Ultrafast colorimetric determination of predominant protein structure evolution with gold nanoplasmonic particles

Nanoscale ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 1952-1959 ◽  
Author(s):  
Hye Young Kim ◽  
Inhee Choi

We report an ultrafast colorimetric method for the real-time monitoring of protein structure evolution and the determination of predominant structuresviananoparticle-assisted protein aggregation.

Nanoscale ◽  
2015 ◽  
Vol 7 (13) ◽  
pp. 5719-5725 ◽  
Author(s):  
Ruixue Duan ◽  
Boya Wang ◽  
Fan Hong ◽  
Tianchi Zhang ◽  
Yongmei Jia ◽  
...  

We report a simple colorimetric method using gold nanoparticles as signal generators for the real-time detection of the product of the strand displacement cascade.


1990 ◽  
Vol 73 (1) ◽  
pp. 54-57 ◽  
Author(s):  
Kurt Kolar

Abstract A colorimetric method for the determination of hydroxyproline as a measure of collagen in meat and meat products has been collaboratively studied in 18 laboratories. The method includes hydrolysis with sulfuric acid, oxidation with chloramine- T, and formation of a reddish purple complex with 4- dimethylaminobenzaldehyde. Five frozen and 3 freeze-dried samples were tested, ranging in content from 0.11 to 0.88% and from 0.39 to 4.0% hydroxyproline, respectively. The mean values of 2 identical samples were 0.245 and 0.251 %. The average recovery from a spiked sample was 96.1 %. The hydroxyproline content of a known sample (a mixture of 2 samples in the ratio 5:2) was calculated to 1.42%, which agrees well with the analytical result, 1.40%. In comparison with other collaborative studies, based on the ISO analytical method, the repeatability and reproducibility of this method agree well with the other results. This method was accepted as an official NMKL method by all national Committees, and has been adopted official first action by AOAC as an NMKLAOAC method.


1938 ◽  
Vol 9 (3) ◽  
pp. 336-338 ◽  
Author(s):  
E. Aschaffenburg

It has been repeatedly pointed out(1, 2, 3) that the properties of cheese during the different stages of its manufacture should be correlated with the hydrogenion concentration rather than with the titratable acidity. Little systematic work has, however, so far been carried out in this direction, except for a study of the relationship between pH and titratable acidity in Cheddar cheese by Brown & Price(4). In planning work on similar lines, it was realized that the potentiometric methods of determining pH require expensive equipment and skilled attention, so that a supplementary colorimetric method, if sufficiently accurate to indicate the major changes in pH, should appeal more strongly to the practical cheesemaker on account of its cheapness and simplicity and the ease with which the outfit can be transported.


2017 ◽  
Vol 19 (26) ◽  
pp. 17187-17198 ◽  
Author(s):  
Marshall R. Ligare ◽  
Grant E. Johnson ◽  
Julia Laskin

Real-time monitoring of the gold cluster synthesis by electrospray ionization mass spectrometry reveals distinct formation pathways for Au8, Au9 and Au10 clusters.


Author(s):  
Neng Huang ◽  
Junxing Zhu ◽  
Chaonian Guo ◽  
Shuhan Cheng ◽  
Xiaoyong Li

With the rapid development of mobile Internet, there is a higher demand for the real-time, reliability and availability of information systems and to prevent the possible systemic risks of information systems, various business consistency standards and regulatory guidelines have been published, such as Recovery Time Object (RTO) and Recovery Point Object (RPO). Some of the current related researches focus on the standards, methods, management tools and technical frameworks of business consistency, while others study the data consistency algorithms in the cases of large data, cloud computing and distributed storage. However, few researchers have studied on how to monitor the data consistency and RPO of production-disaster recovery, and what architecture and technology should be applied in the monitoring. Moreover, in some information systems, due to the complex structures and distributions of data, it is difficult for traditional methods to quickly detect and accurately locate the first error data. Besides, due to the separation of production data center (PDC) and disaster recovery data center (DRDC), it is difficult to calculate the data difference and RPO between the two centers. This paper first discusses the architecture of remote distributed DRDCs. The architecture can make the disaster recovery (DR) system always online and the data always readable, and support the real-time monitoring of data availability, consistency as well as other related indicators, in this way to make DRDC out-of-the-box in disasters. Second, inspired by blockchain, this paper proposes a method to realize real-time monitoring of data consistency and RTO by building hash chains for PDC and DRDC. Third, this paper evaluates the hash chain operations from the algorithm time complexity, the data consistency, and the validity of RPO monitoring algorithms and since DR system is actually a kind of distributed system, the proposed approach can also be applied to the data consistency detection and data difference monitoring in other distributed systems.


1987 ◽  
Vol 70 (3) ◽  
pp. 568-571 ◽  
Author(s):  
Mohamed E El-Sadek ◽  
Hisham E Abdel Latef ◽  
Afaf A Aboul Khier

Abstract A colorimetric method is proposed for determination of terbutaline sulfate, orciprenaline sulfate, and their dosage forms. The suggested method depends on nitrosation of the 2 drugs by using sodium nitrite and hydrochloric acid. Addition of sodium hydroxide increases the intensity of the color developed. The difference between absorption values measured in acid and alkaline media is taken as a measure of concentration. Variables were carefully studied and optimized. Results for both compounds adhered to Beer's law over the range 2- 28 μg/mL. The method has proved to be accurate and precise for analysis of pharmaceutical dosage forms.


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