Size and deformability based separation of circulating tumor cells from castrate resistant prostate cancer patients using resettable cell traps

Lab on a Chip ◽  
2015 ◽  
Vol 15 (10) ◽  
pp. 2278-2286 ◽  
Author(s):  
Xi Qin ◽  
Sunyoung Park ◽  
Simon P. Duffy ◽  
Kerryn Matthews ◽  
Richard R. Ang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Spectral Analysis ◽  
Single Cell ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Castrate Resistant Prostate Cancer ◽  
Castrate Resistant

Separation of CTCs using resettable cell traps followed by single-cell spectral analysis.

scholarly journals Detection of AR-V7 in Liquid Biopsies of Castrate Resistant Prostate Cancer Patients: A Comparison of AR-V7 Analysis in Circulating Tumor Cells, Circulating Tumor RNA and Exosomes

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 688 ◽  
Author(s):  
Mohammed Nimir ◽  
Yafeng Ma ◽  
Sarah A. Jeffreys ◽  
Thomas Opperman ◽  
Francis Young ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Digital Pcr ◽  
Liquid Biopsies ◽  
Tumor Biopsy ◽  
Highly Sensitive ◽  
Castrate Resistant Prostate Cancer ◽  
Castrate Resistant

Detection of androgen receptor (AR) variant 7 (AR-V7) is emerging as a clinically important biomarker in castrate resistant prostate cancer (CRPC). Detection is possible from tumor tissue, which is often inaccessible in the advanced disease setting. With recent progress in detecting AR-V7 in circulating tumor cells (CTCs), circulating tumor RNA (ctRNA) and exosomes from prostate cancer patients, liquid biopsies have emerged as an alternative to tumor biopsy. Therefore, it is important to clarify whether these approaches differ in sensitivity in order to achieve the best possible biomarker characterization for the patient. In this study, blood samples from 44 prostate cancer patients were processed for CTCs and ctRNA with subsequent AR-V7 testing, while exosomal RNA was isolated from 16 samples and tested. Detection of AR and AR-V7 was performed using a highly sensitive droplet digital PCR-based assay. AR and AR-V7 RNA were detectable in CTCs, ctRNA and exosome samples. AR-V7 detection from CTCs showed higher sensitivity and has proven specificity compared to detection from ctRNA and exosomes. Considering that CTCs are almost always present in the advanced prostate cancer setting, CTC samples should be considered the liquid biopsy of choice for the detection of this clinically important biomarker.

scholarly journals Large Extracellular Vesicle Characterization and Association with Circulating Tumor Cells in Metastatic Castrate Resistant Prostate Cancer

Cancers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1056
Author(s):  
Anna S. Gerdtsson ◽  
Sonia M. Setayesh ◽  
Paymaneh D. Malihi ◽  
Carmen Ruiz ◽  
Anders Carlsson ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Extracellular Vesicle ◽  
Object Identification ◽  
Protein Profiling ◽  
Liquid Biopsies ◽  
Cancer Tumor ◽  
Castrate Resistant Prostate Cancer ◽  
Castrate Resistant

Liquid biopsies hold potential as minimally invasive sources of tumor biomarkers for diagnosis, prognosis, therapy prediction or disease monitoring. We present an approach for parallel single-object identification of circulating tumor cells (CTCs) and tumor-derived large extracellular vesicles (LEVs) based on automated high-resolution immunofluorescence followed by downstream multiplexed protein profiling. Identification of LEVs >6 µm in size and CTC enumeration was highly correlated, with LEVs being 1.9 times as frequent as CTCs, and additional LEVs were identified in 73% of CTC-negative liquid biopsy samples from metastatic castrate resistant prostate cancer. Imaging mass cytometry (IMC) revealed that 49% of cytokeratin (CK)-positive LEVs and CTCs were EpCAM-negative, while frequently carrying prostate cancer tumor markers including AR, PSA, and PSMA. HSPD1 was shown to be a specific biomarker for tumor derived circulating cells and LEVs. CTCs and LEVs could be discriminated based on size, morphology, DNA load and protein score but not by protein signatures. Protein profiles were overall heterogeneous, and clusters could be identified across object classes. Parallel analysis of CTCs and LEVs confers increased sensitivity for liquid biopsies and expanded specificity with downstream characterization. Combined, it raises the possibility of a more comprehensive assessment of the disease state for precise diagnosis and monitoring.

Sign in / Sign up

Export Citation Format

Share Document