Real-time investigation of human topoisomerase I reaction kinetics using an optical sensor: a fast method for drug screening and determination of active enzyme concentrations

Nanoscale ◽  
2015 ◽  
Vol 7 (21) ◽  
pp. 9825-9834 ◽  
Author(s):  
Emil L. Kristoffersen ◽  
Line A. Jørgensen ◽  
Oskar Franch ◽  
Michael Etzerodt ◽  
Rikke Frøhlich ◽  
...  
Keyword(s):  
Reaction Kinetics ◽  
Drug Screening ◽  
Optical Sensor ◽  
Topoisomerase I ◽  
Fast Method ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Human Dna ◽  
Human Dna Topoisomerase I

Human DNA topoisomerase I (hTopI) is a nuclear enzyme that catalyzes relaxation of super helical tension that arises in the genome during essential DNA metabolic processes.

Isodiospyrin as a novel human DNA topoisomerase I inhibitor

2003 ◽  
Vol 66 (10) ◽  
pp. 1981-1991 ◽  
Author(s):  
Chun-Yuan Ting ◽  
Chia-Tse Hsu ◽  
Hsiang-Ting Hsu ◽  
Jin-Shan Su ◽  
Tzong-Yueh Chen ◽  
...  
Keyword(s):  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Topoisomerase I Inhibitor ◽  
Human Dna ◽  
Human Dna Topoisomerase I

Synthesis, crystal structure, DNA interaction and in vitro anticancer activity of a Cu(ii) complex of purpurin: dual poison for human DNA topoisomerase I and II

RSC Advances ◽  
2014 ◽  
Vol 4 (103) ◽  
pp. 59344-59357 ◽  
Author(s):  
Piyal Das ◽  
Chetan Kumar Jain ◽  
Sanjoy K. Dey ◽  
Rajat Saha ◽  
Abhishek Dutta Chowdhury ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Topoisomerase I ◽  
Dna Interaction ◽  
Dna Topoisomerase I ◽  
Oxygen Species ◽  
Dna Topoisomerase ◽  
Human Dna ◽  
Anti Tumor Activity ◽  
Human Dna Topoisomerase I

Although generation of reactive oxygen species (ROS) by anthracycline anticancer drugs is essential for anti-tumor activity, they make these drugs cardiotoxic.

Rapid and prolonged stalling of human DNA topoisomerase I in UVA-irradiated genomic areas

DNA Repair ◽  
2007 ◽  
Vol 6 (12) ◽  
pp. 1757-1763 ◽  
Author(s):  
Christian Mielke ◽  
Faiza M. Kalfalah ◽  
Morten O. Christensen ◽  
Fritz Boege
Keyword(s):  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Human Dna ◽  
Human Dna Topoisomerase I
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