Dilute acid pretreatment of rice straw, structural characterization and optimization of enzymatic hydrolysis conditions by response surface methodology

RSC Advances ◽  
2015 ◽  
Vol 5 (58) ◽  
pp. 46525-46533 ◽  
Author(s):  
Siddheshwar Dnyandev Kshirsagar ◽  
Pankajkumar Ramdas Waghmare ◽  
Prakash Chandrakant Loni ◽  
Sushama Anandrao Patil ◽  
Sanjay Prabhu Govindwar
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Production Technology ◽  
Structural Characterization ◽  
Dilute Acid Pretreatment ◽  
Fermentable Sugars ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Conditions ◽  
A Current

Efficient conversion of fermentable sugars from cheap lignocellulosic biomass is a current need in viable ethanol production technology.

scholarly journals Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Wenqian Lin ◽  
Jinlai Yang ◽  
Yayue Zheng ◽  
Caoxing Huang ◽  
Qiang Yong
Keyword(s):  
Enzymatic Hydrolysis ◽  
Negative Impact ◽  
Dilute Acid Pretreatment ◽  
Residual Lignin ◽  
Dilute Acid ◽  
Enzymatic Digestibility ◽  
Acid Pretreatment ◽  
Organic Reagents ◽  
Milled Wood Lignin ◽  
Affinity Constants

Abstract Background During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. However, how these lignins interact with cellulase enzymes and then affect enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid-pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by the milled wood lignin (MWL) method. All of the lignin fractions obtained from DAP-BR were used to investigate the mechanism for interaction between lignin and cellulase using surface plasmon resonance (SPR) technology to understand how they affect enzymatic hydrolysis Results The results showed that removing surface lignin significantly decreased the yield for enzymatic hydrolysis DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel inhibited its enzymatic hydrolysis, while different SL samples showed slight increases in enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus SL samples, a stronger affinity for MWL (KD = 6.8–24.7 nM) was found versus that of SL (KD = 39.4–52.6 nM) by SPR analysis. The affinity constants of all tested lignins exhibited good correlations (r > 0.6) with the effects on enzymatic digestibility of extracted DAP-BR and Avicel. Conclusions This work revealed that the surface lignin on DAP-BR is necessary for maintaining enzyme digestibility levels, and its removal has a negative impact on substrate digestibility.

scholarly journals Investigation of interaction between cellulase and residual lignin fractions in acid-pretreated bamboo residues and their effect on enzymatic digestibility

2021 ◽  
Author(s):  
Wenqian Lin ◽  
Jinlai Yang ◽  
Yayue Zheng ◽  
Caoxing Huang ◽  
Qiang Yong
Keyword(s):  
Enzymatic Hydrolysis ◽  
Negative Impact ◽  
Interaction Mechanism ◽  
Dilute Acid Pretreatment ◽  
Affinity Constant ◽  
Residual Lignin ◽  
Dilute Acid ◽  
Enzymatic Digestibility ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Abstract Background: During dilute acid pretreatment, pseudo lignin and lignin form droplets which deposit on the surface of lignocellulose, and further inhibit its enzymatic hydrolysis. However, how this lignin interacts with cellulase enzymes and then affects enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by milled wood lignin (MWL) method. All the obtained lignin fractions from DAP-BR were used to investigate the interaction mechanism between lignin and cellulase using surface plasmon resonance (SPR) technology in order to understand how they affect enzymatic hydrolysisResults: Results showed that removing surface lignin significantly decrease the enzymatic hydrolysis of DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel decreased enzymatic hydrolysis of Avicel, while different SL samples showed a slight increase to its enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus the SL samples, stronger affinity for MWL (KD = 6.8-24.7 nM) was found versus that of SL (KD = 39.4-52.6 nM) by SPR analysis. The affinity constant of all tested lignin had good correlations (R2>0.6) with their effects on enzymatic digestibility of extracted DAP-BR and Avicel.Conclusions: This work reveals that the surface lignin on DAP-BR is necessary towards maintaining enzyme digestibility levels, and its removal has a negative impact on the substrate’s digestibility.

scholarly journals Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Vaibhav V. Goud ◽  
Venkata V. Dasu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Crystallinity Index ◽  
Sem Analysis ◽  
Reducing Sugar ◽  
Dilute Acid Pretreatment ◽  
Crystalline Cellulose ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

scholarly journals Optimization of Alkaline and Dilute Acid Pretreatment of Agave Bagasse by Response Surface Methodology

2015 ◽  
Vol 3 ◽  
Author(s):  
Abimael I. Ávila-Lara ◽  
Jesus N. Camberos-Flores ◽  
Jorge A. Mendoza-Pérez ◽  
Sarah R. Messina-Fernández ◽  
Claudia E. Saldaña-Duran ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment

Dilute Acid Pretreatment of Wheat Straw: A Predictive Model for Energy Consumption Using Response Surface Methodology

2013 ◽  
Author(s):  
Xiaoxu Song ◽  
Meng Zhang ◽  
Z. J. Pei ◽  
A. J. Nottingham ◽  
P. F. Zhang
Keyword(s):  
Response Surface Methodology ◽  
Energy Consumption ◽  
Response Surface ◽  
Minimum Energy ◽  
Solid Content ◽  
Dilute Acid Pretreatment ◽  
Process Conditions ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Minimum Energy Consumption

Response surface methodology was used to study the effects of parameters namely, time, temperature, and solid content and to optimize the process conditions for the minimum energy consumption in dilute acid pretreatment. Box-Behnken design using response surface methodology was employed. Effects of time and temperature are significant at the significant level of α = 0.05. Longer time and higher temperature result in higher power energy consumption. The best optimal values of the process conditions are time 14–21 min and temperature 129–139 °C.

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