scholarly journals Diazaoxatriangulenium: synthesis of reactive derivatives and conjugation to bovine serum albumin

2016 ◽  
Vol 14 (3) ◽  
pp. 1091-1101 ◽  
Author(s):  
Ilkay Bora ◽  
Sidsel A. Bogh ◽  
Martin Rosenberg ◽  
Marco Santella ◽  
Thomas Just Sørensen ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Lifetime ◽  
Bovine Serum

The red emitting, long fluorescence lifetime diazaoxatriangulenium dye DAOTA has been prepared with reactive NHS-ester and maleimide linkers for bioconjugation.

Time-Resolved Fluorescence of Water-Soluble Pyridinium Salt: Sensitive Detection of the Conformational Changes of Bovine Serum Albumin

2016 ◽  
Vol 70 (10) ◽  
pp. 1733-1738 ◽  
Author(s):  
Lei Li ◽  
Hua Yi ◽  
Menghui Jia ◽  
Mengfang Chang ◽  
Zhongneng Zhou ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescent Probe ◽  
Conformational Changes ◽  
Fluorescence Lifetime ◽  
Bovine Serum ◽  
Pyridinium Salt ◽  
Water Soluble ◽  
Time Resolved Fluorescence ◽  
Time Resolved

In this paper, we report a pyridinium salt “turn-on” fluorescent probe, 4-[2-(4-Dimethylamino-phenyl)-vinyl]-1-methylpyridinium iodide (p-DASPMI), and applied its time-resolved fluorescence (TRF) to monitor the protein conformational changes. Both the fluorescence lifetime and quantum yield (QY) of p-DASPMI were increased about two orders of magnitude after binding to the protein bovine serum albumin (BSA). The free p-DASPMI in solution presents an ultrashort fluorescence lifetime (12.4 ps), thus it does not interfere the detection of bound p-DASPMI which has nanosecond fluorescence lifetime. Decay-associated spectra (DAS) show that p-DASPMI molecules bind to subdomains IIA and IIIA of BSA. The TRF decay profiles of p-DASPMI can be described by the multi-exponential decay function ([Formula: see text]), and the obtained parameters, such as lifetimes ([Formula: see text]), fractional amplitudes ([Formula: see text]), and fractional intensities ([Formula: see text]), may be used to deduce the conformational changes of BSA. The pH and Cu2+ induced conformational changes of BSA were investigated through the TRF of p-DASPMI. The results show that the p-DASPMI is a candidate fluorescent probe in studying the conformational changes of proteins through TRF spectroscopy and microscopy in the visible range.

Fluorescence lifetime measurements of native and glycated human serum albumin and bovine serum albumin

1999 ◽  
Author(s):  
Narahari V. Joshi ◽  
Virgina O. d. Joshi ◽  
Silvia Contreras ◽  
Herminia Gil ◽  
Honorio Medina ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Fluorescence Lifetime ◽  
Bovine Serum ◽  
Lifetime Measurements

Tracking structural transitions of bovine serum albumin in surfactant solutions by fluorescence correlation spectroscopy and fluorescence lifetime analysis

Soft Matter ◽  
2015 ◽  
Vol 11 (12) ◽  
pp. 2512-2518 ◽  
Author(s):  
Xuzhu Zhang ◽  
Andrzej Poniewierski ◽  
Sen Hou ◽  
Krzysztof Sozański ◽  
Agnieszka Wisniewska ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Lifetime ◽  
Fluorescence Correlation Spectroscopy ◽  
Bovine Serum ◽  
Structural Changes ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Surfactant Solutions ◽  
Lifetime Analysis

Sudden structural changes of BSA in surfactant solutions are observed from FCS curves.

The binding of bovine serum albumin with dye molecules at different pH values. Fluorescence lifetime studies

Laser Physics ◽  
2021 ◽  
Vol 31 (6) ◽  
pp. 065601
Author(s):  
E S Gorodnichev ◽  
A A Kuleshova ◽  
O I Volkova ◽  
A M Saletsky
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Lifetime ◽  
Bovine Serum ◽  
Dye Molecules ◽  
Ph Values ◽  
Lifetime Studies

scholarly journals Monitoring the endocytosis of bovine serum albumin based on the fluorescence lifetime of small squaraine dye in living cells

2019 ◽  
Vol 11 (1) ◽  
pp. 149 ◽  
Author(s):  
Fangrui Lin ◽  
Pintu Das ◽  
Yihua Zhao ◽  
Binglin Shen ◽  
Rui Hu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Lifetime ◽  
Bovine Serum ◽  
Living Cells ◽  
Squaraine Dye
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