TNPs as a novel fluorescent sensor for the selective recognition of fast green FCF: a spectrofluorimetric approach

RSC Advances ◽  
2015 ◽  
Vol 5 (85) ◽  
pp. 69371-69377 ◽  
Author(s):  
Dattatray K. Dalavi ◽  
Avinash Kamble ◽  
Dhanaji P. Bhopate ◽  
Prasad G. Mahajan ◽  
Govind B. Kolekar ◽  
...  
Keyword(s):  
Ground State ◽  
Fluorescent Sensor ◽  
Selective Recognition ◽  
Fast Green ◽  
Fast Green Fcf

The fast green FCF dye adsorbed over the surface of the CTAB stabilized tetracene nanoparticles (TNPs) forms a stable, non-fluorescent ground state complex and quenches fluorescence of nanoparticle sensor.

scholarly journals A water-soluble fluorescent chemosensor based on Asp functionalized naphthalimide for successive detection Fe3+ and H2PO4−

2018 ◽  
Vol 96 (4) ◽  
pp. 363-370 ◽  
Author(s):  
You-Ming Zhang ◽  
Xiao-Peng Chen ◽  
Guo-Yan Liang ◽  
Kai-Peng Zhong ◽  
Hong Yao ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Metal Ions ◽  
Aspartic Acid ◽  
High Selectivity ◽  
Fluorescent Sensor ◽  
Water Solution ◽  
Water Soluble ◽  
Selective Recognition ◽  
Actual Usage ◽  
Quenching Process

The selective recognition of target ions in water is very important and the development of novel water-soluble chemosensor is still an intriguing challenge. Herein, a novel water-soluble fluorescent sensor based on aspartic acid (Asp) functionalized 1,8-naphthalimide derivative (Asp-NI) has been designed and synthesized. The sensor Asp-NI could dissolve in water and successively detect Fe3+ and H2PO4− in water solution with high selectivity and sensitivity. The detection limits are 4.97 × 10−7 mol/L for Fe3+ and 5.27 × 10−6 mol/L for H2PO4−. Other coexistent competitive metal ions (Hg2+, Ag+, Ca2+, Cu2+, Co2+, Ni2+, Cd2+, Pb2+, Zn2+, Cr3+, and Mg2+) showed no interference in the Fe3+ detection process. The sensor Asp-NI could act as a Fe3+ and H2PO4− controlled “On–Off–On” fluorescent switch. More interestingly, the Fe3+ induced fluorescence quenching process could be totally reversed by the addition of H2PO4−, this “On–Off–On” switching process could be repeated several times with little fluorescence loss. Notably, the actual usage of sensor Asp-NI was further demonstrated by test kits.

Use of Fast Green FCF with Tryptic Soy Agar for Aerobic Plate Count by the Hydrophobic Grid Membrane Filter

1986 ◽  
Vol 49 (4) ◽  
pp. 278-279 ◽  
Author(s):  
PHYLLIS ENTIS ◽  
PETER BOLESZCZUK
Keyword(s):  
Membrane Filter ◽  
Food Samples ◽  
T Test ◽  
Plate Count ◽  
Paired Data ◽  
Plate Method ◽  
Fast Green ◽  
Product Categories ◽  
Aerobic Plate Count ◽  
Fast Green Fcf

A hydrophobic grid membrane filter (HGMF) method for aerobic plate count using Tryptic Soy Agar with fast green FCF was evaluated against a conventional pour plate method on 250 food samples, representing 25 product categories. The HGMF method yielded counts equivalent to or significantly higher than the pour plate method for 24 of the 25 product categories (t-test for paired data).

scholarly journals The antimicrobial activity of cationic proteins isolated from the cells in bulk milk samples

1971 ◽  
Vol 69 (1) ◽  
pp. 61-68 ◽  
Author(s):  
K. G. Hibbitt ◽  
J. Brownlie ◽  
C. B. Cole
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Agalactiae ◽  
Protein Fractions ◽  
Fast Green ◽  
Cationic Proteins ◽  
Disk Electrophoresis ◽  
Milk Samples ◽  
Bulk Milk ◽  
Micrococcus Lysodeikticus ◽  
Fast Green Fcf

SUMMARYCationic proteins isolated from the cells in bulk milk samples were shown to inhibit the growth of two pathogenic strains of staphylococci and alsoStreptococcus agalactiaeS 13. Polyacrylamide gel disk electrophoresis studies on these proteins revealed the presence of at least 9 components some of which had isoelectric pH's between 7·0 and 9·0. Trace amounts of the isolated protein had isoelectric pH's greater than 9·0. Staphylococci incubated with milk-cell cationic proteins absorbed the protein, thereby allowing the organism to be stained with the anionic dye Fast Green FCF. Protein-treated staphylococci in isotonic solutions autoagglutinated. This autoagglutination was more marked in hypo- and hypertonic solutions. Lysozyme was not demonstrated in the isolated protein fractions in assays involving incubation withMicrococcus lysodeikticusfor 90 min. The antimicrobial activity of the cationic proteins isolated from the bulk milk samples was not destroyed after heating to temperatures up to 70° C for 30 min., whereas at higher temperatures the activity diminished and was almost completely lost at 100° C.

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