Characterization of zinc uptake by mouse primary cultured astrocytes and microglia

Metallomics ◽  
2015 ◽  
Vol 7 (7) ◽  
pp. 1067-1077 ◽  
Author(s):  
Shohei Segawa ◽  
Nao Tatsumi ◽  
Akihiro Ohishi ◽  
Kentaro Nishida ◽  
Kazuki Nagasawa
Keyword(s):  
Zinc Uptake ◽  
Cultured Astrocytes ◽  
Primary Cultured Astrocytes

Potassium currents in primary cultured astrocytes from the rat corpus callosum

2005 ◽  
Vol 34 (6) ◽  
pp. 411-420 ◽  
Author(s):  
Daniel Reyes-Haro ◽  
Ricardo Miledi ◽  
Jesús García-Colunga
Keyword(s):  
Corpus Callosum ◽  
Potassium Currents ◽  
Cultured Astrocytes ◽  
Primary Cultured Astrocytes

Role of Na/K/Cl cotransport in astrocytes

1992 ◽  
Vol 70 (S1) ◽  
pp. S260-S262 ◽  
Author(s):  
Wolfgang Walz
Keyword(s):  
Volume Regulation ◽  
Ion Homeostasis ◽  
Combined Cycle ◽  
Kinetic Characterization ◽  
Cultured Astrocytes ◽  
Net Uptake ◽  
Swelling Volume ◽  
External K

The kinetic characterization of the Na/K/Cl cotransport of cultured astrocytes and evidence for its involvement in volume regulation and K+ net uptake during K+ clearance are reviewed. Emphasis is put on experimental evidence for a proposed sodium cycle in astrocytes; this cycle involves a Na+–K+ ATPase that is stimulated by both a high external K+ and intracellular Na+. Elevated external K+ also stimulates the Na/K/Cl carrier, transporting these ions inward. As a result Na+ is cycled across the membrane, carried inward by the Na/K/Cl carrier, and returned by the Na+–K+ ATPase. Both functionally coupled mechanisms lead to intracellular KCl accumulation and inward movements of water to compensate for increased osmolarity. The combined cycle is expected to play a major role in the regulation of physiological K+ levels in the brain.Key words: furosemide, ion homeostasis, Na+–K+ ATPase, swelling, volume regulation.

scholarly journals Zinc uptake in the Basidiomycota: Characterization of zinc transporters in Ustilago maydis

2019 ◽  
Vol 35 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Adriana M. Martha-Paz ◽  
David Eide ◽  
David Mendoza-Cózatl ◽  
Norma A. Castro-Guerrero ◽  
Elva T. Aréchiga-Carvajal
Keyword(s):  
Ustilago Maydis ◽  
Zinc Uptake ◽  
Zinc Transporters

scholarly journals Characterization of a Mycobacterium tuberculosis ESX-3 Conditional Mutant: Essentiality and Rescue by Iron and Zinc

2009 ◽  
Vol 191 (20) ◽  
pp. 6340-6344 ◽  
Author(s):  
Agnese Serafini ◽  
Francesca Boldrin ◽  
Giorgio Palù ◽  
Riccardo Manganelli
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Secretory Pathway ◽  
Iron Homeostasis ◽  
Zinc Uptake ◽  
Wild Type ◽  
Secretion Systems ◽  
Type Vii Secretion ◽  
Conditional Mutant ◽  
Iron And Zinc

ABSTRACT Recently, a novel type of secretory pathway, type VII secretion systems (T7SSs), has been characterized in mycobacteria. The chromosomes of Mycobacterium tuberculosis and Mycobacterium bovis encode five T7SSs (ESX-1 to ESX-5). The best characterized of them, ESX-1, is involved in host-pathogen interactions, and its deletion is one of the main causes of M. bovis BCG attenuation. Another T7SS, ESX-3, has been previously shown to be transcriptionally controlled by the zinc uptake repressor (Zur) and by the iron-dependent transcriptional repressor (IdeR), suggesting that it might be involved in zinc and iron homeostasis. In this study, we characterized an M. tuberculosis conditional mutant in which transcription of the ESX-3 gene cluster can be downregulated by anhydrotetracycline. We showed that this T7SS is essential for growth and that this phenotype can be complemented by zinc, iron, or supernatant from a wild-type parental strain culture, demonstrating that the ESX-3 secretion system is responsible for the secretion of some soluble factor(s) required for growth that is probably involved in optimal iron and zinc uptake.

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