A comprehensive classification of solvent systems used for natural product purifications in countercurrent and centrifugal partition chromatography

2015 ◽  
Vol 32 (11) ◽  
pp. 1556-1561 ◽  
Author(s):  
Krystyna Skalicka-Woźniak ◽  
Ian Garrard
Keyword(s):  
Natural Product ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Solvent Systems ◽  
Comprehensive Classification

This review is a comprehensive classification of solvent systems used for natural product purifications in countercurrent and centrifugal partition chromatography.

Use of centrifugal partition chromatography for assessing partition coefficients in various solvent systems

1989 ◽  
Vol 469 ◽  
pp. 91-99 ◽  
Author(s):  
Nabil El Tayar ◽  
Andrew Marston ◽  
Antoine Bechalany ◽  
Kurt Hostettmann ◽  
Bernard Testa
Keyword(s):  
Partition Coefficients ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Solvent Systems

scholarly journals Target-Guided Isolation of O-tigloylcyclovirobuxeine-B from Buxus sempervirens L. by Centrifugal Partition Chromatography

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4804
Author(s):  
Lara U. Szabó ◽  
Thomas J. Schmidt
Keyword(s):  
Natural Product ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Target Compound ◽  
Preparative Scale ◽  
Buxus Sempervirens ◽  
Life Threatening ◽  
Identification And Characterization

The increasing drug resistance of malaria parasites challenges the treatment of this life-threatening disease. Consequently, the development of innovative and effective antimalarial drugs is inevitable. O-tigloylcyclovirobuxeine-B, a nor-cycloartane alkaloid from Buxussempervirens L., has shown promising and selective in vitro activity in previous studies against Plasmodiumfalciparum (Pf), causative agent of Malaria tropica. For further investigations, it is indispensable to develop an advanced and efficient isolation procedure of this valuable natural product. Accordingly, we used liquid–liquid chromatography including centrifugal partition chromatography (CPC) to obtain the pure alkaloid on a semi-preparative scale. Identification and characterization of the target compound was accomplished by UHPLC/+ESI-QqTOF-MS/MS, 1H NMR and 13C NMR. In conclusion, this work provides a new and efficient method to obtain O-tigloylcyclovirobuxeine-B, a valuable natural product, as a promising antiplasmodial lead structure for the development of innovative and safe medicinal agents.

scholarly journals Preparative Purification of Delphinidin 3-0-sambubioside from Roselle (Hibiscus sabdariffa L.) Petals by fast Centrifugation Partition Chromatography

2010 ◽  
Vol 6 (2) ◽  
pp. 999-1004
Author(s):  
Hilaire Tanoh Kouakou ◽  
Laurent Kouakou Kouakou ◽  
Alain Decendit ◽  
Alain Badoc ◽  
Gregory Da-Costa ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Amyloid Fibrils ◽  
Hibiscus Sabdariffa ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Preparative Scale ◽  
Mobile Phases ◽  
Chromatographic Procedure ◽  
Solvent Systems ◽  
Liquid Chromatographic

Delphinidin 3-0-sambubioside, a Hibiscus anthocyanin, was isolated from MeOH/TFA dried flower of H. sabdariffa. Its purification on preparative scale was obtained by centrifugal partition chromatography (CPC) using the ternary biphasic solvent systems composed of ethyl acetate/1-butanol/water, acidified by 0.1% of TFA. Stationary phase was ethyl acetate/1-butanol/water (5:5:90; v/v). We tested 4 mobile phases and found that the system acetate/1-butanol/water (40:46:14; v/v) was the best to separate anthocyanin mentioned above. This support-free liquid-liquid chromatographic procedure made it possible to isolate delphinidin 3-0-sambubioside from flower of H. sabdariffa. The antiamyloidogenic activity of the isolated stilbenes was evaluated versus b-amyloid fibrils. delphinidin 3-0-sambubioside was found to be active with 67% inhibition at 10 mM.

Characterization of Proanthocyanidin-Rich Dentin Biomodifiers from Plants by Centrifugal Partition Chromatography

Planta Medica ◽  
2013 ◽  
Vol 79 (10) ◽  
Author(s):  
JW Nam ◽  
RS Phansalkar ◽  
JG Napolitano ◽  
SN Chen ◽  
JB McAlpine ◽  
...  
Keyword(s):  
Partition Chromatography ◽  
Centrifugal Partition Chromatography

One-step preparative isolation of aristolochic acids by strong ion-exchange centrifugal partition chromatography

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
AA Abdelgadir ◽  
L Boudesocque-Delaye ◽  
I Thery-Koné ◽  
A Gueiffier ◽  
EM Ahmed ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Preparative Isolation ◽  
Aristolochic Acids ◽  
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