A switchable DNA origami nanochannel for regulating molecular transport at the nanometer scale

Nanoscale ◽  
2016 ◽  
Vol 8 (7) ◽  
pp. 3944-3948 ◽  
Author(s):  
Dianming Wang ◽  
Yiyang Zhang ◽  
Miao Wang ◽  
Yuanchen Dong ◽  
Chao Zhou ◽  
...  
Keyword(s):  
Dna Nanotechnology ◽  
Molecular Transport ◽  
Dna Origami ◽  
Nanometer Scale

A nanochannel with a shutter at one end was built by DNA nanotechnology.

scholarly journals Dynamic DNA Origami Devices: from Strand-Displacement Reactions to External-Stimuli Responsive Systems

2018 ◽  
Vol 19 (7) ◽  
pp. 2114 ◽  
Author(s):  
Heini Ijäs ◽  
Sami Nummelin ◽  
Boxuan Shen ◽  
Mauri Kostiainen ◽  
Veikko Linko
Keyword(s):  
Dna Sequences ◽  
Rapid Evolution ◽  
Dna Nanotechnology ◽  
Dna Origami ◽  
Dna Assembly ◽  
Stimuli Responsive ◽  
Nanoscale Structures ◽  
Displacement Reactions ◽  
Materials Research ◽  
External Stimuli

DNA nanotechnology provides an excellent foundation for diverse nanoscale structures that can be used in various bioapplications and materials research. Among all existing DNA assembly techniques, DNA origami proves to be the most robust one for creating custom nanoshapes. Since its invention in 2006, building from the bottom up using DNA advanced drastically, and therefore, more and more complex DNA-based systems became accessible. So far, the vast majority of the demonstrated DNA origami frameworks are static by nature; however, there also exist dynamic DNA origami devices that are increasingly coming into view. In this review, we discuss DNA origami nanostructures that exhibit controlled translational or rotational movement when triggered by predefined DNA sequences, various molecular interactions, and/or external stimuli such as light, pH, temperature, and electromagnetic fields. The rapid evolution of such dynamic DNA origami tools will undoubtedly have a significant impact on molecular-scale precision measurements, targeted drug delivery and diagnostics; however, they can also play a role in the development of optical/plasmonic sensors, nanophotonic devices, and nanorobotics for numerous different tasks.

scholarly journals Molecular Transport through Large Diameter DNA Origami Channels

2017 ◽  
Vol 112 (3) ◽  
pp. 416a ◽  
Author(s):  
Swati Krishnan ◽  
Friedrich Simmel
Keyword(s):  
Large Diameter ◽  
Molecular Transport ◽  
Dna Origami

scholarly journals DNA nanotechnology approaches for microRNA detection and diagnosis

2019 ◽  
Vol 47 (20) ◽  
pp. 10489-10505 ◽  
Author(s):  
Arun Richard Chandrasekaran ◽  
Jibin Abraham Punnoose ◽  
Lifeng Zhou ◽  
Paromita Dey ◽  
Bijan K Dey ◽  
...  
Keyword(s):  
Dna Nanotechnology ◽  
Dna Origami ◽  
Great Promise ◽  
Dna Nanostructures ◽  
New Class ◽  
Advantages And Disadvantages ◽  
Critical Comparison ◽  
Microrna Detection ◽  
Disease Diagnostics ◽  
Detection And Diagnosis

Abstract MicroRNAs are involved in the crucial processes of development and diseases and have emerged as a new class of biomarkers. The field of DNA nanotechnology has shown great promise in the creation of novel microRNA biosensors that have utility in lab-based biosensing and potential for disease diagnostics. In this Survey and Summary, we explore and review DNA nanotechnology approaches for microRNA detection, surveying the literature for microRNA detection in three main areas of DNA nanostructures: DNA tetrahedra, DNA origami, and DNA devices and motifs. We take a critical look at the reviewed approaches, advantages and disadvantages of these methods in general, and a critical comparison of specific approaches. We conclude with a brief outlook on the future of DNA nanotechnology in biosensing for microRNA and beyond.

scholarly journals Programming Structured DNA Assemblies to Probe Biophysical Processes

2019 ◽  
Vol 48 (1) ◽  
pp. 395-419 ◽  
Author(s):  
Eike-Christian Wamhoff ◽  
James L. Banal ◽  
William P. Bricker ◽  
Tyson R. Shepherd ◽  
Molly F. Parsons ◽  
...  
Keyword(s):  
Structural Biology ◽  
Energy Transport ◽  
Dna Nanotechnology ◽  
Dna Origami ◽  
Design And Synthesis ◽  
Copy Numbers ◽  
Target Nucleic Acid ◽  
Biophysical Processes ◽  
Fully Automatic ◽  
Structural Dna Nanotechnology

Structural DNA nanotechnology is beginning to emerge as a widely accessible research tool to mechanistically study diverse biophysical processes. Enabled by scaffolded DNA origami in which a long single strand of DNA is weaved throughout an entire target nucleic acid assembly to ensure its proper folding, assemblies of nearly any geometric shape can now be programmed in a fully automatic manner to interface with biology on the 1–100-nm scale. Here, we review the major design and synthesis principles that have enabled the fabrication of a specific subclass of scaffolded DNA origami objects called wireframe assemblies. These objects offer unprecedented control over the nanoscale organization of biomolecules, including biomolecular copy numbers, presentation on convex or concave geometries, and internal versus external functionalization, in addition to stability in physiological buffer. To highlight the power and versatility of this synthetic structural biology approach to probing molecular and cellular biophysics, we feature its application to three leading areas of investigation: light harvesting and nanoscale energy transport, RNA structural biology, and immune receptor signaling, with an outlook toward unique mechanistic insight that may be gained in these areas in the coming decade.

scholarly journals An electrochemical biosensor exploiting binding-induced changes in electron transfer of electrode-attached DNA origami to detect hundred nanometer-scale targets

Nanoscale ◽  
2020 ◽  
Vol 12 (26) ◽  
pp. 13907-13911 ◽  
Author(s):  
Netzahualcóyotl Arroyo-Currás ◽  
Muaz Sadeia ◽  
Alexander K. Ng ◽  
Yekaterina Fyodorova ◽  
Natalie Williams ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Electrochemical Biosensor ◽  
Direct Detection ◽  
Dna Origami ◽  
Nanometer Scale ◽  
Recognition Element ◽  
Induced Changes

Using DNA origami as the recognition element in an electrochemical biosensor enables the selective and direct detection of “mesoscale” virus-sized analytes.

Programmed Nanopatterning of Organic/Inorganic Nanoparticles Using Nanometer-Scale Wells Embedded in a DNA Origami Scaffold

Small ◽  
2010 ◽  
Vol 6 (23) ◽  
pp. 2664-2667 ◽  
Author(s):  
Akinori Kuzuya ◽  
Naohiro Koshi ◽  
Mayumi Kimura ◽  
Kentaro Numajiri ◽  
Takahiro Yamazaki ◽  
...  
Keyword(s):  
Dna Origami ◽  
Inorganic Nanoparticles ◽  
Nanometer Scale

scholarly journals Complex multicomponent patterns rendered on a 3D DNA-barrel pegboard

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Shelley F. J. Wickham ◽  
Alexander Auer ◽  
Jianghong Min ◽  
Nandhini Ponnuswamy ◽  
Johannes B. Woehrstein ◽  
...  
Keyword(s):  
Dna Nanotechnology ◽  
Super Resolution ◽  
Dna Origami ◽  
Design And Optimization ◽  
Hierarchical Assembly ◽  
Double Helices ◽  
Specialist Groups ◽  
Parallel Arrays ◽  
Super Resolution Microscopy ◽  
Rhombic Lattice

AbstractDNA origami, in which a long scaffold strand is assembled with a many short staple strands into parallel arrays of double helices, has proven a powerful method for custom nanofabrication. However, currently the design and optimization of custom 3D DNA-origami shapes is a barrier to rapid application to new areas. Here we introduce a modular barrel architecture, and demonstrate hierarchical assembly of a 100 megadalton DNA-origami barrel of ~90 nm diameter and ~250 nm height, that provides a rhombic-lattice canvas of a thousand pixels each, with pitch of ~8 nm, on its inner and outer surfaces. Complex patterns rendered on these surfaces were resolved using up to twelve rounds of Exchange-PAINT super-resolution microscopy. We envision these structures as versatile nanoscale pegboards for applications requiring complex 3D arrangements of matter, which will serve to promote rapid uptake of this technology in diverse fields beyond specialist groups working in DNA nanotechnology.

DNA Origami Nanoplate-Based Emulsion with Designed Nanopore Function

2018 ◽  
Author(s):  
Daisuke Ishikawa ◽  
Yuki Suzuki ◽  
Chikako Kurokawa ◽  
Masayuki Ohara ◽  
Misato Tsuchiya ◽  
...  
Keyword(s):  
Shape Control ◽  
Dna Nanotechnology ◽  
Dna Origami ◽  
Cell Engineering ◽  
General Strategy ◽  
Surface Chemical ◽  
Artificial Cell ◽  
Physical Chemical ◽  
Oil Water ◽  
Molecular Robots

Bio-inspired functional microcapsules stabilised with surfactants, copolymers, and nano/microparticles have attracted much attention in many fields from physical/chemical science to artificial cell engineering. Although the particle-stabilized microcapsules have advantages for their stability and rich ways for functionalisation such as surface chemical modifications and shape control of particles, versatile methods for their designable functionalisation are desired to expand their possibilities. Here, we report a DNA-based microcapsule composed of a water-in-oil microdroplet stabilised with amphiphilised DNA origami nanoplates. By utilising function programmability achieved by DNA nanotechnology, the DNA nanoplates were designed as a nanopore device for ion transportation as well as the interface stabiliser. Microscopic observations showed that the microcapsule formed by amphiphilic DNA nanoplates accumulated at the oil-water interface. Ion current measurements demonstrated that pores in the nanoplates functioned as ion channels. These findings provide a general strategy for programmable designing of microcapsules for engineering artificial cells and molecular robots.<br>

scholarly journals Recent Advances in Novel DNA Guiding Nanofabrication and Nanotechnology

2018 ◽  
Vol 4 (1) ◽  
pp. 32-52 ◽  
Author(s):  
Zhiguang Suo ◽  
Jingqi Chen ◽  
Ziheng Hu ◽  
Yihao Liu ◽  
Feifei Xing ◽  
...  
Keyword(s):  
Dna Recognition ◽  
Genetic Material ◽  
Dna Nanotechnology ◽  
Structural Features ◽  
Dna Origami ◽  
Site Specific ◽  
Metal Organic ◽  
Nanomaterials Synthesis ◽  
Promising Development ◽  
3D Topology

Abstract DNA as life’s genetic material has been widely investigated around the world. In recent years, with the fiery researches on nanomaterials, it also plays an important role in the development of material science due to its extraordinary molecular recognition capability and prominent structural features. In this mini review, we mainly overview the recent progresses of DNA guiding self-assembled nanostructures and nanofabrication. Typical DNA tile-based assembly and DNA origami nanotechnologies are presented, utilizing the recent 3D topology methods to fabricate multidimensional structures with unique properties. Then the site-specific nanomaterials synthesis and nano-DNA recognition on different DNA scaffolds/templates are demonstrated with excellent addressability, biocompatibility and structural programmability. Various nanomaterials, such as metals, carbon family materials, quantum dots, metal-organic frameworks, and DNA-based liquid crystals are briefly summarized. Finally, the present limitation and future promising development directions are discussed in conclusion and perspective. We wish this review would provide useful information toward the broader scientific interests in DNA nanotechnology.

Precisely Programmed and Robust 2D Streptavidin Nanoarrays by Using Periodical Nanometer-Scale Wells Embedded in DNA Origami Assembly

ChemBioChem ◽  
2009 ◽  
Vol 10 (11) ◽  
pp. 1811-1815 ◽  
Author(s):  
Akinori Kuzuya ◽  
Mayumi Kimura ◽  
Kentaro Numajiri ◽  
Naohiro Koshi ◽  
Toshiyuki Ohnishi ◽  
...  
Keyword(s):  
Dna Origami ◽  
Nanometer Scale
Sign in / Sign up

Export Citation Format

Share Document