Neurotoxin-directed synthesis and in vitro evaluation of Au nanoclusters

RSC Advances ◽  
2015 ◽  
Vol 5 (38) ◽  
pp. 29647-29652 ◽  
Author(s):  
Zhengbo Sun ◽  
Wenlu Zhang ◽  
Pengfei Zhang ◽  
Duyang Gao ◽  
Ping Gong ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Glioma Cells ◽  
High Specificity ◽  
Au Nanoclusters ◽  
In Vitro Evaluation ◽  
Theranostic Agent ◽  
Bright Fluorescence ◽  
Directed Synthesis

A glioma-specific theranostic agent is prepared by using Chlorotoxin fusion protein to direct the synthesis of Au nanoclusters, which exhibit bright fluorescence and high specificity to target and treat glioma cells.

In Vitro Evaluation of Iodine-125-Labeled Monoclonal Antibody (MAb 425) in Human High-Grade Glioma Cells

1996 ◽  
Vol 19 (6) ◽  
pp. 601-608 ◽  
Author(s):  
Jacqueline G. Emrich ◽  
Hans Bender ◽  
Reiner Class ◽  
Jeffrey Eshleman ◽  
Curtis Miyamoto ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Glioma Cells ◽  
High Grade Glioma ◽  
High Grade ◽  
In Vitro Evaluation ◽  
Iodine 125

scholarly journals Targeted DNA transposition using a dCas9-transposase fusion protein

2019 ◽  
Author(s):  
Shivam Bhatt ◽  
Ronald Chalmers
Keyword(s):  
Fusion Protein ◽  
Binding Site ◽  
Biochemical Analysis ◽  
Genome Engineering ◽  
High Specificity ◽  
Target Dna ◽  
Nucleoprotein Complexes ◽  
Significant Conformational Change

SUMMARYHomology directed genome engineering is limited by transgene size. Although DNA transposons are more efficient with large transgenes, random integrations are potentially mutagenic. Catalytically inactive Cas9 is attractive candidate for targeting a transposase fusion-protein because of its high specificity and affinity for its binding site. Here we demonstrate efficient Cas9 targeting of a mariner transposon. Targeted integrations were tightly constrained at two adjacent TA dinucleotides about 20 bp to one side of the gRNA binding site. Biochemical analysis of the nucleoprotein complexes demonstrated that the transposase and Cas9 moieties of the fusion protein can bind their respective substrates independently. In the presence of the Cas9 target DNA, kinetic analysis revealed a delay between first and second strand cleavage at the transposon end. This step involves a significant conformational change that may be hindered by the properties of the interdomainal linker. Otherwise, the transposase behaved normally and was proficient for integration in vitro and in vivo.

In vitro evaluation of IL-18 effects in Crohn's disease

2001 ◽  
Vol 120 (5) ◽  
pp. A316-A317
Author(s):  
P MAERTEN ◽  
S COLPAERT ◽  
Z LIU ◽  
K GEBOES ◽  
J CEUPPENS ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
In Vitro Evaluation

52: In Vitro Evaluation of Ureteral Stent Compression

2006 ◽  
Vol 175 (4S) ◽  
pp. 18-18
Author(s):  
Kari Hendlin ◽  
Krishna Vedula ◽  
Christina Horn ◽  
Manoj Monga
Keyword(s):  
Ureteral Stent ◽  
In Vitro Evaluation
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