scholarly journals On-chip automation of cell-free protein synthesis: new opportunities due to a novel reaction mode

Lab on a Chip ◽  
2016 ◽  
Vol 16 (2) ◽  
pp. 269-281 ◽  
Author(s):  
V. Georgi ◽  
L. Georgi ◽  
M. Blechert ◽  
M. Bergmeister ◽  
M. Zwanzig ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Microfluidic Device ◽  
Free Protein ◽  
On Chip ◽  
Cell Free Protein Synthesis

The microfluidic device for cell-free protein synthesis enables a novel reaction mode with continuous in vitro transcription and translation in separate compartments combined by magnetic and microfluidic actuated mRNA transfer.

scholarly journals Development and comparison of cell-free protein synthesis systems derived from typical bacterial chassis

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Liyuan Zhang ◽  
Xiaomei Lin ◽  
Ting Wang ◽  
Wei Guo ◽  
Yuan Lu
Keyword(s):  
Protein Synthesis ◽  
Protein Production ◽  
Influential Factors ◽  
Competent Cell ◽  
Free Protein ◽  
Application Range ◽  
E Coli ◽  
Short Time ◽  
Cell Free Protein Synthesis

AbstractCell-free protein synthesis (CFPS) systems have become an ideal choice for pathway prototyping, protein production, and biosensing, due to their high controllability, tolerance, stability, and ability to produce proteins in a short time. At present, the widely used CFPS systems are mainly based on Escherichia coli strain. Bacillus subtilis, Corynebacterium glutamate, and Vibrio natriegens are potential chassis cells for many biotechnological applications with their respective characteristics. Therefore, to expand the platform of the CFPS systems and options for protein production, four prokaryotes, E. coli, B. subtilis, C. glutamate, and V. natriegens were selected as host organisms to construct the CFPS systems and be compared. Moreover, the process parameters of the CFPS system were optimized, including the codon usage, plasmid synthesis competent cell selection, plasmid concentration, ribosomal binding site (RBS), and CFPS system reagent components. By optimizing and comparing the main influencing factors of different CFPS systems, the systems can be optimized directly for the most influential factors to further improve the protein yield of the systems. In addition, to demonstrate the applicability of the CFPS systems, it was proved that the four CFPS systems all had the potential to produce therapeutic proteins, and they could produce the receptor-binding domain (RBD) protein of SARS-CoV-2 with functional activity. They not only could expand the potential options for in vitro protein production, but also could increase the application range of the system by expanding the cell-free protein synthesis platform.

Chemical IC Chip Family for on-Chip Cell-Free Protein Synthesis From DNA

2001 ◽  
pp. 66-68 ◽  
Author(s):  
Koji Ikuta ◽  
Kota Ikeda ◽  
Atsushi Takahashi ◽  
Shoji Maruo
Keyword(s):  
Protein Synthesis ◽  
Free Protein ◽  
On Chip ◽  
Cell Free Protein Synthesis

scholarly journals In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis

2016 ◽  
Vol 6 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Anthony W. Goering ◽  
Jian Li ◽  
Ryan A. McClure ◽  
Regan J. Thomson ◽  
Michael C. Jewett ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Nonribosomal Peptide ◽  
Free Protein ◽  
Peptide Biosynthesis ◽  
Cell Free Protein Synthesis

scholarly journals <italic>In vitro</italic> synthetic biology: Cell-free protein synthesis

2017 ◽  
Vol 62 (33) ◽  
pp. 3851-3860
Author(s):  
Yang LIU ◽  
XiaoCui GUO ◽  
JinHui GENG ◽  
Yi JIAO ◽  
JinPeng HAN ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Synthetic Biology ◽  
Free Protein ◽  
Cell Free Protein Synthesis

scholarly journals Two fractions required for cell-free protein synthesis with components from rabbit reticulocytes

1969 ◽  
Vol 115 (3) ◽  
pp. 523-527 ◽  
Author(s):  
Brian B. Cohen
Keyword(s):  
Protein Synthesis ◽  
Active Component ◽  
Sedimentation Coefficient ◽  
Sucrose Density Gradient ◽  
Analytical Ultracentrifuge ◽  
Active Components ◽  
Free Protein ◽  
Cell Free Protein Synthesis ◽  
Rabbit Reticulocytes

An extract was prepared from rabbit reticulocyte ribosomes after treatment with potassium chloride as described by Miller, Hamada, Yang, Cohen & Schweet (1967). This extract has been shown to convert monoribosomes into polyribosomes during protein synthesis in vitro (Cohen, 1968). The nature of this extract was studied in greater detail. Centrifugation of the extract through a sucrose density gradient separated the activity into a fast-sedimenting fraction. The two fractions were shown to have different functions in stimulating cell-free protein synthesis and their active components were shown to be protein or partly protein in nature. Each fraction was analysed by electrophoresis and in the analytical ultracentrifuge. It was concluded that the active component in the fast-sedimenting fraction had a sedimentation coefficient of 15·5s and that of the slow-sedimenting fraction 10·5s.

Sign in / Sign up

Export Citation Format

Share Document