High throughput cryopreservation of cells by rapid freezing of sub-μl drops using inkjet printing – cryoprinting

Lab on a Chip ◽  
2015 ◽  
Vol 15 (17) ◽  
pp. 3503-3513 ◽  
Author(s):  
Rui Dou ◽  
Rachel E. Saunders ◽  
Lisa Mohamet ◽  
Christopher M. Ward ◽  
Brian Derby
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Throughput ◽  
Human Embryonic Stem Cells ◽  
Inkjet Printing ◽  
Embryonic Stem ◽  
Mouse Fibroblast ◽  
Rapid Freezing ◽  
Fibroblast Cells

We have successfully used inkjet printing to cryopreserve 3T3 mouse fibroblast cells and human neuroprogenitor cells (NPCs) derived from human embryonic stem cells (hESCs).

scholarly journals High-Throughput Screening Assay for the Identification of Compounds Regulating Self-Renewal and Differentiation in Human Embryonic Stem Cells

2008 ◽  
Vol 2 (6) ◽  
pp. 602-612 ◽  
Author(s):  
Sabrina C. Desbordes ◽  
Dimitris G. Placantonakis ◽  
Anthony Ciro ◽  
Nicholas D. Socci ◽  
Gabsang Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Throughput ◽  
Human Embryonic Stem Cells ◽  
High Throughput Screening ◽  
Embryonic Stem ◽  
Screening Assay ◽  
Self Renewal ◽  
High Throughput Screening Assay

scholarly journals Efficient, high-throughput transfection of human embryonic stem cells

2010 ◽  
Vol 1 (3) ◽  
pp. 23 ◽  
Author(s):  
Jennifer C Moore ◽  
Kristin Atze ◽  
Percy L Yeung ◽  
Alana J Toro-Ramos ◽  
Cynthia Camarillo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Throughput ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem

scholarly journals StemCell Keep™ is Effective for Cryopreservation of Human Embryonic Stem Cells by Vitrification

2017 ◽  
Vol 26 (5) ◽  
pp. 773-787 ◽  
Author(s):  
Akemi Ota ◽  
Kazuaki Matsumura ◽  
Jun-Jae Lee ◽  
Shoichiro Sumi ◽  
Soung-Hyu Hyon
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Number ◽  
Mouse Fibroblast ◽  
Stem Cell Markers ◽  
Rt Pcr ◽  
Induced Pluripotent ◽  
Attachment Ability

Safe and stable cryopreservation is critical for research involving human embryonic stem cells (hESCs). Dimethyl sulfoxide (DMSO) is a popular cryoprotective agent; however, its cytotoxicity cannot be ignored. Thus, there is a need for an alternate cryoprotectant. We reported previously that a novel cryopreservation reagent, StemCell Keep™ (SCK), was effective for cryopreserving human induced pluripotent stem cells (hiPSCs) by vitrification. Because hESCs and hiPSCs are not identical, the current study examined the use of SCK on hESCs. hESCs cryopreserved with SCK were thawed and cultured on SNL 76/7 cells, which were derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes. After cryopreservation, cultured hESCs were assessed for their attachment ability and characterized by alkaline phosphatase (AP) and immunocytochemical (ICC) staining, fluorescence-activated cell sorting (FACS), reverse transcription polymerase chain reaction (RT-PCR), and karyotyping. The proliferation of SCK-cryopreserved hESCs cultured on SNL cells, or in feeder-free conditions, was higher than that of cells preserved in a solution of 2 M DMSO, 1 M acetamide, and 3 M propylene glycol (DAP). The cell number with SCK-cryopreserved hESCs was about twice that of hESCs cryopreserved in DAP. The pluripotency of SCK-cryopreserved hESCs was similar to that of DAP-cryopreserved hESCs based on AP staining. Data from ICC, FACS, and RT-PCR analyses showed that stem cell markers were continually expressed on SCK-cryopreserved hESCs. The teratoma assay showed that SCK-cryopreserved hESCs differentiated into three germ layers. Furthermore, SCK-cryopreserved hESCs had normal karyotypes. These data indicate that SCK was effective for cryopreservation of hESCs by vitrification.

47. Towards a scalable, high-throughput cryopreservation strategy for human embryonic stem cells

Cryobiology ◽  
2010 ◽  
Vol 61 (3) ◽  
pp. 376
Author(s):  
Rita Malpique ◽  
Margarida Serra ◽  
Catarina Brito ◽  
Paula M. Alves
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Throughput ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem

scholarly journals A high-throughput multiplexed screening assay for optimizing serum-free differentiation protocols of human embryonic stem cells

2011 ◽  
Vol 6 (2) ◽  
pp. 129-142 ◽  
Author(s):  
Joel T. Outten ◽  
Xin Cheng ◽  
Paul Gadue ◽  
Deborah L. French ◽  
Scott L. Diamond
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Throughput ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Screening Assay ◽  
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