The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

Lab on a Chip ◽  
2015 ◽  
Vol 15 (17) ◽  
pp. 3439-3459 ◽  
Author(s):  
David J. Collins ◽  
Adrian Neild ◽  
Andrew deMello ◽  
Ai-Qun Liu ◽  
Ye Ai
Keyword(s):  
Single Cell ◽  
Poisson Distribution ◽  
State Of The Art ◽  
Cell Encapsulation ◽  
The State ◽  
Droplet Production ◽  
Single Cell Encapsulation

In recent years there has been an explosion of methods for encapsulating cells in droplets. This review examines the state-of-the-art, including methods for active encapsulation.

scholarly journals Geometry Effects of Axisymmetric Flow-Focusing Microchannels for Single Cell Encapsulation

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2811 ◽  
Author(s):  
Mohammad Nooranidoost ◽  
Ranganathan Kumar
Keyword(s):  
Single Cell ◽  
Axisymmetric Flow ◽  
Cell Encapsulation ◽  
Droplet Formation ◽  
Flow Focusing ◽  
Front Tracking Method ◽  
Droplet Production ◽  
Geometry Effects ◽  
Single Cell Encapsulation ◽  
Number Of Cells

Cell microencapsulation is a promising technique to protect living cells in biomedical applications. Microfluidic devices can be utilized to control the production of high-throughput cell-laden droplets. This paper demonstrates the effects of flow-focusing geometry on the droplet size, frequency of droplet generation, and number of cells per droplet. Orifice radius, orifice length, and nozzle-to-orifice distance can significantly influence the flow-field and manipulate droplet formation. This paper analyzes these geometry effects using a numerical front-tracking method for the three fluid phases. It is found that as the orifice radius increases, the drop size and the number of cells in the droplet increase. For a short orifice radius, increasing the orifice length results in the generation of smaller droplets at higher frequency and fewer cells per droplet. On the other hand, for a longer orifice, droplet production is invariant with respect to orifice length. It is also found that shorter distances between the nozzle and the orifice lead to a more controlled and uniform production of droplets. When the nozzle-to-orifice length is increased, the droplet formation becomes non-uniform and unpredictable. Probability charts are plotted with respect to the orifice length and orifice radius, which show that a greater than 50 % probability of single cell encapsulation can be achieved consistently.

scholarly journals Active single cell encapsulation using SAW overcoming the limitations of Poisson distribution

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Andreas Link ◽  
John S McGrath ◽  
Mustafa Zaimagaoglu ◽  
Thomas Franke
Keyword(s):  
Red Blood Cells ◽  
Single Cell ◽  
Poisson Distribution ◽  
Blood Cells ◽  
Cell Encapsulation ◽  
Acoustic Device ◽  
Single Cell Encapsulation

We demonstrate the use of an acoustic device to actively encapsulate single red blood cells into individual droplets in a T-junction. We compare the active encapsulation with the passive encapsulation...

High-throughput deterministic single-cell encapsulation and droplet pairing, fusion, and shrinkage in a single microfluidic device

2013 ◽  
Vol 35 (2-3) ◽  
pp. 385-392 ◽  
Author(s):  
Rogier M. Schoeman ◽  
Evelien W.M. Kemna ◽  
Floor Wolbers ◽  
Albert van den Berg
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
High Throughput ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation

SINGLE CELL ENCAPSULATION IMPROVES STEM CELL RETENTION AND CELL-MEDIATED CARDIAC REPAIR

2014 ◽  
Vol 30 (10) ◽  
pp. S58 ◽  
Author(s):  
A.E. Mayfield ◽  
E.L. Tilokee ◽  
N. Latham ◽  
F. Rubens ◽  
D.W. Courtman ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Cell Encapsulation ◽  
Cardiac Repair ◽  
Cell Retention ◽  
Single Cell Encapsulation

Micropillar array embedded system for single cell encapsulation in hydrogel

2015 ◽  
Vol 1724 ◽  
Author(s):  
Kyun Joo Park ◽  
Kyoung G. Lee ◽  
Seunghwan Seok ◽  
Bong Gill Choi ◽  
Seok Jae Lee ◽  
...  
Keyword(s):  
Embedded System ◽  
Single Cell ◽  
Single Cells ◽  
Cell Encapsulation ◽  
Polyethylene Glycol Diacrylate ◽  
Glycol Diacrylate ◽  
Practical Applications ◽  
Cell Clusters ◽  
Single Cell Encapsulation ◽  
Micropillar Array

ABSTRACTA cylindrical-shaped micropillar array embedded microfluidic device was proposed to enhance the dispersion of cell clusters and the efficiency of single cell encapsulation in hydrogel. Different sizes of micropillar arrays act as a sieve to break Escherichia coli (E. coli) aggregates into single cells in polyethylene glycol diacrylate (PEGDA) solution. We applied the external force for the continuous breakup of cell clusters, resulting in the production of more than 70% of single cells into individual hydrogel particles. This proposed strategy and device will be a useful platform to utilize genetically modified microorganisms in practical applications.

Peptide-catalyzed, bioinspired silicification for single-cell encapsulation in the imidazole-buffered system

2015 ◽  
Vol 51 (25) ◽  
pp. 5523-5525 ◽  
Author(s):  
Ji Hun Park ◽  
Insung S. Choi ◽  
Sung Ho Yang
Keyword(s):  
Single Cell ◽  
Cell Encapsulation ◽  
Yeast Cells ◽  
Mild Conditions ◽  
System P ◽  
Glass Sponges ◽  
Silica Encapsulation ◽  
Single Cell Encapsulation

Cytoprotective silica encapsulation of individual yeast cells is achieved by a peptide-catalyzed silicification under mild conditions, inspired by glass sponges.

scholarly journals Deterministic trapping, encapsulation and retrieval of single-cells

Lab on a Chip ◽  
2017 ◽  
Vol 17 (13) ◽  
pp. 2186-2192 ◽  
Author(s):  
M. Sauzade ◽  
E. Brouzes
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Cell Encapsulation ◽  
Single Cell Encapsulation ◽  
Passive Strategy

Passive strategy for efficient true single-cell encapsulation.

scholarly journals Multiplexed Immunohistochemistry and Digital Pathology as the Foundation for Next-Generation Pathology in Melanoma: Methodological Comparison and Future Clinical Applications

2021 ◽  
Vol 11 ◽  
Author(s):  
Yannick Van Herck ◽  
Asier Antoranz ◽  
Madhavi Dipak Andhari ◽  
Giorgia Milli ◽  
Oliver Bechter ◽  
...  
Keyword(s):  
Artificial Intelligence ◽  
Single Cell ◽  
State Of The Art ◽  
Deep Understanding ◽  
Response Rates ◽  
The State ◽  
Patient Specific ◽  
Specific Cell ◽  
Next Generation ◽  
Single Cell Profiling

The state-of-the-art for melanoma treatment has recently witnessed an enormous revolution, evolving from a chemotherapeutic, “one-drug-for-all” approach, to a tailored molecular- and immunological-based approach with the potential to make personalized therapy a reality. Nevertheless, methods still have to improve a lot before these can reliably characterize all the tumoral features that make each patient unique. While the clinical introduction of next-generation sequencing has made it possible to match mutational profiles to specific targeted therapies, improving response rates to immunotherapy will similarly require a deep understanding of the immune microenvironment and the specific contribution of each component in a patient-specific way. Recent advancements in artificial intelligence and single-cell profiling of resected tumor samples are paving the way for this challenging task. In this review, we provide an overview of the state-of-the-art in artificial intelligence and multiplexed immunohistochemistry in pathology, and how these bear the potential to improve diagnostics and therapy matching in melanoma. A major asset of in-situ single-cell profiling methods is that these preserve the spatial distribution of the cells in the tissue, allowing researchers to not only determine the cellular composition of the tumoral microenvironment, but also study tissue sociology, making inferences about specific cell-cell interactions and visualizing distinctive cellular architectures - all features that have an impact on anti-tumoral response rates. Despite the many advantages, the introduction of these approaches requires the digitization of tissue slides and the development of standardized analysis pipelines which pose substantial challenges that need to be addressed before these can enter clinical routine.

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