Partitioned EDGE devices for high throughput production of monodisperse emulsion droplets with two distinct sizes

Lab on a Chip ◽  
2015 ◽  
Vol 15 (11) ◽  
pp. 2486-2495 ◽  
Author(s):  
Sami Sahin ◽  
Karin Schroën
Keyword(s):  
High Throughput ◽  
Droplet Generation ◽  
Emulsion Droplets ◽  
Monodisperse Emulsion ◽  
Edge Based

We present a novel microfluidic EDGE (Edge based Droplet GEneration) device with regularly spaced micron-sized partitions, which is aimed at upscaling of o/w emulsion preparation.

Droplet-based microfluidics in biomedical applications

2021 ◽  
Author(s):  
Leyla Amirifar ◽  
Mohsen Besanjideh ◽  
Rohollah Nasiri ◽  
Amir Shamloo ◽  
Fatemeh Nasrollahi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
High Throughput ◽  
Paradigm Shift ◽  
Cell Biology ◽  
Biomedical Applications ◽  
Molecular Detection ◽  
Droplet Microfluidics ◽  
Droplet Generation ◽  
Microfluidic Platforms ◽  
Standard Methods

Abstract Droplet-based microfluidic systems have been employed to manipulate discrete fluid volumes with immiscible phases. Creating the fluid droplets at microscale has led to a paradigm shift in mixing, sorting, encapsulation, sensing, and designing high throughput devices for biomedical applications. Droplet microfluidics has opened many opportunities in microparticle synthesis, molecular detection, diagnostics, drug delivery, and cell biology. In the present review, we first introduce standard methods for droplet generation (i.e., passive and active methods) and discuss the latest examples of emulsification and particle synthesis approaches enabled by microfluidic platforms. Then, the applications of droplet-based microfluidics in different biomedical applications are detailed. Finally, a general overview of the latest trends along with the perspectives and future potentials in the field are provided.

Oscillating dispersed-phase co-flow microfluidic droplet generation: jet length reduction effect

Soft Matter ◽  
2018 ◽  
Vol 14 (48) ◽  
pp. 9870-9876 ◽  
Author(s):  
Amin Shams Khorrami ◽  
Pouya Rezai
Keyword(s):  
High Throughput ◽  
Droplet Generation ◽  
Dispersed Phase ◽  
Jet Length ◽  
Reduction Effect ◽  
Length Reduction

An oscillating-needle co-flow technique for jet length reduction and production of multi-size droplets at high throughput in a channel.

scholarly journals Double emulsion flow cytometry with high-throughput single droplet isolation and nucleic acid recovery

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2062-2074 ◽  
Author(s):  
Kara K. Brower ◽  
Catherine Carswell-Crumpton ◽  
Sandy Klemm ◽  
Bianca Cruz ◽  
Gaeun Kim ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
High Throughput ◽  
Double Emulsion ◽  
Single Droplet ◽  
Emulsion Droplets ◽  
Acid Recovery ◽  
Emulsion Flow ◽  
Double Emulsion Droplets

We have developed a novel workflow (sdDE-FACS, s̲ingle d̲roplet D̲ouble E̲mulsion FACS) that allows robust production, screening, and sorting of single double emulsion droplets with complete nucleic acid recovery.

scholarly journals Front Cover: Rapid automatic creation of monodisperse emulsion droplets by microfluidic device with degassed PDMS slab as a detachable suction actuator

2018 ◽  
Vol 39 (3) ◽  
pp. NA-NA
Author(s):  
Yuki Murata ◽  
Yuta Nakashoji ◽  
Masaki Kondo ◽  
Yugo Tanaka ◽  
Masahiko Hashimoto
Keyword(s):  
Microfluidic Device ◽  
Front Cover ◽  
Emulsion Droplets ◽  
Monodisperse Emulsion

Straight-through microchannel devices for generating monodisperse emulsion droplets several microns in size

2007 ◽  
Vol 4 (3) ◽  
pp. 167-177 ◽  
Author(s):  
Isao Kobayashi ◽  
Takayuki Takano ◽  
Ryutaro Maeda ◽  
Yoshihiro Wada ◽  
Kunihiko Uemura ◽  
...  
Keyword(s):  
Emulsion Droplets ◽  
Monodisperse Emulsion ◽  
Microchannel Devices

scholarly journals Simultaneous Droplet Generation with In-Series Droplet T-Junctions Induced by Gravity-Induced Flow

Micromachines ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1211
Author(s):  
Khashayar Bajgiran ◽  
Alejandro Cordova ◽  
Riad Elkhanoufi ◽  
James Dorman ◽  
Adam Melvin
Keyword(s):  
Single Cell ◽  
High Throughput ◽  
Droplet Diameter ◽  
Droplet Microfluidics ◽  
Droplet Generation ◽  
Single Experiment ◽  
Flow Rates ◽  
Wide Range ◽  
Significant Difference ◽  
Gravity Driven Flow

Droplet microfluidics offers a wide range of applications, including high-throughput drug screening and single-cell DNA amplification. However, these platforms are often limited to single-input conditions that prevent them from analyzing multiple input parameters (e.g., combined cellular treatments) in a single experiment. Droplet multiplexing will result in higher overall throughput, lowering cost of fabrication, and cutting down the hands-on time in number of applications such as single-cell analysis. Additionally, while lab-on-a-chip fabrication costs have decreased in recent years, the syringe pumps required for generating droplets of uniform shape and size remain cost-prohibitive for researchers interested in utilizing droplet microfluidics. This work investigates the potential of simultaneously generating droplets from a series of three in-line T-junctions utilizing gravity-driven flow to produce consistent, well-defined droplets. Implementing reservoirs with equal heights produced inconsistent flow rates that increased as a function of the distance between the aqueous inlets and the oil inlet. Optimizing the three reservoir heights identified that taller reservoirs were needed for aqueous inlets closer to the oil inlet. Studying the relationship between the ratio of oil-to-water flow rates () found that increasing resulted in smaller droplets and an enhanced droplet generation rate. An ANOVA was performed on droplet diameter to confirm no significant difference in droplet size from the three different aqueous inlets. The work described here offers an alternative approach to multiplexed droplet microfluidic devices allowing for the high-throughput interrogation of three sample conditions in a single device. It also has provided an alternative method to induce droplet formation that does not require multiple syringe pumps.

Design of high throughput droplet generation chip

2020 ◽  
Vol 28 (12) ◽  
pp. 2636-2645
Author(s):  
Wu-ping ZHOU ◽  
◽  
Yu-guo TANG ◽  
Hai-wen LI ◽  
Ke-ming JIANG ◽  
...  
Keyword(s):  
High Throughput ◽  
Droplet Generation

On-Demand Production of Emulsion Droplets Using Magnetically Driven Microtool

2009 ◽  
Vol 3 (5) ◽  
pp. 502-508 ◽  
Author(s):  
Yoko Yamanishi ◽  
◽  
Yuki Kihara ◽  
Shinya Sakuma ◽  
Fumihito Arai
Keyword(s):  
External Force ◽  
Parallel Plate ◽  
Droplet Generation ◽  
Hybrid Polymer ◽  
On Demand ◽  
Emulsion Droplets ◽  
Specific Direction ◽  
Magnetic Metal ◽  
Polymer Metal

We propose a hybrid polymer-metal magnetically driven microtool (MMT) featuring elasticity and rigidity. An electroplated magnetic metal axle is installed directly at the center of the MMT during molding. The result is a hybrid MMT whose fixed axes move elastically in a specific direction and whose center axle is rigid, preventing bending by unwanted external force. The axle’s higher magnetism contributes to powerful actuation. The hybrid MMT we designed provides on-demand droplet dispensation on chips. Its parallel plate is constrained translationally. Hybrid MMT displacement is 300 μm - 6 times greater than that of the conventional MMT. On-demand droplet generation produces a 177.7 ±2.3 μm droplet.

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