scholarly journals Virosome engineering of colloidal particles and surfaces: bioinspired fusion to supported lipid layers

Nanoscale ◽  
2016 ◽  
Vol 8 (15) ◽  
pp. 7933-7941 ◽  
Author(s):  
J. Fleddermann ◽  
E. Diamanti ◽  
S. Azinas ◽  
M. Košutić ◽  
L. Dähne ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Cell Membranes ◽  
Colloidal Particles ◽  
Supported Lipid Bilayers ◽  
Virus Fusion ◽  
Ph Dependent ◽  
Lipid Layers ◽  
Dependent Mechanism

Inmunostimulating reconstituted influenza virosomes (IRIVs), liposomes with functional viral envelop glycoproteins, fuse on supported lipid bilayers with a pH dependent mechanism, in analogy to the virus fusion on cell membranes.

Mimicking Influenza Virus Fusion Using Supported Lipid Bilayers

Langmuir ◽  
2014 ◽  
Vol 30 (38) ◽  
pp. 11394-11400 ◽  
Author(s):  
Cédric Godefroy ◽  
Selma Dahmane ◽  
Patrice Dosset ◽  
Olivier Adam ◽  
Marie-Claire Nicolai ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Lipid Bilayers ◽  
Supported Lipid Bilayers ◽  
Virus Fusion

Preserved Transmembrane Protein Mobility in Polymer-Supported Lipid Bilayers Derived from Cell Membranes

2015 ◽  
Vol 87 (18) ◽  
pp. 9194-9203 ◽  
Author(s):  
Hudson Pace ◽  
Lisa Simonsson Nyström ◽  
Anders Gunnarsson ◽  
Elizabeth Eck ◽  
Christopher Monson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Cell Membranes ◽  
Transmembrane Protein ◽  
Supported Lipid Bilayers ◽  
Protein Mobility ◽  
Polymer Supported

Molecular diffusion and nano-mechanical properties of multi-phase supported lipid bilayers

2019 ◽  
Vol 21 (30) ◽  
pp. 16686-16693 ◽  
Author(s):  
Tatsuhiro Maekawa ◽  
Hokyun Chin ◽  
Takashi Nyu ◽  
Tun Naw Sut ◽  
Abdul Rahim Ferhan ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Lipid Bilayers ◽  
Cell Membranes ◽  
Molecular Diffusion ◽  
Supported Lipid Bilayers ◽  
Multi Phase ◽  
Applied Biology

Understanding the properties of cell membranes is important in the fields of fundamental and applied biology.

scholarly journals Atomic Force Microscopy to Characterize Antimicrobial Peptide-Induced Defects in Model Supported Lipid Bilayers

2021 ◽  
Vol 9 (9) ◽  
pp. 1975
Author(s):  
Kathleen W. Swana ◽  
Ramanathan Nagarajan ◽  
Terri A. Camesano
Keyword(s):  
Atomic Force Microscopy ◽  
Amino Acid ◽  
Lipid Bilayers ◽  
Mammalian Cell ◽  
Cell Membranes ◽  
Supported Lipid Bilayers ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging ◽  
Over Time

Antimicrobial peptides (AMPs) interact with bacterial cell membranes through a variety of mechanisms, causing changes extending from nanopore formation to microscale membrane lysis, eventually leading to cell death. Several AMPs also disrupt mammalian cell membranes, despite their significantly different lipid composition and such collateral hemolytic damage hinders the potential therapeutic applicability of the AMP as an anti-microbial. Elucidating the mechanisms underlying the AMP–membrane interactions is challenging due to the variations in the chemical and structural features of the AMPs, the complex compositional variations of cell membranes and the inadequacy of any single experimental technique to comprehensively probe them. (1) Background: Atomic Force Microscopy (AFM) imaging can be used in combination with other techniques to help understand how AMPs alter the orientation and structural organization of the molecules within cell membranes exposed to AMPs. The structure, size, net charge, hydrophobicity and amphipathicity of the AMPs affect how they interact with cell membranes of differing lipid compositions. (2) Methods: Our study examined two different types of AMPs, a 20-amino acid, neutral, α-helical (amphipathic) peptide, alamethicin, and a 13-amino acid, non-α-helical cationic peptide, indolicidin (which intramolecularly folds, creating a hydrophobic core), for their interactions with supported lipid bilayers (SLBs). Robust SLB model membranes on quartz supports, incorporating predominantly anionic lipids representative of bacterial cells, are currently not available and remain to be developed. Therefore, the SLBs of zwitterionic egg phosphatidylcholine (PC), which represents the composition of a mammalian cell membrane, was utilized as the model membrane. This also allows for a comparison with the results obtained from the Quartz Crystal Microbalance with Dissipation (QCM-D) experiments conducted for these peptides interacting with the same zwitterionic SLBs. Further, in the case of alamethicin, because of its neutrality, the lipid charge may be less relevant for understanding its membrane interactions. (3) Results: Using AFM imaging and roughness analysis, we found that alamethicin produced large, unstable defects in the membrane at 5 µM concentrations, and completely removed the bilayer at 10 µM. Indolicidin produced smaller holes in the bilayer at 5 and 10 µM, although they were able to fill in over time. The root-mean-square (RMS) roughness values for the images showed that the surface roughness caused by visible defects peaked after peptide injection and gradually decreased over time. (4) Conclusions: AFM is useful for helping to uncover the dynamic interactions between different AMPs and cell membranes, which can facilitate the selection and design of more efficient AMPs for use in therapeutics and antimicrobial applications.

Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Supported Lipid Bilayer ◽  
Head Groups ◽  
Lipid Diffusion ◽  
Kinetics Of ◽  
Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

scholarly journals Silica nanoparticle supported lipid bilayers for gene delivery

2009 ◽  
pp. 5100 ◽  
Author(s):  
Juewen Liu ◽  
Alison Stace-Naughton ◽  
C. Jeffrey Brinker
Keyword(s):  
Gene Delivery ◽  
Lipid Bilayers ◽  
Silica Nanoparticle ◽  
Supported Lipid Bilayers

scholarly journals Formation of Supported Lipid Bilayers Derived from Vesicles of Various Compositional Complexity on Conducting Polymer/Silica Substrates

Langmuir ◽  
2021 ◽  
Author(s):  
Hanna Ulmefors ◽  
Josefin Nissa ◽  
Hudson Pace ◽  
Olov Wahlsten ◽  
Anders Gunnarsson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Conducting Polymer ◽  
Supported Lipid Bilayers ◽  
Silica Substrates
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