Development of a monoclonal antibody-based ELISA for the detection of the novel insecticide cyantraniliprole

RSC Advances ◽  
2015 ◽  
Vol 5 (45) ◽  
pp. 35874-35881 ◽  
Author(s):  
Rui Zhang ◽  
Kechang Liu ◽  
Yongliang Cui ◽  
Wei Zhang ◽  
Lishan He ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Hplc Method ◽  
The Novel ◽  
Highly Sensitive

A highly sensitive and selective immunoassay was developed for the analysis of cyantraniliprole. The concentrations of cyantraniliprole residues in pakchoi samples determined by ELISA agreed with those by the HPLC method.

A Highly Sensitive Radioimmunoassay for Human Growth Hormone Using a Monoclonal Antibody

1984 ◽  
Vol 5 (3-4) ◽  
pp. 145-157 ◽  
Author(s):  
Françoise Gomez ◽  
Guy Pirens ◽  
Charles Schaus ◽  
Jean Closset ◽  
Georges Hennen
Keyword(s):  
Monoclonal Antibody ◽  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Highly Sensitive

scholarly journals Highly sensitive biomarker biosensor for early detection of cancer biomarkers

2021 ◽  
Author(s):  
Jaydev Upponi ◽  
Tiziana Musacchio ◽  
Salome Siavoshi ◽  
Asanterabi Malima ◽  
Cihan Yilmaz ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Early Detection ◽  
Carcinoembryonic Antigen ◽  
Early Detection Of Cancer ◽  
Cancer Biomarkers ◽  
Diagnostic Tools ◽  
Detection Systems ◽  
Highly Sensitive ◽  
Current Detection ◽  
Insufficient Sensitivity

Abstract Biomarkers are emerging as potentially important diagnostic tools for cancer and many other diseases. However, many current detection systems for suffer from insufficient sensitivity. To address this concern, we developed a highly sensitive biosensor, featuring monoclonal antibody-coated polystyrene nanobeads assembled in the trenches of a microchip, for the detection of cancer biomarkers. These biosensors detected nucleosomes and carcinoembryonic antigen in serum at concentrations of 62.5 and 15.6 pg/mL, respectively. Very low detection limits that suggest such devices might be beneficial for the early detection of tumors and for monitoring of patients in remission.

Concurrent Determination of Daclatasvir and Sofosbuvir in Pure Binary Mixture and Their Combined Film Coated Tablets by a Simple Stability Indicating RP-HPLC Method

2021 ◽  
pp. 5913-5918
Author(s):  
Ramreddy Godela ◽  
Sowjanya G
Keyword(s):  
Degradation Products ◽  
Hplc Method ◽  
Forced Degradation ◽  
Good Resolution ◽  
Stability Indicating ◽  
Extreme Stress ◽  
Rp Hplc ◽  
Highly Sensitive ◽  
Validation Parameters ◽  
Acid And Base

A trouble-free, simple, specific and highly sensitive stability indicating phase HPLC method was developed for concurrent assessment of Daclatasvir and Sofosbuvir in pure and in their combined tablet formulation. An effectual separation was accomplished by using XDB Phenyl (250 x 4.6mm, 5µ,100 A0) column, mobile phase composition of Acetonitrile: buffer(0.1%v/v Trifluoroaceticacid in water) (50:50 v/v) and isocratic elution at a flow rate of 1ml/min and detection wavelength of 275nm. The extreme stress conditions like hydrolysis with acid and base, peroxide oxidation, thermal decomposition were used as per ICH specifications to assess the stability of the analytes in bulk and dosage forms. The retention times of Daclatasvir and Sofosbuvir were found at 2.8 and 3.7min respectively. The proposed method has linear response in the concentration ranges from 12 to 36µg/ml and 80 to 240 µg/ml for Daclatasvir and Sofosbuvir respectively. The detection and quantification limits calculated as 2.5μg/ml and 7.8μg/ml for DCL, 5.2μg/ml and 15.8μg/ml SOF respectively. All the method validation parameters were met the acceptance limits of Q2 specifications of ICH procedures. The degradation products produced by forced degradation studies were have good resolution from Daclatasir and Sofosbuvir peaks, which represents the methods stability. The proposed RP-HPLC method was highly sensitive, precise, stability indicating and economical. That’s why the method has the capacity to employ in the pharmaceutical manufacturing of Daclatasvir and Sofosbuvir and routine analysis in quality control department.

scholarly journals Highly Sensitive and Selective Fluorescent Detection of Gossypol Based on BSA-Stabilized Copper Nanoclusters

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 95 ◽  
Author(s):  
Shuangjiao Xu ◽  
Kehai Zhou ◽  
Dan Fang ◽  
Lei Ma
Keyword(s):  
High Performance ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Hplc Method ◽  
Fluorescent Detection ◽  
Copper Nanoclusters ◽  
Highly Sensitive ◽  
The Us ◽  
Fluorescence Quenching Mechanism ◽  
First Time

In this paper, fluorescent copper nanoclusters (NCs) are used as a novel probe for the sensitive detection of gossypol for the first time. Based on a fluorescence quenching mechanism induced by interactions between bovine serum albumin (BSA) and gossypol, fluorescent BSA-Cu NCs were seen to exhibit a high sensitivity to gossypol in the range of 0.1–100 µM. The detection limit for gossypol is 25 nM at a signal-to-noise ratio of three, which is approximately 35 times lower than the acceptable limit (0.9 µM) defined by the US Food and Drug Administration for cottonseed products. Moreover, the proposed method for gossypol displays excellent selectivity over many common interfering species. We also demonstrate the application of the present method to the measurement of several real samples with satisfactory recoveries, and the results agree well with those obtained using the high-performance liquid chromatography (HPLC) method. The method based on Cu NCs offers the followings advantages: simplicity of design, facile preparation of nanomaterials, and low experimental cost.

Two-site enzyme immunoassay of CD4 and CD8 molecules on the surface of T lymphocytes from healthy subjects and HIV-1-infected patients

1994 ◽  
Vol 40 (1) ◽  
pp. 30-37 ◽  
Author(s):  
D Carriere ◽  
C Fontaine ◽  
A M Berthier ◽  
A M Rouquette ◽  
P Carayon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
T Lymphocytes ◽  
Enzyme Immunoassay ◽  
Cd8 T Cells ◽  
Envelope Glycoprotein ◽  
Healthy Subjects ◽  
Gp 120 ◽  
Highly Sensitive ◽  
One Step ◽  
Hiv 1

Abstract A highly sensitive two-site enzyme immunoassay (Capcellia) was developed to determine the concentration of CD4 and CD8 molecules expressed on the surface of human T lymphocytes. This assay, performed in one step (20 min), involves the specific immunocapture of T lymphocytes and reaction of the CD4 or CD8 molecules with an enzyme-labeled monoclonal antibody (mAb). The results were expressed as molar concentrations of the T-cell markers on the basis of results obtained with calibrated CD4 and CD8 standards. The assay was sensitive enough to detect 0.4 pmol/L CD4 or 0.8 pmol/L CD8, which corresponded to approximately 20 x 10(6) CD4+ or CD8+ T cells per liter of blood. Mean concentrations in healthy adults were 17.2 pmol/L for CD4 and 22.1 pmol/L for CD8. The CD4 concentration was < 8 pmol/L in 50% of HIV-1-infected patients and in 95% of AIDS patients. Given the epitopic specificity of the mAb to CD4 we used, these values correspond to the concentration of CD4 molecules free of envelope glycoprotein (gp)120.

scholarly journals HPLC Estimation of New Impurity Methyl Ezetimibe in Ezetimibe Drug

2020 ◽  
Vol 32 (6) ◽  
pp. 1309-1313
Author(s):  
Duggirala Parvatha Venkata Vardhani Devi ◽  
Kapavarapu Maruthi Venkata Narayanarao ◽  
Pulipaka Shyamala ◽  
Rallabhandi Murali Krishna ◽  
Komali Siva Prasad
Keyword(s):  
Limit Of Detection ◽  
Signal To Noise Ratio ◽  
Gradient Elution ◽  
Hplc Method ◽  
The Novel ◽  
Signal To Noise ◽  
Drug Substances ◽  
Chromatographic Detection ◽  
Noise Ratio ◽  
Gradient Elution Mode

A new gradient elution mode HPLC method was developed and validated to detect and monitor the novel impurity namely methyl ezitimibe in ezetimibe drug substances. Chromatographic detection and analysis of methyl ezetimibe was performed on XBridge C18 column with mobile phase consisting of 0.02 M phosphate buffer (pH 5) and acetonitrile with 1 mL/min flow rate in gradient elution mode. Methyl ezetimibe was detected and monitored at 248 nm. The calibration curve was linear over range of 0.015 to 0.219% concentration. The limit of detection and quantification were computed as 0.005% (signal to noise ratio 3.60) and 0.015% (signal to noise ratio 15.96), respectively. The precision was 0.97% (%RSD) and accuracy was 93.2 to 98.2% (recovery). The developed method was proved suitable to detect and monitor methyl ezetimibe impurity in ezetimibe drug substances.

scholarly journals Dual Wavelength Imaging Allows Analysis of Membrane Fusion of Influenza Virus inside Cells

2006 ◽  
Vol 80 (4) ◽  
pp. 2013-2018 ◽  
Author(s):  
Tatsuya Sakai ◽  
Masanobu Ohuchi ◽  
Masaki Imai ◽  
Takafumi Mizuno ◽  
Kazunori Kawasaki ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Membrane Fusion ◽  
Imaging Technique ◽  
Rapid Analysis ◽  
Virus Infectivity ◽  
Viral Fusion ◽  
The Novel ◽  
Influenza Virus Hemagglutinin ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

ABSTRACT Influenza virus hemagglutinin (HA) is a determinant of virus infectivity. Therefore, it is important to determine whether HA of a new influenza virus, which can potentially cause pandemics, is functional against human cells. The novel imaging technique reported here allows rapid analysis of HA function by visualizing viral fusion inside cells. This imaging was designed to detect fusion changing the spectrum of the fluorescence-labeled virus. Using this imaging, we detected the fusion between a virus and a very small endosome that could not be detected previously, indicating that the imaging allows highly sensitive detection of viral fusion.

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