Microfluidic cell-phoresis enabling high-throughput analysis of red blood cell deformability and biophysical screening of antimalarial drugs

Lab on a Chip ◽  
2015 ◽  
Vol 15 (23) ◽  
pp. 4451-4460 ◽  
Author(s):  
Aline T. Santoso ◽  
Xiaoyan Deng ◽  
Jeong-Hyun Lee ◽  
Kerryn Matthews ◽  
Simon P. Duffy ◽  
...  
Keyword(s):  
Blood Cell ◽  
High Throughput ◽  
Red Blood Cell ◽  
Gel Electrophoresis ◽  
Antimalarial Drugs ◽  
Cell Deformability ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Red Blood Cell Deformability

Extending gel electrophoresis to cells enables high-throughput analysis of red blood cell deformability and biophysical screening for antimalarial drugs.

High-throughput microsphiltration to assess red blood cell deformability and screen for malaria transmission–blocking drugs

2018 ◽  
Vol 13 (6) ◽  
pp. 1362-1376 ◽  
Author(s):  
Julien Duez ◽  
Mario Carucci ◽  
Irene Garcia-Barbazan ◽  
Matias Corral ◽  
Oscar Perez ◽  
...  
Keyword(s):  
Blood Cell ◽  
Malaria Transmission ◽  
High Throughput ◽  
Red Blood Cell ◽  
Cell Deformability ◽  
Transmission Blocking ◽  
Red Blood Cell Deformability

scholarly journals Multiplexed fluidic plunger mechanism for the measurement of red blood cell deformability

Lab on a Chip ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 159-167 ◽  
Author(s):  
Marie-Eve Myrand-Lapierre ◽  
Xiaoyan Deng ◽  
Richard R. Ang ◽  
Kerryn Matthews ◽  
Aline T. Santoso ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Deformability ◽  
Heterogeneous Sample ◽  
Red Blood Cell Deformability

Mechanism for multiplexed measurement of single red blood cell deformability to evaluate pathological cells in a heterogeneous sample.

Limited effects of activated protein C on red blood cell deformability

2013 ◽  
Vol 53 (4) ◽  
pp. 387-391 ◽  
Author(s):  
Michael Piagnerelli ◽  
Hassane Njimi ◽  
Tatiana Veloso Coelho ◽  
Giulia Reggiori ◽  
Diego Castanares Zapatero ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Protein C ◽  
Activated Protein C ◽  
Cell Deformability ◽  
Red Blood Cell Deformability

Susceptibility of density-fractionated erythrocytes to subhaemolytic mechanical shear stress

2018 ◽  
Vol 42 (3) ◽  
pp. 151-157 ◽  
Author(s):  
Antony P McNamee ◽  
Kieran Richardson ◽  
Jarod Horobin ◽  
Lennart Kuck ◽  
Michael J Simmonds
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Shear Stresses ◽  
Mechanical Stimuli ◽  
Cell Deformability ◽  
Red Blood Cell Deformability ◽  
Cell Age ◽  
Low Shear

Introduction: Accumulating evidence demonstrates that subhaemolytic mechanical stresses, typical of circulatory support, induce physical and biochemical changes to red blood cells. It remains unclear, however, whether cell age affects susceptibility to these mechanical forces. This study thus examined the sensitivity of density-fractionated red blood cells to sublethal mechanical stresses. Methods: Red blood cells were isolated and washed twice, with the least and most dense fractions being obtained following centrifugation (1500 g × 5 min). Red blood cell deformability was determined across an osmotic gradient and a range of shear stresses (0.3–50 Pa). Cell deformability was also quantified before and after 300 s exposure to shear stresses known to decrease (64 Pa) or increase (10 Pa) red blood cell deformability. The time course of accumulated sublethal damage that occurred during exposure to 64 Pa was also examined. Results: Dense red blood cells exhibited decreased capacity to deform when compared with less dense cells. Cellular response to mechanical stimuli was similar in trend for all red blood cells, independent of density; however, the magnitude of impairment in cell deformability was exacerbated in dense cells. Moreover, the rate of impairment in cellular deformability, induced by 64 Pa, was more rapid for dense cells. Relative improvement in red blood cell deformability, due to low-shear conditioning (10 Pa), was consistent for both cell populations. Conclusion: Red blood cell populations respond differently to mechanical stimuli: older (more dense) cells are highly susceptible to sublethal mechanical trauma, while cell age (density) does not appear to alter the magnitude of improved cell deformability following low-shear conditioning.

Effects of erythropoietin and aminoguanidine on red blood cell deformability in diabetic azotemic and uremic patients

2001 ◽  
Vol 38 (6) ◽  
pp. 1414-1420 ◽  
Author(s):  
Clinton D. Brown ◽  
Zhong H. Zhao ◽  
Lorraine L. Thomas ◽  
Robert deGroof ◽  
Eli A. Friedman
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Deformability ◽  
Red Blood Cell Deformability ◽  
Uremic Patients

P0705 THE EFFECT OF HIV INFECTION ON RED BLOOD CELL DEFORMABILITY

2009 ◽  
Vol 20 ◽  
pp. S230
Author(s):  
Georgios Athanasiou ◽  
Athanasia Mouzouri ◽  
Dimitra Dimitropoulou ◽  
Leonidia Leonidou ◽  
Harris Bassaris ◽  
...  
Keyword(s):  
Blood Cell ◽  
Hiv Infection ◽  
Red Blood Cell ◽  
Cell Deformability ◽  
Red Blood Cell Deformability

scholarly journals Anti-erythrocyte antibodies may contribute to anaemia in Plasmodium vivax malaria by decreasing red blood cell deformability and increasing erythrophagocytosis

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Luiza Carvalho Mourão ◽  
Paula Magda da Silva Roma ◽  
Jamila da Silva Sultane Aboobacar ◽  
Camila Maia Pantuzzo Medeiros ◽  
Zélia Barbosa de Almeida ◽  
...  
Keyword(s):  
Blood Cell ◽  
Plasmodium Vivax ◽  
Red Blood Cell ◽  
Vivax Malaria ◽  
Cell Deformability ◽  
Red Blood Cell Deformability ◽  
Plasmodium Vivax Malaria
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