Optimization of ultrasound-assisted extraction (UAE) of phenolic compounds from Crataegus pinnatifida leaves and evaluation of antioxidant activities of extracts

RSC Advances ◽  
2015 ◽  
Vol 5 (83) ◽  
pp. 67532-67540 ◽  
Author(s):  
Meng Luo ◽  
Jiao-Yang Hu ◽  
Zhuo-Yue Song ◽  
Jiao Jiao ◽  
Fan-Song Mu ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Hplc Method ◽  
Ultrasound Assisted Extraction ◽  
Simultaneous Extraction ◽  
Highly Efficient ◽  
Crataegus Pinnatifida ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In this study, a highly efficient BBD–RSM optimized ultrasound-assisted extraction combined with HPLC method has been established for the simultaneous extraction and determination of CA, VG, VR, ORT, RT, VIT and HYP from Crataegus pinnatifida leaves.

scholarly journals A Fast and Efficient Ultrasound-Assisted Extraction of Tocopherols in Cow Milk Followed by HPLC Determination

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4645
Author(s):  
Archimede Rotondo ◽  
Giovanna Loredana La Torre ◽  
Teresa Gervasi ◽  
Giacomo di Matteo ◽  
Mattia Spano ◽  
...  
Keyword(s):  
Cattle Breed ◽  
Hplc Method ◽  
Cow Milk ◽  
Ultrasound Assisted Extraction ◽  
Fluorescence Detector ◽  
Milk Samples ◽  
Assisted Extraction ◽  
Relative Standard ◽  
Ultrasound Assisted

A fast HPLC method with fluorescence detector (FD) was developed for the determination of three tocopherols (TOCs) in milk samples from Modicana cattle breed. The ultrasound-assisted procedure was optimized for the extraction of TOCs prior to HPLC/FD analysis, reducing sample preparation time and allowing a fast quantification of α-tocopherol, δ-tocopherol and γ tocopherol. The optimized ultrasonic extraction combines an efficient and simple saponification at room temperature and a rapid HPLC quantification of TOCs in milk. The precision of the full analytical procedure was satisfactory and the recoveries at three spiked levels were between 95.3% and 87.8%. The linear correlations were evaluated (R2 > 0.99) and the relative standard deviation (RSD) values for intra-day and inter-day tests at three spiked levels were below 1% for the retention time and below 5.20% for the area at low level spiking. The proposed procedure, reducing the experimental complexity, allowed accurate extraction and detection of three TOCs in milk samples from Modicana cattle breed.

Innovative Extraction Techniques for the Optimum Extraction of Phenolic Compounds from Peanut Meal and Evaluation of Their Biological Activities

2019 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Phenolic Compounds ◽  
Carcinoma Cell ◽  
Biological Activities ◽  
Peanut Meal ◽  
Ultrasound Assisted Extraction ◽  
Carcinoma Cell Lines ◽  
Assisted Extraction ◽  
Phenolic Extract ◽  
Ultrasound Assisted ◽  
Water Ratio

There is a worldwide demand for phenolic compounds (PC) because they exhibit several biological activities. This work aimed at extracting phenolic compounds from peanut meal. The methods of extraction were mainly: conventional solvent extraction (traditional methods) and ultrasound assisted extraction (recent methods) and comparing their results. Peanut meal (PM) was prepared by defatting with n-hexane, and then extracted by the two previous methods. First, the conventional solvents used were 80% methanol, ethanol, acetone, isopropanol, and distilled water. Then studied Different parameters such as meal: water ratio, also the effect of temperature and the pH on the extraction process. Second, ultrasonic assisted extractions (USAE), the parameters investigated were temperature, time and speed of sonication. Finally, all the extracts were analyzed by HPLC for their phenolic contents. Results indicated that the highest extracted PC achieved by solvents was in distilled water where 1:100, Meal: Water ratio which extracted 40 mg PC / g PM at 30& 35°C. Highest extracted PC was achieved by alkaline medium at pH 12 more than acidic and neutral medium. While (USAE) at speed 8 ultrasonication and temperature 30ᵒC, extracted 49.2mg PC /g PM. Sothe ultrasound assisted extraction exhibited great influence on the extraction of phenolic compounds from peanut meal. The ultrasonic peanut extract was examined for its antioxidant, antimicrobial and anticarcinogenic activities. The antioxidant activity of PM phenolic extract prepared by ultrasonic technique, was measured by, β-carotene, and DPPH methods, and reducing antioxidant power. Results revealed values: 84.57, 57.72 and 5960 respectively. The PM extract showed different levels of antimicrobial activity against the pathogenic bacteria used. As for the anticarcinogenic effect PM phenolic extract most effective on inhibiting colon carcinoma and lung carcinoma cell lines with IC50 = 20.7 and 20.8 µ/ml., respectively. This was followed by intestinal carcinoma and liver carcinoma cell lines with IC50= 39.6 and 40.2µ/ml.

Greener ultrasound-assisted extraction of bioactive phenolic compounds in Croton heliotropiifolius Kunth leaves

2020 ◽  
Vol 159 ◽  
pp. 105525 ◽  
Author(s):  
Cheila B. do C. de Sousa ◽  
Gilvanda L. dos Anjos ◽  
Rafaela S.A. Nóbrega ◽  
Andréia da S. Magaton ◽  
Fabrício M. de Miranda ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted
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