Fluorescence sensing of glucose using glucose oxidase incorporated into a fluorophore-containing PNIPAM hydrogel

2016 ◽  
Vol 7 (10) ◽  
pp. 1907-1912 ◽  
Author(s):  
Yongkyun Kim ◽  
Geunseok Jang ◽  
Daigeun Kim ◽  
Jongho Kim ◽  
Taek Seung Lee
Keyword(s):  
Composite Material ◽  
Glucose Oxidase ◽  
Fluorescent Dyes ◽  
Ph Sensitive ◽  
Fluorescence Sensing

We present a new composite material composed of pH sensitive fluorescent dyes in a poly(N-isopropylacrylamide)-based hydrogel and incorporating glucose oxidase (GOx), which provides a platform for fluorescence sensing of glucose.

Pyridyl-indolizine derivatives as DNA binders and pH-sensitive fluorescent dyes

Tetrahedron ◽  
2016 ◽  
Vol 72 (50) ◽  
pp. 8215-8222 ◽  
Author(s):  
Narcisa-Laura Marangoci ◽  
Lacramioara Popovici ◽  
Elena-Laura Ursu ◽  
Ramona Danac ◽  
Lilia Clima ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Ph Sensitive ◽  
Dna Binders

Alginate beads containing pH-sensitive liposomes and glucose oxidase: glucose-sensitive release

2009 ◽  
Vol 287 (10) ◽  
pp. 1207-1214 ◽  
Author(s):  
Yeon Ji Hong ◽  
Hyeon Yong Lee ◽  
Jin-Chul Kim
Keyword(s):  
Glucose Oxidase ◽  
Alginate Beads ◽  
Ph Sensitive

scholarly journals Europium-Doped Ceria Nanocrystals as Nanozyme Fluorescent Probes for Biosensing

2021 ◽  
Vol 5 (1) ◽  
pp. 53
Author(s):  
Ali Othman ◽  
Akhtar Hayat ◽  
Silvana Andreescu
Keyword(s):  
Fluorescent Dyes ◽  
Aqueous Media ◽  
Doped Ceria ◽  
New Wave ◽  
Lactate Oxidase ◽  
Fluorescence Sensing ◽  
Combined Use ◽  
Direct Fluorescence ◽  
Average Size

Molecular nanoprobes with intrinsic enzyme-like activity represent a new wave of technology for rapid and sensitive detection of molecular targets. This work reports synthesis and characterization of novel and well-dispersed europium-doped ceria nanocrystals (EuCe NCs) with self-integrated catalytic and fluorescence sensing functions. The NCs have an average size of ∼5 nm and exhibit bright and stable fluorescence for more than 6 months in aqueous media. Their dual cooperative function as both a catalyst and fluorescent probe was explored to develop a universally applicable fluorescence-based biosensing method to monitor enzyme reactions and quantitatively measure clinically relevant molecules. Sensing capabilities are demonstrated for detection of H2O2, glucose/glucose oxidase, lactate/lactate oxidase, phosphatase activity, and the catecholamine neurotransmitter, dopamine. Results indicate that EuCe NCs not only provide high enzyme-mimetic activity, but also impart direct fluorescence sensing ability enabling all-in-one recognition, catalytic amplification, and the detection of biomolecular targets. The EuCe nanozyme offers a stable alternative to the more complex systems based on the combined use of natural enzymes and fluorescent dyes. The high stability and fluorescence detection capabilities demonstrate that EuCe NCs have the potential to be used as a generic platform in chemical and biological sensing and bioimaging applications.

scholarly journals Design of functionalized β-ketoenole derivatives as efficient fluorescent dyes for detection of amyloid fibrils

2018 ◽  
Vol 42 (16) ◽  
pp. 13308-13318 ◽  
Author(s):  
Vladyslava Kovalska ◽  
Svitlana Chernii ◽  
Mykhaylo Losytskyy ◽  
Iryna Tretyakova ◽  
Yan Dovbii ◽  
...  
Keyword(s):  
Amyloid Fibrils ◽  
Fluorescent Dyes ◽  
Fluorescence Sensing ◽  
Strong Emission

Functionalized β-ketoenoles for efficient fluorescence sensing of protein amyloid fibrils giving strong emission increase up to 0.5 QY are designed.

scholarly journals Studies on Conducting Composite MaterialGlucose Oxidase Biosensor

2003 ◽  
Vol 19 (06) ◽  
pp. 533-537
Author(s):  
Zhang Guo-Lin ◽  
◽  
Pan Xian-Hua ◽  
Kan Jin-Qing ◽  
Zhang Jing-Hui ◽  
...  
Keyword(s):  
Composite Material ◽  
Glucose Oxidase ◽  
Conducting Composite

scholarly journals Surface-modified Colloid CdTe/CdS Quantum Dots by a Biocompatible Thiazolidine Derivative as Promising Platform for Immobilization of Glucose Oxidase: Application to Fluorescence Sensing of Glucose

2021 ◽  
Author(s):  
Rahman Hallaj ◽  
Zahra Hosseinchi
Keyword(s):  
Quantum Dots ◽  
Glucose Oxidase ◽  
Limit Of Detection ◽  
Low Cost ◽  
Fluorescence Emission ◽  
Cds Quantum Dots ◽  
Fluorescence Sensing ◽  
Experimental Conditions ◽  
Glucose Determination ◽  
Cds Qds

Abstract This work focuses on the synthesis of novel modified core-shell CdTe/CdS quantum dots (QDs) and develops as a fluorescence sensor for glucose determination. The (E)-2,2'-(4,4'-dioxo-2,2'-dithioxo-2H,2'H-[5,5'-bithiazolylidene]-3,3'(4H,4'H)-diyl)bis(3- mercaptopropanoic acid) (DTM) as a new derivative of thiazolidine was synthesized and characterized and used to surface-modification of CdTe/CdS QDs. DTM-capped CdTe/CdS QDs used to immobilization of glucose oxidase (GOD). The intensity fluorescence emission of the CdSe/CdS-DTM/GOD is highly sensitive to the concentration of H2O2 as a byproduct of the catalytic oxidation of glucose. The experimental results showed that the quenched fluorescence was proportional to the glucose concentration within the range of 10 nM − 0.32 µM under optimized experimental conditions. The limit of detection of this system was found to be 4.3 nM. Compared with most of the existing methods, this newly developed system possesses many advantages, including simplicity, low cost, and good sensitivity.

Combined Fenton and starvation therapies using hemoglobin and glucose oxidase

Nanoscale ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 5705-5716 ◽  
Author(s):  
Hadi Ranji-Burachaloo ◽  
Amin Reyhani ◽  
Paul A. Gurr ◽  
Dave E. Dunstan ◽  
Greg G. Qiao
Keyword(s):  
Cancer Treatment ◽  
Glucose Oxidase ◽  
Ph Sensitive

Selective cancer treatment using combined Fenton and starvation therapies through hemoglobin and glucose oxidase encapsulated in pH-sensitive ZIF-8.

A New One-Pot Way to Prepare Enzyme-Containing Coordination Polymers by Sonochemistry

2021 ◽  
Vol 21 (5) ◽  
pp. 2935-2938
Author(s):  
Alessandra Scano ◽  
Valentina Cabras ◽  
Guido Ennas
Keyword(s):  
Composite Material ◽  
Glucose Oxidase ◽  
Coordination Polymers ◽  
Thermal Characterization ◽  
Zinc Salt ◽  
Sonochemical Method ◽  
One Pot ◽  
Conventional Methods ◽  
Short Time

Enzyme-containing Coordination Polymers (CPs) were prepared by a one-pot sonochemical method, mixing Glucose Oxidase (GOx) enzyme, 4,4′-bipyridine as spacer ligand, and chloride zinc salt. The reaction took place in a very short time (a few minutes) with a minimum use of solvents compared to conventional methods. The obtained composite material, named GOx-[Zn(4,4′-bipy)Cl2]∞, resulted from embedding the GOx molecules into uniformly sized [Zn(4,4′-bipy)Cl2] nanocrystals. The structural and thermal characterization, confirmed that the adopted strategy allows the preparation of hybrid bio-nanomaterials in a very fast, facile and eco-friendly method, by simply synthesising the [Zn(4,4′-bipy)Cl2] nanocrystals in the presence of the enzyme.

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