A two-photon off-on fluorescence probe for imaging thiols in live cells and tissues

2016 ◽  
Vol 15 (3) ◽  
pp. 412-419 ◽  
Author(s):  
Xinyue Zhu ◽  
Yan Li ◽  
Wenyan Zan ◽  
Jianjian Zhang ◽  
Zhenjie Chen ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Probe ◽  
Live Cells ◽  
Imaging Probe ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

An effective off-on two-photon fluorescence imaging probe for thiols was developed and successfully applied to image thiols both in live cells and tissues.

Open-Chain Crown-Ether-Derived Two-Photon Fluorescence Probe for Real-Time Dynamic Biopsy of Mercury Ions

2017 ◽  
Vol 70 (6) ◽  
pp. 705
Author(s):  
Chibao Huang ◽  
Daohai Zhang ◽  
Junle Qu ◽  
Xiaonan Liu ◽  
Guanglian Zhao ◽  
...  
Keyword(s):  
Water Solubility ◽  
Fluorescence Probe ◽  
Stokes Shift ◽  
Cell Permeability ◽  
Photon Absorption ◽  
Live Cells ◽  
Open Chain ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A novel two-photon fluorescence probe for Hg2+ derived from bis(styryl)terephthalonitrile, as a two-photon fluorophore, and bis[2-(2-hydroxyethyl sulfanyl) ethyl]amino group (ionophore), as a novel Hg2+ ligand, was developed. The probe possesses small molecule size, large two-photon absorption cross-section (1067 GM) in H2O, non-cytotoxic effect, long wavelength emission at 588 nm, large Stokes shift (121 nm), excellent photostability, high water solubility, good cell permeability, and pH insensitivity in the biologically relevant range. The probe can selectively detect Hg2+ ions in live cells and living tissues without interference from other metal ions and the membrane-bound probes, and its quenching constant is 8.73 × 105 M–1.

Two-photon fluorescence imaging of mitochondria viscosity with water-soluble pyridinium inner salts

2022 ◽  
Author(s):  
Bin Fang ◽  
Beilin Zhang ◽  
Rongxiu Zhai ◽  
Limin Wang ◽  
Yang Ding ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Rational Design ◽  
Fluorescence Probe ◽  
Water Soluble ◽  
Biological Applications ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Rational design of mitochondrial viscosity probes with water-soluble two-photon (TP) fluorescence probe still remains a challenge in practical biological applications. Herein, three novel water-soluble TP active sulfosalt derivatives (Mito-Z, Mito-H,...

scholarly journals Two-photon fluorescence imaging reveals a Golgi apparatus superoxide anion-mediated hepatic ischaemia-reperfusion signalling pathway

2019 ◽  
Vol 10 (3) ◽  
pp. 879-883 ◽  
Author(s):  
Wen Zhang ◽  
Jiao Zhang ◽  
Ping Li ◽  
Jihong Liu ◽  
Di Su ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Fluorescence Imaging ◽  
Superoxide Anion ◽  
Fluorescence Probe ◽  
Signalling Pathway ◽  
Two Photon ◽  
Ischaemia Reperfusion ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We developed a two-photon fluorescence probe for the dynamic and reversible detection of O2˙− levels in the Golgi apparatus.

Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues

2019 ◽  
Vol 91 (22) ◽  
pp. 14691-14696 ◽  
Author(s):  
Liyan Chen ◽  
Myoung Ki Cho ◽  
Di Wu ◽  
Hwan Myung Kim ◽  
Juyoung Yoon
Keyword(s):  
Fluorescence Probe ◽  
Live Cells ◽  
Two Photon ◽  
Selective Monitoring ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals A Customized Two Photon Fluorescence Imaging Probe Based on 2D scanning MEMS Mirror Including Electrothermal Two-Level-Ladder Dual S-Shaped Actuators

Micromachines ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 704
Author(s):  
Hussein Mehidine ◽  
Min Li ◽  
Jean-Francois Lendresse ◽  
Francoise Bouvet ◽  
Huikai Xie ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Scanning Speed ◽  
Frame Rate ◽  
Outer Diameter ◽  
Small Aperture ◽  
Imaging Probe ◽  
Excitation Beam ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We report the design and characterization of a two-photon fluorescence imaging miniature probe. This customized two-axis scanning probe is dedicated for intraoperative two-photon fluorescence imaging endomicroscopic use and is based on a micro-electro-mechanical system (MEMS) mirror with a high reflectivity plate and two-level-ladder double S-shaped electrothermal bimorph actuators. The fully assembled probe has a total outer diameter of 4 mm including all elements. With a two-lens configuration and a small aperture MEMS mirror, this probe can generate a large optical scan angle of 24° with 4 V drive voltage and can achieve a 450 µm FOV with a 2-fps frame rate. A uniform Pixel Dwell Time and a stable scanning speed along a raster pattern were demonstrated while a 57-fs pulse duration of the excitation beam was measured at the exit of the probe head. This miniature imaging probe will be coupled to a two-photon fluorescence endomicroscope oriented towards clinical use.

Star-Shaped Glycosylated Conjugated Oligomer for Two-Photon Fluorescence Imaging of Live Cells

2011 ◽  
Vol 23 (20) ◽  
pp. 4428-4434 ◽  
Author(s):  
Guan Wang ◽  
Kan-Yi Pu ◽  
Xinhai Zhang ◽  
Kai Li ◽  
Long Wang ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Live Cells ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescent imaging

2021 ◽  
Author(s):  
Ruiyuan Liu ◽  
Yuping Zhou ◽  
Di Zhang ◽  
Genghan He ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
High Fidelity ◽  
Design And Synthesis ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Design and synthesis of near-infrared (NIR) emissive fluorophore for imaging of organelle and photodynamic therapy has received enormous attention. Hence, NIR emissive fluorophore of high-fidelity lysosome targeting, two-photon fluorescence imaging,...

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