A protein–polymer hybrid gene carrier based on thermophilic histone and polyethylenimine

2015 ◽  
Vol 39 (9) ◽  
pp. 6718-6721 ◽  
Author(s):  
Hui Shi ◽  
Haobo Han ◽  
Zhen Xing ◽  
Jiawen Chen ◽  
Yudi Wang ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Carrier ◽  
Hybrid Gene ◽  
Protein Polymer ◽  
High Transfection Efficiency ◽  
Polymer Hybrid ◽  
P Protein ◽  
Low Cytotoxicity

Protein–polymer hybrid gene carrier with high transfection efficiency and low cytotoxicity.

Bio-inspired gene carriers with low cytotoxicity constructed via the assembly of dextran nanogels and nano-coacervates

Nanomedicine ◽  
2020 ◽  
Vol 15 (13) ◽  
pp. 1285-1296 ◽  
Author(s):  
Chenglong Wang ◽  
Jiayi You ◽  
Miaomiao Gao ◽  
Peipei Zhang ◽  
Guoxiong Xu ◽  
...  
Keyword(s):  
Positive Charge ◽  
Transfection Efficiency ◽  
Gene Carrier ◽  
Gene Carriers ◽  
Environmentally Responsive ◽  
Potential Applications ◽  
Internal Peptide ◽  
The Common ◽  
Low Cytotoxicity ◽  
Peptide Gene

Aim: To achieve safe and biocompatible gene carriers. Materials & methods: A core/shell-structured hierarchical carrier with an internal peptide/gene coacervate ‘core’ and a dextran nanogel ‘shell’ on the surface has been designed. Results: The dextran nanogels shield coacervate (DNSC) can effectively condense genes and release them in reducing environments. The dextran nanogel-based ‘shell’ can effectively shield the positive charge of the peptide/gene coacervate ‘core’, thus reducing the side effects of cationic gene carriers. In contrast with the common nonviral gene carriers that had high cytotoxicities, the DNSC showed a high transfection efficiency while maintaining a low cytotoxicity. Conclusion: The DNSC provides an effective environmentally responsive gene carrier with potential applications in the fields of gene therapy and gene carrier development.

Biodegradable nano-organosilica gene carrier for high-efficiency gene transfection

2020 ◽  
Vol 8 (12) ◽  
pp. 2483-2494
Author(s):  
Kun Zeng ◽  
Li Ma ◽  
Wenxiu Yang ◽  
Shan Lei ◽  
Mozhen Wang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Carrier ◽  
Gene Vector ◽  
High Transfection Efficiency ◽  
In Cells

Guanidinated-fluorinated α-polylysine-modified organosilica nanoparticles can form a novel raisin-bread-like gene vector, which is disintegrated in cells by GSH to show high transfection efficiency.

Defined pH-sensitive nanogels as gene delivery platform for siRNA mediated in vitro gene silencing

2017 ◽  
Vol 5 (11) ◽  
pp. 2328-2336 ◽  
Author(s):  
Mathias Dimde ◽  
Falko Neumann ◽  
Felix Reisbeck ◽  
Svenja Ehrmann ◽  
Jose Luis Cuellar-Camacho ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Gene Silencing ◽  
Transfection Efficiency ◽  
Genetic Material ◽  
Ph Sensitive ◽  
Carrier System ◽  
High Transfection Efficiency ◽  
Delivery Platform ◽  
Low Cytotoxicity

An advanced cationic carrier system which combines high transfection efficiency with low cytotoxicity and a control over the release of the encapsulated genetic material by the reduction of the multivalent architecture upon pH triggered degradation was developed.

α,β-Poly(l-aspartate-graft-PEI): A pseudo-branched PEI as a gene carrier with low toxicity and high transfection efficiency

2009 ◽  
Vol 5 (7) ◽  
pp. 2485-2494 ◽  
Author(s):  
Jia-Hui Yu ◽  
Ji-Shan Quan ◽  
Jin Huang ◽  
Cheng-Yun Wang ◽  
Bo Sun ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Carrier ◽  
High Transfection Efficiency ◽  
Low Toxicity

scholarly journals A self-assembling polycationic nanocarrier that exhibits exceptional gene transfection efficiency

RSC Advances ◽  
2015 ◽  
Vol 5 (111) ◽  
pp. 91619-91632 ◽  
Author(s):  
Kishor Sarkar ◽  
Sai Rama Krishna Meka ◽  
Giridhar Madras ◽  
Kaushik Chatterjee
Keyword(s):  
Molecular Weight ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Carrier ◽  
Low Molecular Weight ◽  
High Transfection Efficiency ◽  
Self Assembling

A novel polycationic gene carrier was prepared by conjugation of low molecular weight polyethyleneimine with gelatin through 4-bromonaphthaleic anhydride with exceptionally high transfection efficiency.

Efficient gene transfection to liver cells via the cellular regulation of a multifunctional polylactitol-based gene transporter

2016 ◽  
Vol 4 (12) ◽  
pp. 2208-2218 ◽  
Author(s):  
Young-Dong Kim ◽  
Tae-Eun Park ◽  
Bijay Singh ◽  
Kye-Soo Cho ◽  
Jaiprakash N. Sangshetti ◽  
...  
Keyword(s):  
Liver Cell ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Carrier ◽  
Cell Targeting ◽  
Cellular Regulation ◽  
Efficient Gene ◽  
Low Cytotoxicity

A new polylactitol-based multifunctional gene carrier has shown low cytotoxicity, a high transfection efficiency, and liver cell targeting bothin vitroandin vivo.

scholarly journals Fabrication and Biological Activities of Plasmid DNA Gene Carrier Nanoparticles Based on Biodegradable l-Tyrosine Polyurethane

2021 ◽  
Vol 15 (1) ◽  
pp. 17
Author(s):  
Soo-Yong Park ◽  
Yang H. Yun ◽  
Bum-Joon Park ◽  
Hyung-Il Seo ◽  
Ildoo Chung
Keyword(s):  
Gene Therapy ◽  
Cellular Uptake ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Biological Activities ◽  
Average Diameter ◽  
Gene Carrier ◽  
Suitable Alternative ◽  
High Transfection Efficiency ◽  
Gene Carriers

Gene therapy is a suitable alternative to chemotherapy due to the complications of drug resistance and toxicity of drugs, and is also known to reduce the occurrence of cellular mutation through the use of gene carriers. In this study, gene carrier nanoparticles with minimal toxicity and high transfection efficiency were fabricated from a biocompatible and biodegradable polymer, l-tyrosine polyurethane (LTU), which was polymerized from presynthesized desaminotyrosyl tyrosine hexyl ester (DTH) and polyethylene glycol (PEG), by using double emulsion and solvent evaporation techniques, resulting in the formation of porous nanoparticles, and then used to evaluate their potential biological activities through molecular controlled release and transfection studies. To assess cellular uptake and transfection efficiency, two model drugs, fluorescently labeled bovine serum albumin (FITC-BSA) and plasmid DNA-linear polyethylenimine (LPEI) complex, were successfully encapsulated in nanoparticles, and their transfection properties and cytotoxicities were evaluated in LX2 as a normal cell and in HepG2 and MCF7 as cancer cells. The morphology and average diameter of the LTU nanoparticles were confirmed using light microscopy, transmission electron microscopy, and dynamic light scattering, while confocal microscopy was used to validate the cellular uptake of FITC-BSA-encapsulated LTU nanoparticles. Moreover, the successful cellular uptake of LTU nanoparticles encapsulated with pDNA-LPEI and the high transfection efficiency, confirmed by gel electrophoresis and X-gal assay transfection, indicated that LTU nanoparticles had excellent cell adsorption ability, facilitated gene encapsulation, and showed the sustained release tendency of genes through transfection experiments, with an optimal concentration ratio of pDNA and LPEI of 1:10. All the above characteristics are ideal for gene carriers designed to transport and release drugs into the cytoplasm, thus facilitating effective gene therapy.

Novel Poly(Ester Amine) Based on Polycaprolactone and Polyethylenimine as a Gene Carrier: Effect of Hydrophobicity on Transfection Efficiency and Cytotoxicity

2007 ◽  
Vol 342-343 ◽  
pp. 453-456 ◽  
Author(s):  
Rohidas B. Arote ◽  
Tae Hee Kim ◽  
You Kyoung Kim ◽  
Dhananjay Jere ◽  
Hu Lin Jiang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Carrier ◽  
Particle Sizes ◽  
Dna Complexes ◽  
Novel Gene ◽  
Carrier Effect ◽  
Biodegradable Poly ◽  
Low Cytotoxicity

Novel, biodegradable poly(ester amine)s (PEAs) were synthesized using hydrophobic polycaprolactone diacrylate (PCLDA) and highly cationic polyethylenimine (PEI). This novel gene carrier can form stable DNA complexes with particle sizes around 200 nm, and showing excellent transfection efficiency and relatively low cytotoxicity compared with PEI 25K. Effect of hydrophobicity on transfection efficiency and cytotoxicity was profound and was relatively important parameter for the success of gene delivery.

scholarly journals In Vitro Cellular Uptake and Transfection of Oligoarginine-Conjugated Glycol Chitosan/siRNA Nanoparticles

Polymers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 4219
Author(s):  
Eun-Ju Jeong ◽  
Jangwook Lee ◽  
Hyun-Seung Kim ◽  
Kuen-Yong Lee
Keyword(s):  
Transfection Efficiency ◽  
Physicochemical Characteristics ◽  
Gene Carrier ◽  
Glycol Chitosan ◽  
Arginine Residues ◽  
Low Toxicity ◽  
Low Cytotoxicity ◽  
Low Solubility ◽  
Positively Charged

Chitosan and its derivatives have been extensively utilized in gene delivery applications because of their low toxicity and positively charged characteristics. However, their low solubility under physiological conditions often limits their application. Glycol chitosan (GC) is a derivative of chitosan that exhibits excellent solubility in physiological buffer solutions. However, it lacks the positive characteristics of a gene carrier. Thus, we hypothesized that the introduction of oligoarginine peptide to GC could improve the formation of complexes with siRNA, resulting in enhanced uptake by cells and increased transfection efficiency in vitro. A peptide with nine arginine residues and 10 glycine units (R9G10) was successfully conjugated to GC, which was confirmed by infrared spectroscopy, 1H NMR spectroscopy, and elemental analysis. The physicochemical characteristics of R9G10-GC/siRNA complexes were also investigated. The size and surface charge of the R9G10-GC/siRNA nanoparticles depended on the amount of R9G10 coupled to the GC. In addition, the R9G10-GC/siRNA nanoparticles showed improved uptake in HeLa cells and enhanced in vitro transfection efficiency while maintaining low cytotoxicity determined by the MTT assay. Oligoarginine-modified glycol chitosan may be useful as a potential gene carrier in many therapeutic applications.

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