Dual enzyme-responsive “turn-on” fluorescence sensing systems based on in situ formation of 7-hydroxy-2-iminocoumarin scaffolds

2015 ◽  
Vol 13 (41) ◽  
pp. 10348-10361 ◽  
Author(s):  
Sylvain Debieu ◽  
Anthony Romieu
Keyword(s):  
Domino Reactions ◽  
Fluorescence Sensing ◽  
Turn On ◽  
Sensing Systems ◽  
Fluorogenic Probes ◽  
In Situ Formation

We herein report a novel class of dual enzyme-responsive fluorogenic probes based on two orthogonal deprotection reactions via the “covalent assembly” principle. Sensing of two different enzymes (hydrolase and nitroreductase) through domino reactions, producing the push–pull backbone of a fluorescent 3-substituted 7-hydroxy-2-iminocoumarin dye, is reported.

Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots

2021 ◽  
Vol 1157 ◽  
pp. 338394
Author(s):  
Xiao-Yue Tang ◽  
Yi-Ming Liu ◽  
Xiao-Lin Bai ◽  
Hao Yuan ◽  
Yi-Kao Hu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Live Cells ◽  
Turn On ◽  
Dopamine Detection ◽  
In Situ Formation

scholarly journals A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Xiaoxia Liu ◽  
Miaomiao Tian ◽  
Wenmei Gao ◽  
Jinzhong Zhao
Keyword(s):  
Low Cost ◽  
Boronic Acids ◽  
Emission Peak ◽  
Excitation Wavelength ◽  
Fluorescence Sensing ◽  
Serum Samples ◽  
Turn On ◽  
Rapid Reaction

An efficient, sensitive, and low-cost method has been developed for turn-on fluorescence sensing of dopamine (DA). The method relies on the rapid reaction of DA and 3-Hydroxyphenylboronic acid (3-HPBA) via specific recognition between boronic acids and cis-diol of DA in alkaline solution. The reaction product shows an excitation wavelength of 417 nm and the maximum emission peak at 470 nm. The proposed method allows the determination of DA in the range of 50 nM–25 μM, and the whole detection can be completed within 5 minutes. Furthermore, the presented approach has good selectivity and has been successfully applied to DA sensing in human serum samples, showing great potential in clinical diagnosis.

scholarly journals “Turn-On” Protein Fluorescence: In Situ Formation of Cyanine Dyes

2015 ◽  
Vol 137 (3) ◽  
pp. 1073-1080 ◽  
Author(s):  
Ipek Yapici ◽  
Kin Sing Stephen Lee ◽  
Tetyana Berbasova ◽  
Meisam Nosrati ◽  
Xiaofei Jia ◽  
...  
Keyword(s):  
Cyanine Dyes ◽  
Protein Fluorescence ◽  
Turn On ◽  
In Situ Formation

A nuclease-assisted label-free aptasensor for fluorescence turn-on detection of ATP based on the in situ formation of copper nanoparticles

2017 ◽  
Vol 87 ◽  
pp. 760-763 ◽  
Author(s):  
Quanwei Song ◽  
Ruihua Wang ◽  
Feifei Sun ◽  
Hongkun Chen ◽  
Zoumengke Wang ◽  
...  
Keyword(s):  
Copper Nanoparticles ◽  
Label Free ◽  
Turn On ◽  
In Situ Formation ◽  
Fluorescence Turn On

In situ formation of silvernanostructures within a polysaccharide film and application as a potential biocompatible fluorescence sensing medium

Soft Matter ◽  
2012 ◽  
Vol 8 (3) ◽  
pp. 653-659 ◽  
Author(s):  
Graham Hungerford ◽  
Marion Toury ◽  
David McLoskey ◽  
Nicole Donaldson ◽  
A. Sheila Holmes-Smith
Keyword(s):  
Fluorescence Sensing ◽  
In Situ Formation ◽  
Polysaccharide Film

scholarly journals Formation of compact systems of super-Earths via dynamical instabilities and giant impacts

2019 ◽  
Vol 491 (4) ◽  
pp. 5595-5620 ◽  
Author(s):  
Sanson T S Poon ◽  
Richard P Nelson ◽  
Seth A Jacobson ◽  
Alessandro Morbidelli
Keyword(s):  
Initial Conditions ◽  
Post Processing ◽  
Significant Modification ◽  
Kepler Mission ◽  
Giant Impacts ◽  
Low Mass ◽  
In Situ Formation ◽  
Dynamical Instabilities ◽  
Gaseous Envelopes

ABSTRACT The NASA’s Kepler mission discovered ∼700 planets in multiplanet systems containing three or more transiting bodies, many of which are super-Earths and mini-Neptunes in compact configurations. Using N-body simulations, we examine the in situ, final stage assembly of multiplanet systems via the collisional accretion of protoplanets. Our initial conditions are constructed using a subset of the Kepler five-planet systems as templates. Two different prescriptions for treating planetary collisions are adopted. The simulations address numerous questions: Do the results depend on the accretion prescription?; do the resulting systems resemble the Kepler systems, and do they reproduce the observed distribution of planetary multiplicities when synthetically observed?; do collisions lead to significant modification of protoplanet compositions, or to stripping of gaseous envelopes?; do the eccentricity distributions agree with those inferred for the Kepler planets? We find that the accretion prescription is unimportant in determining the outcomes. The final planetary systems look broadly similar to the Kepler templates adopted, but the observed distributions of planetary multiplicities or eccentricities are not reproduced, because scattering does not excite the systems sufficiently. In addition, we find that ∼1 per cent of our final systems contain a co-orbital planet pair in horseshoe or tadpole orbits. Post-processing the collision outcomes suggests that they would not significantly change the ice fractions of initially ice-rich protoplanets, but significant stripping of gaseous envelopes appears likely. Hence, it may be difficult to reconcile the observation that many low-mass Kepler planets have H/He envelopes with an in situ formation scenario that involves giant impacts after dispersal of the gas disc.

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