Surface modification of polydopamine coated particles via glycopolymer brush synthesis for protein binding and FLIM testing

2015 ◽  
Vol 6 (13) ◽  
pp. 2504-2511 ◽  
Author(s):  
Solomon Pradhan Le-Masurier ◽  
Hien Thi Thu Duong ◽  
Cyrille Boyer ◽  
Anthony Michael Granville
Keyword(s):  
Surface Modification ◽  
Protein Binding ◽  
Fluorescent Protein ◽  
Polymer Coatings ◽  
Coated Particles ◽  
Lifetime Analysis

Polymer coatings on silica cores as well as fluorescent protein binding and fluorescent lifetime analysis.

scholarly journals SURFACE MODIFICATION OF TITANIUM DIOXIDE NANOPARTICLES WITH ACRYLIC ACID/ISOBUTYLENE COPOLYMER UNDER ULTRASONIC TREATMENT

2019 ◽  
Vol 16 (32) ◽  
pp. 338-344
Author(s):  
Nikolay A. BULYCHEV ◽  
Lev N. RABINSKIY
Keyword(s):  
Titanium Dioxide ◽  
Surface Modification ◽  
Composite Materials ◽  
Acrylic Acid ◽  
Ultrasonic Treatment ◽  
Titanium Dioxide Nanoparticles ◽  
Polymer Coatings ◽  
Pigment Surface ◽  
Electrokinetic Sonic Amplitude ◽  
Modification Of Titanium

The influence of the ultrasonic treatment of block copolymer solution on its solid-liquid interface behavior was investigated in detail. The surface modification of titanium dioxide nanoparticles in aqueous dispersions of specially tailor-made periodic acrylic acid/isobutylene copolymer by ultrasonic treatment was studied in order to get new approaches for the creation of hybrid composite materials or polymer coatings. The pigment surface modification by the above copolymer was comparatively investigated regarding conventional adsorption as contrasted to an ultrasonic treatment assisted procedure. The course and efficiency of the polymer adsorption onto the pigment surface were quantified by electrokinetic sonic amplitude measurements. The higher efficiency of the pigment surface coating by the copolymer as achieved by ultrasonic treatment in comparison to conventional adsorption is a consequence of ultrasonically induced pigment surface activation. Two perspective avenues of the utilization of the discovered effects for creation of organic-inorganic composite materials are anticipated: the nanoparticles could first be treated by ultrasound in the presence of polymers and so create a surface modifying coating and the second option is an entrainment of the nanoparticles into the monomer matrix which can be polymerized afterward yielding a polymer with immobilized nanoparticles.

scholarly journals Controlled Surface Wettability by Plasma Polymer Surface Modification

Surfaces ◽  
2019 ◽  
Vol 2 (2) ◽  
pp. 349-371 ◽  
Author(s):  
Muzammil Iqbal ◽  
Duy Khoe Dinh ◽  
Qasim Abbas ◽  
Muhammad Imran ◽  
Harse Sattar ◽  
...  
Keyword(s):  
Surface Modification ◽  
Surface Chemistry ◽  
Plasma Polymerization ◽  
Polymer Surface ◽  
Real Life ◽  
Polymer Coatings ◽  
Surface Wettability ◽  
Smart Polymer ◽  
Wetting Behavior ◽  
Polymer Surface Modification

Inspired by nature, tunable wettability has attracted a lot of attention in both academia and industry. Various methods of polymer surface tailoring have been studied to control the changes in wetting behavior. Polymers with a precisely controlled wetting behavior in a specific environment are blessed with a wealth of opportunities and potential applications exploitable in biomaterial engineering. Controlled wetting behavior can be obtained by combining surface chemistry and morphology. Plasma assisted polymer surface modification technique has played a significant part to control surface chemistry and morphology, thus improving the surface wetting properties of polymers in many applications. This review focuses on plasma polymerization and investigations regarding surface chemistry, surface wettability and coating kinetics, as well as coating stability. We begin with a brief overview of plasma polymerization; this includes growth mechanisms of plasma polymerization and influence of plasma parameters. Next, surface wettability and theoretical background structures and chemistry of superhydrophobic and superhydrophilic surfaces are discussed. In this review, a summary is made of recent work on tunable wettability by tailoring surface chemistry with physical appearance (i.e. substrate texture). The formation of smart polymer coatings, which adjust their surface wettability according to outside environment, including, pH, light, electric field and temperature, is also discussed. Finally, the applications of tunable wettability and pH responsiveness of polymer coatings in real life are addressed. This review should be of interest to plasma surface science communality particularly focused controlled wettability of smart polymer surfaces.

Generation of mWasabi fluorescent protein-binding nanobodies

2020 ◽  
Vol 608 ◽  
pp. 113875
Author(s):  
Shufeng Li ◽  
Haitao Shan ◽  
Ting Wang ◽  
Xiaojuan Zheng ◽  
Minke Shi ◽  
...  
Keyword(s):  
Protein Binding ◽  
Fluorescent Protein

Surface Modification of Confined Microgeometries via Vapor-Deposited Polymer Coatings

2006 ◽  
Vol 128 (1) ◽  
pp. 374-380 ◽  
Author(s):  
Hsien-Yeh Chen ◽  
Yaseen Elkasabi ◽  
Joerg Lahann
Keyword(s):  
Surface Modification ◽  
Polymer Coatings

Surface modification of polymeric membranes for low protein binding

2010 ◽  
Vol 1 (2) ◽  
pp. 103-120 ◽  
Author(s):  
Akon Higuchi ◽  
Miho Tamai ◽  
Yoh-Ichi Tagawa ◽  
Yung Chang ◽  
Qing-Dong Ling
Keyword(s):  
Surface Modification ◽  
Protein Binding ◽  
Polymeric Membranes ◽  
Low Protein

scholarly journals Changing Times: Fluorescence-lifetime Analysis of Amyloidogenic SF-IAPP Fusion Protein

2018 ◽  
Author(s):  
Olga I Antimonova ◽  
Dmitry V Lebedev ◽  
Yana A Zabrodskaya ◽  
Natalia A Grudinina ◽  
Michael M Shavlovsky ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Fluorescence Lifetime ◽  
Fluorescent Protein ◽  
Islet Amyloid ◽  
Cellular Models ◽  
Fluorescent Lifetime Imaging Microscopy ◽  
Lifetime Analysis ◽  
Changing Times ◽  
Green Fluorescent ◽  
Average Fluorescence

AbstractThe fluorescence lifetime of the superfolder green fluorescent protein (SF) and the SF protein fused with islet amyloid polypeptide (SF-IAPP) were studied in polyacrylamide gel. It was shown that the SF average fluorescence lifetime under these conditions slightly differs from that of the SF-IAPP monomer. SF-IAPP does not lose the ability to form amyloid-like fibrils; meanwhile, the average fluorescence lifetime of the fusion protein in fibrils is reduced. We propose the application of Fluorescent-lifetime Imaging Microscopy (FLIM) to the measurement of average fluorescence lifetimes of fusion proteins (amyloidogenic protein–SF) in the context of studies using cellular models of conformational diseases.

scholarly journals Nanoscale Effects in Temperature Induced Polymer Coatings

2009 ◽  
Vol 3 (3) ◽  
pp. 209-212
Author(s):  
Nikolay Bulychev ◽  
◽  
Frederik Wurst ◽  
Viktor Fomin ◽  
Thadeus Schauer ◽  
...  
Keyword(s):  
Surface Modification ◽  
Surface Morphology ◽  
Lower Critical Solution Temperature ◽  
Polymer Coating ◽  
Polymer Solutions ◽  
Substrate Surface ◽  
Polymer Coatings ◽  
Solution Temperature ◽  
Critical Solution Temperature ◽  
Polymer Deposition

In this paper the results of recent studies on the application of lower critical solution temperature (LCST) phenomena of polymer solutions to the surface modification of flat and spherical substrates are reported. It has been found that controlled polymer deposition can be achieved at temperatures exceeding LCST. The obtained polymer coating exhibits a peculiar surface morphology and, if particles are introduced, can be highly effective in pigment dispersions stabilizing. It has been established that the temperature induced polymer deposition can be carried out as the finely dispersed component precipitation on the substrate surface that goes along with the polymer deposition at temperatures exceeding LCST, which in its turn allows to modify the pigments surface when finely dispersed additives are incorporated into the surface modifying coating.

Minimal promoter for the NAD+-specific glutamate dehydrogenase gene of Neurospora crassa

2002 ◽  
Vol 80 (2) ◽  
pp. 177-188 ◽  
Author(s):  
M Kapoor ◽  
C A Curle ◽  
S Kalia ◽  
Y Achari
Keyword(s):  
Protein Binding ◽  
Neurospora Crassa ◽  
Glutamate Dehydrogenase ◽  
Binding Sites ◽  
Fluorescent Protein ◽  
Cellular Protein ◽  
Upstream Sequence ◽  
Mobility Shift ◽  
Relative Level ◽  
Protein Binding Sites

The expression of the NAD+-specific glutamate dehydrogenase (NAD-GDH) gene of Neurospora crassa is subject to catabolite repression. To identify the minimal sequence necessary for promoter function, the 5'-flanking region of the NAD-GDH gene was screened for potential protein-binding sites. Fragments of DNA, containing sequences upstream from the ATG initiation codon, were employed as probes of Southwestern blots of total cellular protein from cells grown in media promoting repression and induction of NAD-GDH. Two polypeptides interacted differentially with a promoter probe; one was present in greater abundance in repressed cells and a higher relative level of the second was witnessed in induced cells. Electrophoretic mobility shift assays with labeled promoter fragments exhibited preferential interaction with proteins in the induced cultures. The upstream sequence containing the putative protein-binding sites was fused with the coding sequence of the green fluorescent protein (GFP). The resulting plasmid was introduced into the microconidia of an albino mutant of N. crassa by electroporation. Stable integration of the plasmid and expression of GFP in the hyphae and conidia of the transformants were demonstrated by Southern and Western blot analysis and fluorescence microscopy.Key words: Neurospora crassa, repression, induction, GFP fusion, electroporation, microconidia.

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