Highly efficient integration of the viral portal proteins from different types of phages into planar bilayers for the black lipid membrane analysis

2016 ◽  
Vol 12 (2) ◽  
pp. 480-489 ◽  
Author(s):  
Peng Jing ◽  
Hallel Paraiso ◽  
Benjamin Burris
Keyword(s):  
Lipid Membrane ◽  
Planar Bilayers ◽  
Black Lipid Membrane ◽  
Highly Efficient ◽  
Different Types ◽  
Efficient Integration

An effective method used to prepare fusible proteoliposomes reconstituted with phage portal proteins for the black lipid membrane analysis.

scholarly journals Highly efficient reusable superhydrophobic sponge prepared by a facile, simple and cost effective biomimetic bonding method for oil absorption

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiaqi Wang ◽  
Yan Chen ◽  
Qinyao Xu ◽  
Miaomiao Cai ◽  
Qian Shi ◽  
...  
Keyword(s):  
Environmentally Friendly ◽  
Absorption Capacity ◽  
Oil Absorption ◽  
Water Contact ◽  
Water Separation ◽  
Highly Efficient ◽  
Study Results ◽  
Different Types ◽  
Modification Method ◽  
Bonding Method

AbstractSuperhydrophobic sponges have considerable potential for oil/water separation. Most of the methods used for superhydrophobic modification of sponges require toxic or harmful solvents, which have the drawbacks of hazardous to environment, expensive, and complex to utilize. Moreover, the hydrophobic layer on the surface of sponge is often easily destroyed. In this paper, a highly efficient superhydrophobic sponge with excellent reusability was developed by using a facile, simple and environmentally friendly dopamine biomimetic bonding method. Different types of sponges, such as melamine, polyethylene or polyurethane sponge wastes, were used as raw materials to prepare superhydrophobic sponges, which possess the advantages of inexpensive and abundant. The effects of different dopamine polymerization time and different hydrophobic agent dosage on the hydrophobicity and oil absorption capacity of melamine sponges were optimized. The study results showed that the water contact angle of the superhydrophobic sponge could reach 153° with excellent organic solvent absorption capacity of 165.9 g/g. Furthermore, the superhydrophobic sponge retained approximately 92.1% of its initial absorption capacity after 35 reutilization cycles. More importantly, the dopamine biomimetic bonding superhydrophobic modification method can be used for different types of sponges. Therefore, a universally applicable, facile, simple and environmentally friendly superhydrophobic modification method for sponges was developed.

scholarly journals The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

2019 ◽  
Vol 20 (20) ◽  
pp. 5217
Author(s):  
Hans-Joachim Freisleben
Keyword(s):  
Lipid Membrane ◽  
Micrococcus Luteus ◽  
Atp Synthesis ◽  
Scanning Calorimetry ◽  
Thermoplasma Acidophilum ◽  
Black Lipid Membrane ◽  
Langmuir Blodgett ◽  
Hydrophobic Peptides ◽  
Laboratory Results ◽  
Review Reports

The main phospholipid (MPL) of Thermoplasma acidophilum DSM 1728 was isolated, purified and physico-chemically characterized by differential scanning calorimetry (DSC)/differential thermal analysis (DTA) for its thermotropic behavior, alone and in mixtures with other lipids, cholesterol, hydrophobic peptides and pore-forming ionophores. Model membranes from MPL were investigated; black lipid membrane, Langmuir-Blodgett monolayer, and liposomes. Laboratory results were compared to computer simulation. MPL forms stable and resistant liposomes with highly proton-impermeable membrane and mixes at certain degree with common bilayer-forming lipids. Monomeric bacteriorhodopsin and ATP synthase from Micrococcus luteus were co-reconstituted and light-driven ATP synthesis measured. This review reports about almost four decades of research on Thermoplasma membrane and its MPL as well as transfer of this research to Thermoplasma species recently isolated from Indonesian volcanoes.

scholarly journals Membrane phospholipid composition affects function of potassium channels from rabbit colon epithelium

1999 ◽  
Vol 277 (1) ◽  
pp. C83-C90 ◽  
Author(s):  
Klaus Turnheim ◽  
Johannes Gruber ◽  
Christoph Wachter ◽  
Valentina Ruiz-Gutiérrez
Keyword(s):  
Surface Potential ◽  
Lipid Membrane ◽  
Single Channel ◽  
Membrane Surface ◽  
Phospholipid Composition ◽  
Distal Colon ◽  
Membrane Phospholipids ◽  
Channel Conductance ◽  
Planar Bilayers ◽  
Conduction Pathway

We tested the effects of membrane phospholipids on the function of high-conductance, Ca2+-activated K+ channels from the basolateral cell membrane of rabbit distal colon epithelium by reconstituting these channels into planar bilayers consisting of different 1:1 mixtures of phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS), and phosphatidylinositol (PI). At low ambient K+ concentrations single-channel conductance is higher in PE/PS and PE/PI bilayers than in PE/PC bilayers. At high K+concentrations this difference in channel conductance is abolished. Introducing the negatively charged SDS into PE/PC bilayers increases channel conductance, whereas the positively charged dodecyltrimethylammonium has the opposite effect. All these findings are consistent with modulation of channel current by the charge of the lipid membrane surrounding the channel. But the K+ that permeates the channel senses only a small fraction of the full membrane surface potential of the charged phospholipid bilayers, equivalent to separation of the conduction pathway from the charged phospholipid head groups by 20Å. This distance appears to insulate the channel entrance from the bilayer surface potential, suggesting large dimensions of the channel-forming protein. In addition, in PE/PC and PE/PI bilayers, but not in PE/PS bilayers, the open-state probability of the channel decreases with time (“channel rundown”), indicating that phospholipid properties other than surface charge are required to maintain channel fluctuations.

scholarly journals Highly efficient integration and expression of piggyBac-derived cassettes in the honeybee (Apis mellifera)

2014 ◽  
Vol 111 (24) ◽  
pp. 9003-9008 ◽  
Author(s):  
C. Schulte ◽  
E. Theilenberg ◽  
M. Muller-Borg ◽  
T. Gempe ◽  
M. Beye
Keyword(s):  
Apis Mellifera ◽  
Highly Efficient ◽  
Efficient Integration

scholarly journals Extracellular vesicles in the host-helminth communication: biomedical applications

2021 ◽  
Vol 87 (87(03)) ◽  
pp. 351-360
Author(s):  
Antonio Marcilla Díaz
Keyword(s):  
Extracellular Vesicles ◽  
Biomedical Applications ◽  
Lipid Membrane ◽  
Biological Fluids ◽  
Different Types ◽  
Health And Disease ◽  
Secretion Of Mediators ◽  
New Treatments ◽  
Intercellular Communications ◽  
Biomedical Interest

Extracellular vesicles participate in intercellular communications, altogether with classic mechanisms like direct contact between cells and the secretion of mediators. They have attracted considerable interest since their discovery in reticulocytes in 1983. The term includes different types of vesicles that vary in size and origin, with exosomes, microvesicles and apoptotic bodies as the major ones. These structures are sorrounded by a lipid membrane, where various types of receptors are located, and can carry different cargo molecules, including sugars, proteins, nucleic acids and metabolites. They have been described in all kingdoms in nature (participating in both intercellular and inter-specific communications), in all types of biological fluids (as part of liquid biopsy). In fact, their presence in samples from both physiological and pathological processes has suggested them as excellent biomarkers. Their role in health and disease is being widely investigated. In this context, the study of extracellular vesicles produced by parasites, and specifically by helminths, constitutes a growing field of research, with great biomedical interest, mainly in the control of infections caused by them. In fact, these vesicles can be used to generate rapid and specific diagnosis systems, to produce new tools for vaccination, and to identify targets for new treatments. The ability of extracellular vesicles to modulate the immune response also opens new possibilities for their use against autoimmune diseases.

scholarly journals Highly Efficient Prodrugs: Design and Therapeutic Applications

2020 ◽  
Vol 36 (6) ◽  
pp. 985-1000
Author(s):  
Ashutosh Pal ◽  
Bimal Krishna Banik
Keyword(s):  
Drug Administration ◽  
Active Compound ◽  
Active Drug ◽  
Target Delivery ◽  
Specific Target ◽  
Therapeutic Applications ◽  
Highly Efficient ◽  
Different Types

Prodrug is a very powerful way for the improvement of biopharmaceutical, physicochemical, or pharmacokinetic possessions of pharmacologically dynamic mediators. Prodrug is a pharmacologically not an active compound, which can be converted into an active drug by biotransformation which is metabolic and such process the efficiency of drugs gets improved with specific target delivery. The conversion of a prodrug to drug may happen before concentration, after concentration, or at a precise part of the physique. This approach has many advantages over drug administration which is in our convention. In this review, different types of carriers, which can be used for prodrug synthesis are summarized. Examples of both marketed and investigational prodrugs from several promoieties are discussed not only for their advantages and uses but also their prospects. The purpose of this review is to introduce in detail the foundation behind the use of the prodrug methodology from past to present, and at the same time, to consider the possible consequences, which may evolve from insufficient initiation of prodrugs. Furthermore, the concept of prodrug and the classifications of prodrugs will be discussed in this article and it is expected that this review will be helpful for medicinal chemists for their research in the upcoming days.

scholarly journals Lipid II-Mediated Pore Formation by the Peptide Antibiotic Nisin: a Black Lipid Membrane Study

2004 ◽  
Vol 186 (10) ◽  
pp. 3259-3261 ◽  
Author(s):  
Imke Wiedemann ◽  
Roland Benz ◽  
Hans-Georg Sahl
Keyword(s):  
Cell Wall ◽  
Pore Formation ◽  
Cytoplasmic Membrane ◽  
Lipid Membrane ◽  
Specific Interaction ◽  
Peptide Antibiotic ◽  
Black Lipid Membrane ◽  
Lipid Ii ◽  
Antibiotic Peptide

ABSTRACT The antibiotic peptide nisin is the first known lantibiotic that uses a docking molecule within the bacterial cytoplasmic membrane for pore formation. Through specific interaction with the cell wall precursor lipid II, nisin forms defined pores which are stable for seconds and have pore diameters of 2 to 2.5 nm.

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