A fluorescent turn-on H2S-responsive probe: design, synthesis and application

2015 ◽  
Vol 13 (38) ◽  
pp. 9760-9766 ◽  
Author(s):  
Yufeng Zhang ◽  
Haiyan Chen ◽  
Dan Chen ◽  
Di Wu ◽  
Xiaoqiang Chen ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Insulin Secretion ◽  
Ischemia Reperfusion ◽  
Probe Design ◽  
Signaling Molecule ◽  
Design Synthesis ◽  
The Third ◽  
Turn On ◽  
Physiological Processes

Hydrogen sulfide (H2S) is considered as the third signaling moleculein vivoand it plays an important role in various physiological processes and pathological processesin vivo, such as vasodilation, apoptosis, neurotransmission, ischemia/reperfusion-induced injury, insulin secretion and inflammation.

Abstract 843: Cardiomyocyte Overexpression of the Hydrogen Sulfide Producing Enzyme Cystathioine gamma-Lyase Attenuates Myocardial Ischemia-Reperfusion Injury

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
John W Elrod ◽  
John W Calvert ◽  
Chi-Wing Chow ◽  
Joanna Morrison ◽  
Jeannette E Doeller ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Infarct Size ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Fold Increase ◽  
Area At Risk ◽  
Physiological Processes ◽  
Myocardial Ischemia Reperfusion Injury ◽  
Myocardial Ischemia Reperfusion

Background : Hydrogen sulfide (H 2 S) was recently discovered to be an endogenously produced gaseous second messenger capable of modulating many physiological processes. We have previously demonstrated that administration of a H 2 S donor limits the extent of myocardial infarction. This prompted us to investigate the potential of endogenously generated H 2 S in acute cardioprotection utilizing mice with transgenic overexpression of an H 2 S producing enzyme. Methods: Mice with cardiac-specific overexpression of murine cystathionine γ-lyase (αMHC-CGL-Tg) were generated and analyzed for increased enzyme expression and H 2 S production utilizing a H 2 S specific polarographic electrode. αMHC-CGL-Tg and WT mice were then subjected to 45 min of in vivo LCA ischemia and 72 hr reperfusion and infarct size was evaluated using TTC staining. Results: αMHC-CGL-Tg mice displayed an increased level of myocardial CGL RNA, which translated into a (15 fold) increase in protein expression. This increase in CGL enzyme resulted in a significant (2 fold) increase in H 2 S production by myocardial homogenates of αMHC-CGL-Tg mice. αMHC-CGL-Tg mice were found to have a 47% reduction in infarct size per area-at-risk (INF/AAR) as compared to WT littermates. AAR was similar between both groups. Conclusions: This is the first evidence that overexpression of a H 2 S producing enzyme can decrease infarct size following MI-R injury. These findings demonstrate that modulation of endogenous H 2 S production may be of clinical benefit in ischemic disorders and that H 2 S generating enzymes may be viable therapeutic targets.

scholarly journals Physiological Implications of Hydrogen Sulfide in Plants: Pleasant Exploration behind Its Unpleasant Odour

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Zhuping Jin ◽  
Yanxi Pei
Keyword(s):  
Hydrogen Sulfide ◽  
Growth And Development ◽  
Defense Responses ◽  
Plant Responses ◽  
Plant Growth And Development ◽  
Systematic Classification ◽  
Physiological Processes ◽  
Overwhelming Evidence

Recently, overwhelming evidence has proven that hydrogen sulfide (H2S), which was identified as a gasotransmitter in animals, plays important roles in diverse physiological processes in plants as well. With the discovery and systematic classification of the enzymes producing H2Sin vivo, a better understanding of the mechanisms by which H2S influences plant responses to various stimuli was reached. There are many functions of H2S, including the modulation of defense responses and plant growth and development, as well as the regulation of senescence and maturation. Additionally, mounting evidence indicates that H2S signaling interacts with plant hormones, hydrogen peroxide, nitric oxide, carbon monoxide, and other molecules in signaling pathways.

Hydrogen Sulfide, the Third Gaseous Signaling Molecule With Cardiovascular Properties, Is Decreased in Hemodialysis Patients

2010 ◽  
Vol 20 (5) ◽  
pp. S11-S14 ◽  
Author(s):  
Alessandra F. Perna ◽  
Maria Grazia Luciano ◽  
Diego Ingrosso ◽  
Ilaria Raiola ◽  
Paola Pulzella ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Hemodialysis Patients ◽  
Signaling Molecule ◽  
The Third

Therapeutic Metabolic Inhibition: Hydrogen Sulfide Significantly Mitigates Skeletal Muscle Ischemia Reperfusion Injury In Vitro and In Vivo

2010 ◽  
Vol 126 (6) ◽  
pp. 1890-1898 ◽  
Author(s):  
Peter W. Henderson ◽  
Sunil P. Singh ◽  
Andrew L. Weinstein ◽  
Vijay Nagineni ◽  
Daniel C. Rafii ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Hydrogen Sulfide ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Metabolic Inhibition ◽  
Muscle Ischemia

scholarly journals Hydrogen sulfide triggers late-phase preconditioning in postischemic small intestine by an NO- and p38 MAPK-dependent mechanism

2009 ◽  
Vol 296 (3) ◽  
pp. H868-H876 ◽  
Author(s):  
Mozow Yusof ◽  
Kazuhiro Kamada ◽  
Theodore Kalogeris ◽  
F. Spencer Gaskin ◽  
Ronald J. Korthuis
Keyword(s):  
Hydrogen Sulfide ◽  
P38 Mapk ◽  
Ischemia Reperfusion ◽  
Late Phase ◽  
Mitogen Activated Protein Kinase ◽  
Sodium Hydrosulfide ◽  
Pharmacological Inhibition ◽  
Mapk Inhibitors ◽  
Dependent Mechanism

Hydrogen sulfide (H2S) is one of three endogenous gases, along with carbon monoxide (CO) and nitric oxide (NO), that exert a variety of important vascular actions in vivo. Although it has been demonstrated that CO or NO can trigger the development of a preconditioned phenotype in postischemic tissues, it is unclear whether H2S may also induce protection in organs subsequently exposed to ischemia-reperfusion (I/R). In light of these observations, we postulated that preconditioning with the exogenous H2S donor sodium hydrosulfide (NaHS-PC) would inhibit leukocyte rolling (LR) and adhesion (LA) induced by I/R. We used intravital microscopic techniques to demonstrate that NaHS-PC 24 h, but not 1 h, before I/R causes postcapillary venules to shift to an anti-inflammatory phenotype in wild-type (WT) mice such that these vessels fail to support LR and LA during reperfusion. The protective effect of NaHS-PC on LR was largely abolished by coincident pharmacological inhibition of NO synthase (NOS) in WT animals and was absent in endothelial NOS-deficient (eNOS−/−) mice. A similar pattern of response was noted in WT mice treated concomitantly with NaHS plus p38 mitogen-activated protein kinase (MAPK) inhibitors (SB 203580 or SK-86002). Whereas the reduction in LA induced by antecedent NaHS was attenuated by pharmacological inhibition of NOS or p38 MAPK in WT mice, the antiadhesive effect of NaHS was still evident in eNOS−/− mice. Thus NaHS-PC prevents LR and LA by triggering the activation of an eNOS- and p38 MAPK-dependent mechanism. However, the role of eNOS in the antiadhesive effect of NaHS-PC was less prominent than its effect to reduce LR.

scholarly journals Hydrogen Sulfide Promotes Nodulation and Nitrogen Fixation in Soybean–Rhizobia Symbiotic System

2019 ◽  
Vol 32 (8) ◽  
pp. 972-985 ◽  
Author(s):  
Hang Zou ◽  
Ni-Na Zhang ◽  
Qing Pan ◽  
Jian-Hua Zhang ◽  
Juan Chen ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Hydrogen Sulfide ◽  
Glutamate Synthase ◽  
Marker Genes ◽  
Symbiotic System ◽  
Signaling Molecule ◽  
Sodium Hydrosulfide ◽  
Physiological Processes ◽  
Soybean Rhizobia ◽  
Early Nodulin

The rhizobium–legume symbiotic system is crucial for nitrogen cycle balance in agriculture. Hydrogen sulfide (H2S), a gaseous signaling molecule, may regulate various physiological processes in plants. However, whether H2S has regulatory effect in this symbiotic system remains unknown. Herein, we investigated the possible role of H2S in the symbiosis between soybean (Glycine max) and rhizobium (Sinorhizobium fredii). Our results demonstrated that an exogenous H2S donor (sodium hydrosulfide [NaHS]) treatment promoted soybean growth, nodulation, and nitrogenase (Nase) activity. Western blotting analysis revealed that the abundance of Nase component nifH was increased by NaHS treatment in nodules. Quantitative real-time polymerase chain reaction data showed that NaHS treatment upregulated the expressions of symbiosis-related genes nodA, nodC, and nodD of S. fredii. In addition, expression of soybean nodulation marker genes, including early nodulin 40 (GmENOD40), ERF required for nodulation (GmERN), nodulation signaling pathway 2b (GmNSP2b), and nodulation inception genes (GmNIN1a, GmNIN2a, and GmNIN2b), were upregulated. Moreover, the expressions of glutamate synthase (GmGOGAT), asparagine synthase (GmAS), nitrite reductase (GmNiR), ammonia transporter (GmSAT1), leghemoglobin (GmLb), and nifH involved in nitrogen metabolism were upregulated in NaHS-treated soybean roots and nodules. Together, our results suggested that H2S may act as a positive signaling molecule in the soybean–rhizobia symbiotic system and enhance the system’s nitrogen fixation ability.

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