Photo-crosslinked hierarchically honeycomb-patterned/macroporous scaffolds of calcium phosphate cement promote MC3T3-E1 cell functions

RSC Advances ◽  
2015 ◽  
Vol 5 (45) ◽  
pp. 36007-36014 ◽  
Author(s):  
X. H. Wu ◽  
Z. Y. Wu ◽  
J. Qian ◽  
Y. G. Yan ◽  
J. Wei ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Cell Functions ◽  
Breath Figure ◽  
Breath Figure Method

Novel photo-crosslinked hierarchically honeycomb-patterned/macroporous scaffolds of calcium phosphate cement were fabricated through a facile process using a breath-figure method. MC3T3-E1 cell functions were significantly enhanced on these scaffolds compared to others.

Nano-hydroxyapatite promotes self-assembly of honeycomb pores in poly(l-lactide) films through breath-figure method and MC3T3-E1 cell functions

RSC Advances ◽  
2015 ◽  
Vol 5 (9) ◽  
pp. 6607-6616 ◽  
Author(s):  
X. H. Wu ◽  
Z. Y. Wu ◽  
J. C. Su ◽  
Y. G. Yan ◽  
B. Q. Yu ◽  
...  
Keyword(s):  
Self Assembly ◽  
Cell Functions ◽  
Breath Figure ◽  
Breath Figure Method

The effects of nano-hydroxyapatite particles on the formation of honeycomb poly(l-lactide) films and MC3T3-E1 cell functions were investigated.

scholarly journals A bone replacement-type calcium phosphate cement that becomes more porous in vivo by incorporating a degradable polymer

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Akiyoshi Shimatani ◽  
Hiromitsu Toyoda ◽  
Kumi Orita ◽  
Yuta Ibara ◽  
Yoshiyuki Yokogawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Alginic Acid ◽  
Setting Time ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Degradable Polymer ◽  
Low Viscosity ◽  
Bone Replacement

AbstractThis study investigated whether mixing low viscosity alginic acid with calcium phosphate cement (CPC) causes interconnected porosity in the CPC and enhances bone replacement by improving the biological interactions. Furthermore, we hypothesized that low viscosity alginic acid would shorten the setting time of CPC and improve its strength. CPC samples were prepared with 0, 5, 10, and 20% low viscosity alginic acid. After immersion in acetate buffer, possible porosification in CPC was monitored in vitro using scanning electron microscopy (SEM), and the setting times and compressive strengths were measured. In vivo study was conducted by placing CPC in a hole created on the femur of New Zealand white rabbit. Microcomputed tomography and histological examination were performed 6 weeks after implantation. SEM images confirmed that alginic acid enhanced the porosity of CPC compared to the control, and the setting time and compressive strength also improved. When incorporating a maximum amount of alginic acid, the new bone mass was significantly higher than the control group (P = 0.0153). These biological responses are promising for the translation of these biomaterials and their commercialization for clinic applications.

scholarly journals Enhanced Osteogenesis of Dental Pulp Stem Cells In Vitro Induced by Chitosan–PEG-Incorporated Calcium Phosphate Cement

Polymers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 2252
Author(s):  
Jae Eun Kim ◽  
Sangbae Park ◽  
Woong-Sup Lee ◽  
Jinsub Han ◽  
Jae Woon Lim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Zeta Potential ◽  
Mechanical Strength ◽  
Calcium Phosphate Cement ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Ion Trapping ◽  
Alizarin Red

The use of bone graft materials is required for the treatment of bone defects damaged beyond the critical defect; therefore, injectable calcium phosphate cement (CPC) is actively used after surgery. The application of various polymers to improve injectability, mechanical strength, and biological function of injection-type CPC is encouraged. We previously developed a chitosan–PEG conjugate (CS/PEG) by a sulfur (VI) fluoride exchange reaction, and the resulting chitosan derivative showed high solubility at a neutral pH. We have demonstrated the CPC incorporated with a poly (ethylene glycol) (PEG)-grafted chitosan (CS/PEG) and developed CS/PEG CPC. The characterization of CS/PEG CPC was conducted using Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The initial properties of CS/PEG CPCs, such as the pH, porosity, mechanical strength, zeta potential, and in vitro biocompatibility using the WST-1 assay, were also investigated. Moreover, osteocompatibility of CS/PEG CPCs was carried out via Alizarin Red S staining, immunocytochemistry, and Western blot analysis. CS/PEG CPC has enhanced mechanical strength compared to CPC, and the cohesion test also demonstrated in vivo stability. Furthermore, we determined whether CS/PEG CPC is a suitable candidate for promoting the osteogenic ability of Dental Pulp Stem Cells (DPSC). The elution of CS/PEG CPC entraps more calcium ion than CPC, as confirmed through the zeta potential test. Accordingly, the ion trapping effect of CS/PEG is considered to have played a role in promoting osteogenic differentiation of DPSCs. The results strongly suggested that CS/PEG could be used as suitable additives for improving osteogenic induction of bone substitute materials.

Behaviour of an injectable calcium phosphate cement with added tetracycline

2004 ◽  
Vol 274 (1-2) ◽  
pp. 261-268 ◽  
Author(s):  
A Ratier ◽  
M Freche ◽  
J.L Lacout ◽  
F Rodriguez
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement
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