scholarly journals Correction: An integrated slidable and valveless microdevice with solid phase extraction, polymerase chain reaction, and immunochromatographic strip parts for multiplex colorimetric pathogen detection

Lab on a Chip ◽  
2015 ◽  
Vol 15 (23) ◽  
pp. 4488-4488
Author(s):  
Yong Tae Kim ◽  
Dohwan Lee ◽  
Hyun Young Heo ◽  
Do Hyun Kim ◽  
Tae Seok Seo
Keyword(s):  
Polymerase Chain Reaction ◽  
Solid Phase Extraction ◽  
Pathogen Detection ◽  
Solid Phase ◽  
Phase Extraction ◽  
Chain Reaction ◽  
Immunochromatographic Strip ◽  
Polymerase Chain

An integrated slidable and valveless microdevice with solid phase extraction, polymerase chain reaction, and immunochromatographic strip parts for multiplex colorimetric pathogen detection

Lab on a Chip ◽  
2015 ◽  
Vol 15 (21) ◽  
pp. 4148-4155 ◽  
Author(s):  
Yong Tae Kim ◽  
Dohwan Lee ◽  
Hyun Young Heo ◽  
Do Hyun Kim ◽  
Tae Seok Seo
Keyword(s):  
Polymerase Chain Reaction ◽  
Solid Phase Extraction ◽  
Pathogen Detection ◽  
Solid Phase ◽  
Phase Extraction ◽  
Pathogen Identification ◽  
Chain Reaction ◽  
Immunochromatographic Strip ◽  
Polymerase Chain ◽  
Genetic Analyzer

We developed an integrated slidable microdevice which can perform solid phase extraction, μPCR, immunochromatographic strip detection for multiplex pathogen identification on a portable genetic analyzer.

A valveless microfluidic device for integrated solid phase extraction and polymerase chain reaction for short tandem repeat (STR) analysis

The Analyst ◽  
2011 ◽  
Vol 136 (9) ◽  
pp. 1928 ◽  
Author(s):  
Kristin A. Hagan ◽  
Carmen R. Reedy ◽  
Joan M. Bienvenue ◽  
Alison H. Dewald ◽  
James P. Landers
Keyword(s):  
Polymerase Chain Reaction ◽  
Solid Phase Extraction ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Solid Phase ◽  
Phase Extraction ◽  
Chain Reaction ◽  
Str Analysis ◽  
Polymerase Chain ◽  
Short Tandem

scholarly journals Evidence for wheat, rye, and barley presence in gluten free foods by PCR method – comparison with ELISA method

2011 ◽  
Vol 29 (No. 1) ◽  
pp. 45-50 ◽  
Author(s):  
E. Mašková ◽  
I. Paulíčková ◽  
J. Rysová ◽  
D. Gabrovská
Keyword(s):  
Polymerase Chain Reaction ◽  
Solid Phase ◽  
Phase Extraction ◽  
Gluten Free ◽  
Chloroplast Gene ◽  
Elisa Method ◽  
Chain Reaction ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Selection Of

A method of the evidence for the presence of wheat, rye, and barley in gluten free foods, based on the polymerase chain reaction (PCR), was validated. DNA was isolated from foods by chaotropic solid phase extraction. The PCR method applied was focused on the intron of the chloroplast gene trnL and utilised primers WBR11 and WBR13. Electrophoresed wheat and rye DNAs were characterised by a 201 bp fragment, barley DNA by a 196 bp fragment. The validated PCR method was applied to the selection of 18 gluten free foods, previously found by ELISA method to contain 1 mg or more of gliadin per 100 g food. The presence of wheat was confirmed by PCR method in all foods analysed. The comparison with the results obtained by ELISA method reliably verified the detection limit of PCR method, i.e., 0.02% wheat.

scholarly journals METODE EKSTRAKSI DNA UNTUK DETEKSI MOLEKULER

2017 ◽  
Vol 8 (2) ◽  
pp. 106
Author(s):  
Rosy Hutami ◽  
N Idzni ◽  
R Ranasasmita ◽  
Mira Suprayatmi
Keyword(s):  
Polymerase Chain Reaction ◽  
Liquid Phase ◽  
Dna Extraction ◽  
Processing Time ◽  
Solid Phase ◽  
Extraction Methods ◽  
Phase Extraction ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Dna Extraction Methods

Dalam teknik deteksi molekuler seperti Loop-Amplification Mediated Polymorphism (LAMP) dan Polymerase Chain Reaction (PCR), pembuatan hulu asam deoksiribonukleat (DNA) sangat penting. Ekstraksi fase cair dan ekstraksi fasa padat merupakan beberapa metode ekstraksi DNA yang tersedia. Tujuan dari penelitian ini adalah untuk mengkarakterisasi metode dan produk ekstraksi DNA berdasarkan kemurnian DNA, visualisasi DNA, konsentrasi DNA, dan waktu pemrosesan metode ekstraksi DNA. Metode ekstraksi yang dievaluasi meliputi metode fenol-kloroform (Metode A) sebagai ekstraksi fasa cair dan metode Ekstraksi Surefood kit (Metode B) sebagai ekstraksi fasa padat. Hasil penelitian menunjukkan bahwa Metode A dapat dilakukan pada sampel dengan konsentrasi DNA sangat rendah berkisar antara 7,00 sampai 9,45 ng / μl dengan kemurnian yang baik (1,80-2,10). Meski tidak menunjukkan DNA isolat band pada gel agarosa 1% dan membutuhkan waktu pemrosesan ± 30 jam. Metode B memiliki performa yang baik dalam mengekstraksi sampel dengan DNA dengan konsentrasi tinggi (49,67 sampai 357,28 ng / μl) dengan kemurnian yang baik (1,93 sampai 2,07). Metode ini menunjukkan band untuk setiap sampel DNA pada gel agarose 1% dan membutuhkan waktu ±1 jam. Kedua metode tersebut dapat digunakan untuk preparasi sampel dalam analisis molekuler termasuk tujuan otentikasi halal.KATA KUNCI: fenol, kloroform, Surefood kit, LAMP  DNA EXTRACTION METHOD FOR MOLECULAR DETECTIONABSTRACTIn molecular detection technique such as Loop-Amplification Mediated Polymorphism (LAMP) and Polymerase Chain Reaction (PCR), right upstream preparation of deoxyribonucleic acid (DNA) is very important. Liquid phase extraction and solid phase extraction are some of DNA extraction methods those are available. The purpose of this research was to characterizedthe method and product of DNA extraction based on DNA purity, DNA visualization, DNA concentration, and processing time of DNA extraction methods. Extraction methods evaluated included phenol-chloroform method (Method A)as liquid phase extraction and Surefood Extraction kit method (Method B) as solid phase extraction. Result showed that Method A could be performed on samples with very low DNA concentrations ranging from 7.00 to 9.45 ng/µl with a good purity (1.80 to 2.10). Although,  it showed no DNA isolates bands on gel agarose 1% and need ± 30 hours processing time. Method B had a good performa in extracting sample with high concentration DNA (49.67 to 357.28 ng/µl) with a good purity (1.93 to 2.07). This method showed bands for each DNA samples on gel agarose 1% and need about ± 1 hour processing time. Both methods can be used for sample preparation in molecular analysis including halal authentication purposes.  

scholarly journals Impact of Multiplex Polymerase Chain Reaction Testing for Respiratory Pathogen Detection in Pediatric Patients

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S503-S503
Author(s):  
Courtney C Sutton ◽  
Patti J Walton ◽  
Montgomery F Williams ◽  
Tracey L Bastian ◽  
Michael Wright ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Pathogen Detection ◽  
Pediatric Patients ◽  
Respiratory Pathogen ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Polymerase Chain
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