Rhodamine-modified upconversion nanoprobe for distinguishing Cu2+ from Hg2+ and live cell imaging

2016 ◽  
Vol 40 (4) ◽  
pp. 3543-3551 ◽  
Author(s):  
Yanxia Xu ◽  
Huifang Li ◽  
Xianfu Meng ◽  
Jinliang Liu ◽  
Lining Sun ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Mesoporous Silica ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Live Cell ◽  
Upconversion Nanoparticles ◽  
Inorganic Hybrid ◽  
Hybrid Nanoprobe

A new organic–inorganic hybrid nanoprobe based on luminescence resonance energy transfer (LRET) from mesoporous silica coated upconversion nanoparticles to a rhodamine B derivative was prepared for distinguishing Cu2+ from Hg2+ and live cell imaging applications.

Dye selection for live cell imaging of intact siRNA

2012 ◽  
Vol 393 (1-2) ◽  
pp. 23-35 ◽  
Author(s):  
Markus Hirsch ◽  
Dennis Strand ◽  
Mark Helm
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Time Lapse ◽  
Live Cell ◽  
High Yield ◽  
Ph Variation ◽  
Rnai Efficiency

Abstract Investigations into the fate of small interfering RNA (siRNA) after transfection may unravel new ways to improve RNA interference (RNAi) efficiency. Because intracellular degradation of RNA may prevent reliable observation of fluorescence-labeled siRNA, new tools for fluorescence microscopy are warranted to cover the considerable duration of the RNAi effect. Here, the characterization and application of new fluorescence resonance energy transfer (FRET) dye pairs for sensing the integrity of duplex siRNA is reported, which allows an assessment of the degradation status of an siRNA cell population by live cell imaging. A panel of high-yield fluorescent dyes has been investigated for their suitability as FRET pairs for the investigation of RNA inside the cell. Nine dyes in 13 FRET pairs were evaluated based on the performance in assays of photostability, cross-excitation, bleed-through, as well as on quantified changes of fluorescence as a consequence of, e.g., RNA strand hybridization and pH variation. The Atto488/Atto590 FRET pair has been applied to live cell imaging, and has revealed first aspects of unusual trafficking of intact siRNA. A time-lapse study showed highly dynamic movement of siRNA in large perinuclear structures. These and the resulting optimized FRET labeled siRNA are expected to have significant impact on future observations of labeled RNAs in living cells.

Advances in Microscopy Techniques

2011 ◽  
Vol 135 (2) ◽  
pp. 255-263
Author(s):  
Daniel B Schmolze ◽  
Clive Standley ◽  
Kevin E Fogarty ◽  
Andrew H Fischer
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Near Field ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Molecular Techniques ◽  
Live Cell ◽  
Stimulated Emission ◽  
Structured Illumination ◽  
Microscopy Techniques

Abstract Context.—Advances in microscopy enable visualization of a broad range of new morphologic features. Objective.—To review and illustrate advances in microscopy with relevance to pathologists. Data Sources.—Literature review and new observations. Results.—Fluorescence microscopy enables multiantigen detection; allows novel optical-sectioning techniques, with some advantages compared to paraffin sectioning; and permits live-cell imaging. Live-cell imaging allows pathologists to move from a period when all diagnostic expertise was reliant on interpreting static images to a period when cellular dynamics can play a role in diagnosis. New techniques have bypassed by about 100-fold what had long been believed to be a limit to the resolution of light microscopy. Fluorescence resonance energy transfer (FRET) appears capable of visualizing diagnostically relevant molecular events in living or fixed cells that are immeasurable by other molecular techniques. We describe applications of 2-photon microscopy, FRET, structured illumination, and the subdiffraction techniques of near-field microscopy, photoactivated localization microscopy, stochastic optical reconstruction microscopy, and stimulated emission depletion microscopy. Conclusion.—New microscopy techniques present opportunities for pathologists to develop improved diagnostic tests.

scholarly journals Nanoscale optical writing through upconversion resonance energy transfer

2021 ◽  
Vol 7 (9) ◽  
pp. eabe2209
Author(s):  
S. Lamon ◽  
Y. Wu ◽  
Q. Zhang ◽  
X. Liu ◽  
M. Gu
Keyword(s):  
Graphene Oxide ◽  
Energy Transfer ◽  
Energy Consumption ◽  
Data Storage ◽  
High Capacity ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
High Energy ◽  
Optical Data ◽  
Upconversion Nanoparticles

Nanoscale optical writing using far-field super-resolution methods provides an unprecedented approach for high-capacity data storage. However, current nanoscale optical writing methods typically rely on photoinitiation and photoinhibition with high beam intensity, high energy consumption, and short device life span. We demonstrate a simple and broadly applicable method based on resonance energy transfer from lanthanide-doped upconversion nanoparticles to graphene oxide for nanoscale optical writing. The transfer of high-energy quanta from upconversion nanoparticles induces a localized chemical reduction in graphene oxide flakes for optical writing, with a lateral feature size of ~50 nm (1/20th of the wavelength) under an inhibition intensity of 11.25 MW cm−2. Upconversion resonance energy transfer may enable next-generation optical data storage with high capacity and low energy consumption, while offering a powerful tool for energy-efficient nanofabrication of flexible electronic devices.

scholarly journals Contribution of resonance energy transfer to the luminescence quenching of upconversion nanoparticles with graphene oxide

2020 ◽  
Vol 575 ◽  
pp. 119-129 ◽  
Author(s):  
Diego Mendez-Gonzalez ◽  
Oscar G. Calderón ◽  
Sonia Melle ◽  
Jesús González-Izquierdo ◽  
Luis Bañares ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Luminescence Quenching ◽  
Upconversion Nanoparticles
Sign in / Sign up

Export Citation Format

Share Document