Plasma metabolic profiling after cortical spreading depression in a transgenic mouse model of hemiplegic migraine by capillary electrophoresis – mass spectrometry

2015 ◽  
Vol 11 (5) ◽  
pp. 1462-1471 ◽  
Author(s):  
Reinald Shyti ◽  
Isabelle Kohler ◽  
Bart Schoenmaker ◽  
Rico J. E. Derks ◽  
Michel D. Ferrari ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Metabolic Profiling ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Transgenic Mouse Model ◽  
Metabolic Changes ◽  
Hemiplegic Migraine

Cortical spreading depression-induced brain metabolic changes have been captured in the plasma of a transgenic migraine mouse model using CE-MS.

scholarly journals Cortical Spreading Depression Causes Unique Dysregulation of Inflammatory Pathways in a Transgenic Mouse Model of Migraine

2016 ◽  
Vol 54 (4) ◽  
pp. 2986-2996 ◽  
Author(s):  
Else Eising ◽  
Reinald Shyti ◽  
Peter A. C. ’t Hoen ◽  
Lisanne S. Vijfhuizen ◽  
Sjoerd M. H. Huisman ◽  
...  
Keyword(s):  
Mouse Model ◽  
Transgenic Mouse ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Transgenic Mouse Model ◽  
Inflammatory Pathways

scholarly journals NMR-based metabolic profiling of urine, serum, fecal, and pancreatic tissue samples from the Ptf1a-Cre; LSL-KrasG12D transgenic mouse model of pancreatic cancer

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0200658 ◽  
Author(s):  
Michelle J. Schmahl ◽  
Daniel P. Regan ◽  
Adam C. Rivers ◽  
William C. Joesten ◽  
Michael A. Kennedy
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Metabolic Profiling ◽  
Transgenic Mouse Model ◽  
Pancreatic Tissue ◽  
Tissue Samples ◽  
Urine Serum

scholarly journals Brain insulin response and peripheral metabolic changes in a Tau transgenic mouse model

2019 ◽  
Vol 125 ◽  
pp. 14-22 ◽  
Author(s):  
Antoine Leboucher ◽  
Tariq Ahmed ◽  
Emilie Caron ◽  
Anne Tailleux ◽  
Sylvie Raison ◽  
...  
Keyword(s):  
Mouse Model ◽  
Transgenic Mouse ◽  
Insulin Response ◽  
Transgenic Mouse Model ◽  
Metabolic Changes ◽  
Brain Insulin

scholarly journals Characteristics of cortical spreading depression and c-Fos expression in transgenic mice having a mutation associated with familial hemiplegic migraine 2

Cephalalgia ◽  
2020 ◽  
Vol 40 (11) ◽  
pp. 1177-1190
Author(s):  
Chunhua Tang ◽  
Miyuki Unekawa ◽  
Mamoru Shibata ◽  
Yutaka Tomita ◽  
Yoshikane Izawa ◽  
...  
Keyword(s):  
Mouse Model ◽  
Transgenic Mice ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Orthologous Gene ◽  
Underlying Mechanism ◽  
Familial Hemiplegic Migraine ◽  
Wild Type ◽  
Hemiplegic Migraine ◽  
Fos Expression

Background Cortical spreading depression is thought to be the underlying mechanism of migraine aura. In 2006, three relatives having the point mutation E700K in ATP1A2 exon 15 were diagnosed with familial hemiplegic migraine 2 characterized by complicated forms of aura. Here, we generated a transgenic mouse model having the human E700K mutation in the Atp1a2 orthologous gene. Objective To investigate the characteristics of cortical spreading depression in a mouse model with E700K mutation in the Atp1a2. Methods Cortical spreading depression was induced by applying stepwise increases of KCl concentration or electrical stimulation intensity to C57BL/6J-Tg(Atp1a2*E700K)9151Kwk mice (Tg, both sexes) and corresponding wild-type animals. Under urethane anesthesia, the responsiveness and threshold to cortical spreading depression were examined and the distribution of c-Fos expression, a neuronal activity marker, was immunohistochemically determined. Results Overall, Tg mice showed significantly faster propagation velocity ( p < 0.01) and longer full-width-at-half-maximum ( p < 0.01) than wild-type animals, representing a slower recovery from direct current potential deflection. The cortical spreading depression threshold tended to be lower in Tg, especially in females. c-Fos-positive cells were significantly enhanced in the ipsilateral somatosensory cortex, piriform cortex, amygdala and striatum (each p < 0.05 vs. contralateral side). Numbers of c-Fos positive cells were significantly higher in the ipsilateral amygdala of Tg, as compared with wild-type animals ( p < 0.01). Conclusion The effect of cortical spreading depression may be greater in E700K transgenic mice than that in wild-type animals, while the threshold for cortical spreading depression shows little change. Higher c-Fos expression in the amygdala may indicate alterations of the limbic system in Tg, suggesting an enhanced linkage between cortical spreading depression and amygdala connectivity in familial hemiplegic migraine 2 patients.

scholarly journals Increased Susceptibility to Cortical Spreading Depression in the Mouse Model of Familial Hemiplegic Migraine Type 2

PLoS Genetics ◽  
2011 ◽  
Vol 7 (6) ◽  
pp. e1002129 ◽  
Author(s):  
Loredana Leo ◽  
Lisa Gherardini ◽  
Virginia Barone ◽  
Maurizio De Fusco ◽  
Daniela Pietrobon ◽  
...  
Keyword(s):  
Mouse Model ◽  
Cortical Spreading Depression ◽  
Spreading Depression ◽  
Familial Hemiplegic Migraine ◽  
Hemiplegic Migraine ◽  
Increased Susceptibility
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