Locked nucleic acid modified bi-specific aptamer-targeted nanoparticles carrying survivin antagonist towards effective colon cancer therapy

RSC Advances ◽  
2015 ◽  
Vol 5 (37) ◽  
pp. 29008-29016 ◽  
Author(s):  
Kislay Roy ◽  
Rupinder K. Kanwar ◽  
Chun Hei Antonio Cheung ◽  
Cassandra Lee Fleming ◽  
Rakesh N. Veedu ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Nucleic Acid ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Locked Nucleic Acid ◽  
Targeted Nanoparticles ◽  
Target Cancer

EpCAM and nucleolin translocate into the cytoplasm and nucleus that facilitates enhanced uptake of nanocarrier to specifically target cancer cells.

scholarly journals Knockdown of Parkinson’s disease-related gene ATP13A2 reduces tumorigenesis via blocking autophagic flux in colon cancer

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Qian Chen ◽  
Li Zhong ◽  
Chao Zhou ◽  
Yan Feng ◽  
Quan-xing Liu ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Colon Cancer ◽  
Parkinson's Disease ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Cancer Prognosis ◽  
Autophagic Flux ◽  
Colon Cancer Cells ◽  
Related Gene ◽  
Disease Related Gene

Abstract Background Accumulating evidence shows that Parkinson’s disease is negatively associated with colon cancer risk, indicating that Parkinson’s disease family proteins may be involved in the initiation of colon cancer. Here, we aimed to identify a Parkinson’s disease-related gene involved in colon cancer, elucidate the underlying mechanisms, and test whether it can be used as a target for cancer therapy. Methods We first screened colon cancer and normal tissues for differential expression of Parkinson’s disease-associated genes and identified ATP13A2, which encodes cation-transporting ATPase 13A2, as a putative marker for colon cancer. We next correlated ATP13A2 expression with colon cancer prognosis. We performed a series of ATP13A2 knockdown and overexpression studies in vitro to identify the contribution of ATP13A2 in the stemness and invasive capacity of colon cancer cells. Additionally, autophagy flux assay were determined to explore the mechanism of ATP13A2 induced stemness. Finally, we knocked down ATP13A2 in mice using siRNA to determine whether it can be used as target for colon cancer treatment. Results Colon cancer patients with high ATP13A2 expression exhibit shorter overall survival than those with low ATP13A2. Functionally, ATP13A2 acts as a novel stimulator of stem-like traits. Furthermore, knockdown of ATP13A2 in HCT116 resulted in decreased levels of cellular autophagy. Additionally, bafilomycin A1, an autophagy inhibitor, reversed the ATP13A2-induced stemness of colon cancer cells. Lastly treatment with ATP13A2 siRNA reduced the volume of colon cancer xenografts in mice. Conclusions The PD-associated gene ATP13A2 is involved in colon cancer stemness through regulation of autophagy. Furthermore, ATP13A2 is a novel prognostic biomarker for colon cancer and is a potential target for colon cancer therapy.

scholarly journals pH-operated hybrid silica nanoparticles with multiple H-bond stoppers for colon cancer therapy

RSC Advances ◽  
2015 ◽  
Vol 5 (80) ◽  
pp. 64932-64936 ◽  
Author(s):  
C. Théron ◽  
A. Gallud ◽  
S. Giret ◽  
M. Maynadier ◽  
D. Grégoire ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Therapy ◽  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Cyanuric Acid ◽  
Carcinoma Cells ◽  
Controlled Delivery ◽  
Chemotherapeutic Drug ◽  
Colon Carcinoma Cells ◽  
Hybrid Silica

Controlled delivery of a chemotherapeutic drug from pH-sensitive hybrid silica nanocarriers efficiently targets colon carcinoma cells. The drug, blocked by cyanuric acid as stopper, is autonomously released inside the cancer cells.

MDM-2 Overexpression in Human Stem-Like and Mature Colon Cancer Cells as a Target for Anti-Cancer Therapy: Steps toward a Translational Model

2015 ◽  
Vol 221 (4) ◽  
pp. S142
Author(s):  
Mohammad F. Shaikh ◽  
Blake D. Babcock ◽  
Elizabeth Gleeson ◽  
Patrice Love ◽  
Katlin Davitt ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Translational Model ◽  
Anti Cancer

scholarly journals MAZ-binding G4-decoy with locked nucleic acid and twisted intercalating nucleic acid modifications suppresses KRAS in pancreatic cancer cells and delays tumor growth in mice

2013 ◽  
Vol 41 (7) ◽  
pp. 4049-4064 ◽  
Author(s):  
Susanna Cogoi ◽  
Sonia Zorzet ◽  
Valentina Rapozzi ◽  
Imrich Géci ◽  
Erik B. Pedersen ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Nucleic Acid ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Locked Nucleic Acid ◽  
Pancreatic Cancer Cells

Delivering curcumin and gemcitabine in one nanoparticle platform for colon cancer therapy

RSC Advances ◽  
2014 ◽  
Vol 4 (106) ◽  
pp. 61948-61959 ◽  
Author(s):  
Manhong Tan ◽  
Jia Luo ◽  
Ying Tian
Keyword(s):  
Colon Cancer ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Colon Cancer Cells

As gemcitabine and curcumin have different targets in colon cancer cells, combination of them may bring benefits.

scholarly journals Pharmacological Inhibition of miR-130 Family Suppresses Bladder Tumor Growth by Targeting Various Oncogenic Pathways via PTPN1

2021 ◽  
Vol 22 (9) ◽  
pp. 4751
Author(s):  
Yuya Monoe ◽  
Kentaro Jingushi ◽  
Akitaka Kawase ◽  
Takayuki Hirono ◽  
Ryo Hirose ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Nucleic Acid ◽  
Cell Growth ◽  
Cancer Cells ◽  
Fiber Formation ◽  
Negative Regulator ◽  
Locked Nucleic Acid ◽  
Luciferase Assay ◽  
Pharmacological Inhibition ◽  
Bladder Cancer Cells

Previously, we have revealed that the miR-130 family (miR-130b, miR-301a, and miR-301b) functions as an oncomiR in bladder cancer. The pharmacological inhibition of the miR-130 family molecules by the seed-targeting strategy with an 8-mer tiny locked nucleic acid (LNA) inhibits the growth, migration, and invasion of bladder cancer cells by repressing stress fiber formation. Here, we searched for a functionally advanced target sequence with LNA for the miR-130 family with low cytotoxicity and found LNA #9 (A(L)^i^i^A(L)^T(L)^T(L)^G(L)^5(L)^A(L)^5(L)^T(L)^G) as a candidate LNA. LNA #9 inhibited cell growth in vitro and in an in vivo orthotopic bladder cancer model. Proteome-wide tyrosine phosphorylation analysis suggested that the miR-130 family upregulates a wide range of receptor tyrosine kinases (RTKs) signaling via the expression of phosphorylated Src (pSrcTyr416). SILAC-based proteome analysis and a luciferase assay identified protein tyrosine phosphatase non-receptor type 1 (PTPN1), which is implicated as a negative regulator of multiple signaling pathways downstream of RTKs as a target gene of the miR-130 family. The miR-130-targeted LNA increased and decreased PTPN1 and pSrcTyr416 expressions, respectively. PTPN1 knockdown led to increased tumor properties (cell growth, invasion, and migration) and increased pSrcTyr416 expression in bladder cancer cells, suggesting that the miR-130 family upregulates multiple RTK signaling by targeting PTPN1 and subsequent Src activation in bladder cancer. Thus, our newly designed miR-130 family targeting LNA could be a promising nucleic acid therapeutic agent for bladder cancer.

scholarly journals Biomimetic hollow mesoporous hydroxyapatite microsphere with controlled morphology, entrapment efficiency and degradability for cancer therapy

RSC Advances ◽  
2017 ◽  
Vol 7 (71) ◽  
pp. 44788-44798 ◽  
Author(s):  
Wei Qiao ◽  
Xinmiao Lan ◽  
James K. H. Tsoi ◽  
Zhuofan Chen ◽  
Richard Y. X. Su ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Sodium Dodecyl Sulfate ◽  
Cancer Cells ◽  
Sodium Dodecyl ◽  
Entrapment Efficiency ◽  
Carbonated Hydroxyapatite ◽  
Dodecyl Sulfate ◽  
Controlled Morphology ◽  
Target Cancer

Hollow mesoporous carbonated hydroxyapatite microspheres produced via sodium dodecyl sulfate assisted precipitation can target cancer cells through acidic dependent release of loaded CDDP.

A self-assembled peptide nucleic acid–microRNA nanocomplex for dual modulation of cancer-related microRNAs

2019 ◽  
Vol 55 (14) ◽  
pp. 2106-2109 ◽  
Author(s):  
Xingxing Wang ◽  
Xiao Xiao ◽  
Bingsen Zhang ◽  
Jinbo Li ◽  
Yan Zhang
Keyword(s):  
Nucleic Acid ◽  
Cancer Cells ◽  
Peptide Nucleic Acid ◽  
Self Assembled ◽  
Target Cancer

A peptide nucleic acid–microRNA nanocomplex was prepared and used for dual-modulation of cancer-related microRNAs in target cancer cells.

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