing biomolecular probes of interest in the nanostructured gaps that result after patterning backfilling agents by standard µCP. This study presents the approach, assesses bovine serum albumin as backfilling agent for indirect µCP on different materials, reports the limitations of standard µCP on the functionality of patterned antibodies, and demonstrates the capabilities of indirect µCP to solve this issue. Bioreceptors were herein structured as diffractive gratings and used to measure biorecognition events in label-free conditions. Besides, as a preliminary approach towards sensing biomarkers, this work also reports the implementation of indirect µCP in an immunoassay to detect human immunoglobulin E.

Co-assembly of sugar-based amphiphilic block polymers to achieve nanoparticles with tunable morphology, size, surface charge, and acid-responsive behavior

2018 ◽  
Vol 2 (12) ◽  
pp. 2230-2238 ◽  
Author(s):  
Yen-Nan Lin ◽  
Lu Su ◽  
Justin Smolen ◽  
Richen Li ◽  
Yue Song ◽  
...  
Keyword(s):  
Surface Charge ◽  
Block Polymers ◽  
Simple Strategy ◽  
Nanoparticle Morphology ◽  
Responsive Behavior ◽  
Amphiphilic Block Polymers ◽  
Tunable Morphology

Co-assembly of glucose-based polymers is demonstrated as a simple strategy to control nanoparticle morphology, size, surface charge, and acid-responsive properties.

A simple strategy for fabricating honeycomb patterns on commercially available polymer film under a wide humidity range

2020 ◽  
Vol 35 (23-24) ◽  
pp. 3210-3221
Author(s):  
Kai Huang ◽  
Qi Cheng ◽  
Honglei Zhang ◽  
Ligang Lin ◽  
Qiying Wang
Keyword(s):  
Polymer Film ◽  
Simple Strategy ◽  
Humidity Range

Abstract

Hierarchy of π-Stacking Determines the Conformational Preference of Bis-Squaraines

2020 ◽  
Author(s):  
Abhishek Singh ◽  
Reman K. Singh ◽  
G Naresh Patwari
Keyword(s):  
Hydrogen Bonding ◽  
Rational Design ◽  
Biological Molecules ◽  
Conformational Space ◽  
X Ray Crystallography ◽  
Simple Strategy ◽  
Induced Folding ◽  
Π Stacking ◽  
Varying Length ◽  
Dynamics Simulations

The rational design of conformationally controlled foldable modules can lead to a deeper insight into the conformational space of complex biological molecules where non-covalent interactions such as hydrogen bonding and π-stacking are known to play a pivotal role. Squaramides are known to have excellent hydrogen bonding capabilities and hence, are ideal molecules for designing foldable modules that can mimic the secondary structures of bio-molecules. The π-stacking induced folding of bis-squaraines tethered using aliphatic primary and secondary-diamine linkers of varying length is explored with a simple strategy of invoking small perturbations involving the length linkers and degree of substitution. Solution phase NMR investigations in combination with molecular dynamics simulations suggest that bis-squaraines predominantly exist as extended conformations. Structures elucidated by X-ray crystallography confirmed a variety of folded and extended secondary conformations including hairpin turns and 𝛽-sheets which are determined by the hierarchy of π-stacking relative to N–H···O hydrogen bonds.

Hierarchy of π-Stacking Determines the Conformational Preference of Bis-Squaraines

2020 ◽  
Author(s):  
Abhishek Singh ◽  
Reman K. Singh ◽  
G Naresh Patwari
Keyword(s):  
Hydrogen Bonding ◽  
Rational Design ◽  
Biological Molecules ◽  
Conformational Space ◽  
X Ray Crystallography ◽  
Simple Strategy ◽  
Induced Folding ◽  
Π Stacking ◽  
Varying Length ◽  
Dynamics Simulations

The rational design of conformationally controlled foldable modules can lead to a deeper insight into the conformational space of complex biological molecules where non-covalent interactions such as hydrogen bonding and π-stacking are known to play a pivotal role. Squaramides are known to have excellent hydrogen bonding capabilities and hence, are ideal molecules for designing foldable modules that can mimic the secondary structures of bio-molecules. The π-stacking induced folding of bis-squaraines tethered using aliphatic primary and secondary-diamine linkers of varying length is explored with a simple strategy of invoking small perturbations involving the length linkers and degree of substitution. Solution phase NMR investigations in combination with molecular dynamics simulations suggest that bis-squaraines predominantly exist as extended conformations. Structures elucidated by X-ray crystallography confirmed a variety of folded and extended secondary conformations including hairpin turns and 𝛽-sheets which are determined by the hierarchy of π-stacking relative to N–H···O hydrogen bonds.

Revealing changes in curcumin bioavailability using vitamin C as an enhancer by HPLC-MS/MS

2019 ◽  
Vol 16 ◽  
Author(s):  
Xufen Dai ◽  
Jiaxue Hao ◽  
Ying Feng ◽  
Jing Wang ◽  
Qiannan Li ◽  
...  
Keyword(s):  
Vitamin C ◽  
High Performance ◽  
Internal Standard ◽  
Fold Increase ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Solution Ph ◽  
Esi Ms ◽  
Simple Strategy ◽  
Isolated Compound

Background: Curcumin (CUR), a natural isolated compound from turmeric, has been the promising star in fighting many diseases but the broad application of curcumin has been limited ascribed to low bioavailability. Objective: The aim of this study is to pursue the enhancement of curcumin bioavailability through co-administration of vitamin C. Methods: Such purpose was achieved through the analysis of curcumin pharmacokinetics by high performance liquid chromatography coupled with electrospray ionization - tandem mass spectrometry (HPLC - ESI - MS/MS). The plasma was separated on a C18 reverse phase column using acetonitrile and ammonium formate solution (pH 6.5; 2.0 mM) at 0.8 mL/min. MS/MS detection was carried out in negative mode using mass patterns of m/z 367.0 > 216.7 for curcumin and m/z 265.2 > 223.9 for internal standard (honokiol). Results: Successful application of the proposed method in the pharmacokinetic study presented clear changes in key pharmacokinetic parameters including the growth of AUC (0-t) up to 2.4 times, 2.2-fold increase of Cmax, 2.2-fold loss of CL, and 1.5-fold diminishment of t1/2. Conclusion: We developed an HPLC-ESI-MS/MS method for determination of curcumin in rat plasma and validated the improvement of bioavailability of curcumin through co-administration of vitamin C. We reasoned these changes to the inhibition of lipid peroxidation induced by the use of vitamin C. Such a simple strategy is possible to become an alternative for enhancing curcumin efficiency in practice.

A simple strategy increases mothers’ knowledge of breastfeeding and improves the breastfeeding rates

1998 ◽  
Vol 74 (5) ◽  
pp. 368-76 ◽  
Author(s):  
Lulie R.O. Susin ◽  
Elsa R. J. Giugliani ◽  
Suzane C. Kummer ◽  
Marileide Maciel ◽  
Ana C. W. Benjamin ◽  
...  
Keyword(s):  
Simple Strategy ◽  
Breastfeeding Rates

A simple strategy based on photobiotin irradiation for the photoelectrochemical immobilization of proteins on electrode surfaces

2006 ◽  
Vol 26 (2-3) ◽  
pp. 436-441 ◽  
Author(s):  
Serge Cosnier ◽  
Carmen Molins ◽  
Christine Mousty ◽  
Bruno Galland ◽  
Arielle Lepellec
Keyword(s):  
Electrode Surfaces ◽  
Simple Strategy

Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing

Biomaterials ◽  
2009 ◽  
Vol 30 (29) ◽  
pp. 5427-5432 ◽  
Author(s):  
Imen Elloumi Hannachi ◽  
Kazuyoshi Itoga ◽  
Yoshikazu Kumashiro ◽  
Jun Kobayashi ◽  
Masayuki Yamato ◽  
...  
Keyword(s):  
Microcontact Printing ◽  
Cell Sheets ◽  
Cultured Cell

scholarly journals A Novel Quinoxaline-Rhodamine Conjugate for a Simple and Efficient Detection of Hydrogen Sulphate Ion

Compounds ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 29-40
Author(s):  
Sutapa Sahu ◽  
Yeasin Sikdar ◽  
Riya Bag ◽  
Dilip K. Maiti ◽  
José P. Cerón-Carrasco ◽  
...  
Keyword(s):  
Solid Phase ◽  
Detection Limits ◽  
Hydrogen Sulfate ◽  
Absorption And Emission ◽  
Conjugate System ◽  
Colorimetric Chemosensor ◽  
Simple Strategy ◽  
Efficient Detection ◽  
Hydrogen Sulphate

This work presents the development of a quinoxaline and rhodamine conjugate system that acts as a colorimetric chemosensor for hydrogen sulfate (HSO4−) ions in methanol media. This sensor has been characterized both theoretically and experimentally. The detection limits for HSO4− are small as 0.71 µM and 3.8 µM for the absorption and emission experiments, respectively. The effectiveness of the probe in recognizing HSO4− both in gel and solid phase is evaluated as well. Thus, this works presents a simple strategy to detect the environmental HSO4− pollutant event at tiny concentrations.

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