Characterization of host response to Cryptococcus neoformans through quantitative proteomic analysis of cryptococcal meningitis co-infected with HIV

2015 ◽  
Vol 11 (9) ◽  
pp. 2529-2540 ◽  
Author(s):  
Lakshmi Dhevi N. Selvan ◽  
Sreelakshmi K. Sreenivasamurthy ◽  
Satwant Kumar ◽  
Soujanya D. Yelamanchi ◽  
Anil K. Madugundu ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Cryptococcal Meningitis ◽  
Proteomic Analysis ◽  
Host Response ◽  
Quantitative Proteomic Analysis ◽  
Brain Proteome ◽  
Proteome Profile

Brain proteome profile of cryptococcal meningitis co-infected with HIV.

Identification of Host-Response in Cerebral Malaria Patients Using Quantitative Proteomic Analysis

2018 ◽  
Vol 12 (4) ◽  
pp. 1600187 ◽  
Author(s):  
Manish Kumar ◽  
Chakrakodi N. Varun ◽  
Gourav Dey ◽  
Raju Ravikumar ◽  
Anita Mahadevan ◽  
...  
Keyword(s):  
Cerebral Malaria ◽  
Proteomic Analysis ◽  
Host Response ◽  
Quantitative Proteomic Analysis

scholarly journals Quantitative proteomic analysis and functional characterization of Acanthamoeba castellanii exosome-like vesicles

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei-Chen Lin ◽  
Chia-Yun Tsai ◽  
Jian-Ming Huang ◽  
Shang-Rung Wu ◽  
Lichieh Julie Chu ◽  
...  
Keyword(s):  
Immune Response ◽  
Proteomic Analysis ◽  
Functional Characterization ◽  
Acanthamoeba Castellanii ◽  
Host Cells ◽  
C6 Cells ◽  
Proteomic Profiling ◽  
Quantitative Proteomic Analysis ◽  
Rat Glioma

Abstract Background Pathogenic protozoans use extracellular vesicles (EVs) for intercellular communication and host manipulation. Acanthamoeba castellanii is a free-living protozoan that may cause severe keratitis and fatal granulomatous encephalitis. Although several secreted molecules have been shown to play crucial roles in the pathogenesis of Acanthamoeba, the functions and components of parasite-derived EVs are far from understood. Methods Purified EVs from A. castellanii were confirmed by electron microscopy and nanoparticle tracking analysis. The functional roles of parasite-derived EVs in the cytotoxicity to and immune response of host cells were examined. The protein composition in EVs from A. castellanii was identified and quantified by LC-MS/MS analysis. Results EVs from A. castellanii fused with rat glioma C6 cells. The parasite-derived EVs induced an immune response from human THP-1 cells and a cytotoxic effect in C6 cells. Quantitative proteomic analysis identified a total of 130 proteins in EVs. Among the identified proteins, hydrolases (50.2%) and oxidoreductases (31.7%) were the largest protein families in EVs. Furthermore, aminopeptidase activities were confirmed in EVs from A. castellanii. Conclusions The proteomic profiling and functional characterization of EVs from A. castellanii provide an in-depth understanding of the molecules packaged into EVs and their potential mechanisms mediating the pathogenesis of this parasite.

scholarly journals Characterization of the Mouse Brain Proteome Using Global Proteomic Analysis Complemented with Cysteinyl-Peptide Enrichment

2006 ◽  
Vol 5 (2) ◽  
pp. 361-369 ◽  
Author(s):  
Haixing Wang ◽  
Wei-Jun Qian ◽  
Mark H. Chin ◽  
Vladislav A. Petyuk ◽  
Richard C. Barry ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Proteomic Analysis ◽  
Brain Proteome ◽  
Peptide Enrichment
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