Analyzing the effects of protecting osmolytes on solute–water interactions by solvatochromic comparison method: II. Globular proteins

RSC Advances ◽  
2015 ◽  
Vol 5 (73) ◽  
pp. 59780-59791 ◽  
Author(s):  
Luisa A. Ferreira ◽  
Xiao Fan ◽  
Pedro P. Madeira ◽  
Lukasz Kurgan ◽  
Vladimir N. Uversky ◽  
...  
Keyword(s):  
Phosphate Buffer ◽  
Potassium Phosphate ◽  
Globular Proteins ◽  
Comparison Method ◽  
Potassium Phosphate Buffer ◽  
Two Phase ◽  
Sodium Potassium ◽  
Phase Systems ◽  
Aqueous Dextran ◽  
Solute Water

Partitioning of 11 globular proteins was examined in aqueous dextran–PEG–sodium/potassium phosphate buffer (0.01 M K/NaPB, pH 7.4) two-phase systems (ATPSs) containing 0.5 M sorbitol.

Novel aqueous two-phase systems based on tetrahydrofuran and potassium phosphate buffer for purification of lipase

2015 ◽  
Vol 50 (9) ◽  
pp. 1459-1467 ◽  
Author(s):  
Ranyere L. Souza ◽  
Rafaella A. Lima ◽  
João A.P. Coutinho ◽  
Cleide M.F. Soares ◽  
Álvaro S. Lima
Keyword(s):  
Phosphate Buffer ◽  
Potassium Phosphate ◽  
Potassium Phosphate Buffer ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Porcine pancreatic lipase partition in potassium phosphate–polyethylene glycol aqueous two-phase systems

2007 ◽  
Vol 859 (2) ◽  
pp. 222-228 ◽  
Author(s):  
Georgina Bassani ◽  
Beatriz Farruggia ◽  
Bibiana Nerli ◽  
Diana Romanini ◽  
Guillermo Picó
Keyword(s):  
Polyethylene Glycol ◽  
Pancreatic Lipase ◽  
Potassium Phosphate ◽  
Porcine Pancreatic Lipase ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Protecting ultra- and hyperhydrophilic implant surfaces in dry state from loss of wettability

2016 ◽  
Vol 2 (1) ◽  
pp. 557-560 ◽  
Author(s):  
Steffen Lüers ◽  
Markus Laub ◽  
Herbert P. Jennissen
Keyword(s):  
Dental Implants ◽  
Phosphate Buffer ◽  
Potassium Phosphate ◽  
Optimal System ◽  
Potassium Phosphate Buffer ◽  
Long Time ◽  
Optical Appearance

AbstractUltrahydrophilic titanium miniplates with sandblasted and acid etched (SLA) surfaces were protected from loss of hydrophilicity by an exsiccation layer of salt and stored in a dry state. Various salts in different concentrations were tested in respect to their conservation capacity and optical appearance. Potassium phosphate buffer in a specified composition appeared to be optimal. This optimal system was applied in a long time storage experiment showing no loss of hydrophilicity over years. It was also transferred with success to hyperhydrophilic dental implants.

scholarly journals An Elution Procedure for Visualization of Adult Hemoglobins in Human Blood Smears

Blood ◽  
1974 ◽  
Vol 43 (2) ◽  
pp. 239-242 ◽  
Author(s):  
David Kabat
Keyword(s):  
Cord Blood ◽  
Human Blood ◽  
Phosphate Buffer ◽  
Newborn Infants ◽  
Potassium Phosphate ◽  
Fetal Hemoglobin ◽  
Potassium Phosphate Buffer ◽  
Blood Smears

Abstract A procedure is described for visualization of normal and mutant adult hemoglobins in human blood smears. After extraction of blood smears with a concentrated potassium phosphate buffer (2.76 M, pH 7.2), erythrocytes that had adult hemoglobins stained bright red with erythrosin, whereas cells that had only fetal hemoglobin appeared as clear ghosts. Analyses of cord blood from newborn infants indicate that, although most erythrocytes contain only Hb F and a few contain only Hb A, many contain both hemoglobins A and F.

Repair of salt tolerance and recovery of lost D-alanine and magnesium following sublethal heating of Staphylococcus aureus are independent events

1981 ◽  
Vol 27 (6) ◽  
pp. 627-632 ◽  
Author(s):  
A. Hurst ◽  
A. Hughes
Keyword(s):  
Staphylococcus Aureus ◽  
Salt Tolerance ◽  
Phosphate Buffer ◽  
Synthetic Medium ◽  
Potassium Phosphate ◽  
Complex Media ◽  
Rich Medium ◽  
Potassium Phosphate Buffer ◽  
Heat Damage ◽  
Independent Events

Sublethal heating of Staphylococcus aureus S6 in potassium phosphate buffer caused loss of salt tolerance, D-alanine, and magnesium. During incubation in rich complex media all three of the damaged sites were repaired. Repair occurred more slowly but went to completion in a dilute synthetic medium (DSM), free of D-ala. DSM plus penicillin or D-cycloserine allowed repair of salt tolerance but recovery of normal levels of D-ala or Mg was prevented. When DSM-repaired cells were cultured into fresh rich medium they grew rapidly after a short lag. Cells which had acquired their salt tolerance in DSM plus cycloserine and were D-ala and Mg deficient grew slowly and had a lag of 3 h. We suggest that heat damage has two separate primary targets in S. aureus cells: the membrane, which is manifested by loss of salt tolerance, and a second site, possibly teichoic acids, manifested by loss of D-ala and Mg.

scholarly journals Purification and general properties of δ-aminolaevulate dehydratase from Nicotiana tabacum L.

1969 ◽  
Vol 114 (2) ◽  
pp. 331-337 ◽  
Author(s):  
A. S. Shetty ◽  
G. W. Miller
Keyword(s):  
Activation Energy ◽  
Nicotiana Tabacum ◽  
Phosphate Buffer ◽  
Potassium Phosphate ◽  
Optimum Temperature ◽  
Thiol Groups ◽  
Potassium Phosphate Buffer ◽  
Tobacco Leaves ◽  
General Properties ◽  
Nicotiana Tabacum L

1. δ-Aminolaevulate dehydratase (EC 4.2.1.24) was purified 80-fold from tobacco leaves and its properties were studied. 2. The enzyme had optimum pH7·4 in potassium phosphate buffer, Km6·25×10−4m at 37° and pH7·4, optimum temperature 45° and an activation energy of 11100 cal./mole. 3. The enzyme lost activity when prepared in the absence of cysteine, and this activity was only partly restored by the later addition of thiols. Reagents for thiol groups inactivated the enzyme. 4. Mg2+ was essential for activity, and EDTA and Fe2+ were inhibitory; Mn2+ was an activator or an inhibitor depending on the concentration.

Reduction of 7-ketolithocholic acid by human liver enzyme preparations in vitro

1989 ◽  
Vol 256 (1) ◽  
pp. G67-G71
Author(s):  
Y. Amuro ◽  
W. Yamade ◽  
K. Kudo ◽  
T. Yamamoto ◽  
T. Hada ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Liver Enzyme ◽  
Chenodeoxycholic Acid ◽  
Human Liver ◽  
Potassium Phosphate ◽  
Gas Chromatography Mass Spectrometry ◽  
Potassium Phosphate Buffer ◽  
Sodium Potassium ◽  
Enzyme Preparations

The formation of chenodeoxycholic and ursodeoxycholic acids from 7-ketolithocholic acid by human liver preparations was examined in vitro. Liver preparations were incubated with 7-ketolithocholic acid at pH 5.5 in a sodium-potassium-phosphate buffer containing NADPH or NADH. The products formed were analyzed by gas chromatography and gas chromatography-mass spectrometry. Results showed that chenodeoxycholic and ursodeoxycholic acids could be formed from 7-ketolithocholic acid by human liver enzyme(s). The enzyme(s) required NADPH but not NADH as coenzyme and was localized largely in the microsomes. The conjugated 7-ketolithocholic acid, especially the taurine conjugated, was predominantly reduced to chenodeoxycholic acid, whereas the unconjugated 7-ketolithocholic acid was not reduced well to either chenodeoxycholic acid or ursodeoxycholic acid. Thus the reduction of 7-ketolithocholic acid by human liver enzyme(s) was found to be dependent on whether the substrate was conjugated or not.

Factors that Influence Germination and Mycoherbicidal Activity ofAlternaria cassiae

1991 ◽  
Vol 5 (1) ◽  
pp. 82-86 ◽  
Author(s):  
Donald J. Daigle ◽  
Peter J. Cotty
Keyword(s):  
Relative Humidity ◽  
Phosphate Buffer ◽  
Potassium Phosphate ◽  
Tween 80 ◽  
Growth Chamber ◽  
Potassium Phosphate Buffer ◽  
Potato Dextrose Broth ◽  
Leaf Stage ◽  
True Leaf

The influences of pH, surfactants, and nutrients on germination were investigated to develop a basis for improvement ofAlternaria cassiaemycoherbicide formulations. In vitro results indicated that a formulation with a pH of approximately 6.5 containing 0.1 to 1% Tween 80, 0.02 M potassium phosphate buffer, and 1% dehydrated potato dextrose broth best promoted germination. Sicklepod plants at the 2 to 3 true-leaf stage were sprayed with test solutions, incubated in the dark at 100% relative humidity (28 C) for 6 h, and placed in a growth chamber maintained at 30 C. Assessment of the plants after 2 d indicated that the ability of the formulation components to induce germination ofAlternaria cassiaein vitro corresponded well with their ability to improve infection of sicklepod seedlings.

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