scholarly journals High-resolution CMOS MEA platform to study neurons at subcellular, cellular, and network levels

Lab on a Chip ◽  
2015 ◽  
Vol 15 (13) ◽  
pp. 2767-2780 ◽  
Author(s):  
Jan Müller ◽  
Marco Ballini ◽  
Paolo Livi ◽  
Yihui Chen ◽  
Milos Radivojevic ◽  
...  

Novel CMOS-based microelectrode array to enable high-spatiotemporal- resolution access to neuronal preparations on subcellular, cellular, and network level.

Author(s):  
Rüdiger Stirnberg ◽  
Tony Stöcker

AbstractPurposeA segmented k-space blipped-CAIPI (skipped-CAIPI) sampling strategy for echo planar imaging (EPI) is proposed, which allows for a flexible choice of EPI factor and phase encode bandwidth independent of the controlled aliasing (CAIPIRINHA) pattern.Theory and MethodsWith previously proposed approaches, exactly two EPI trajectories were possible given a specific CAIPIRINHA pattern: either with slice gradient blips (blipped-CAIPI), or following a shot-selective approach (higher resolution). Recently, interleaved multi-shot segmentation along shot-selective CAIPI trajectories has been applied for high-resolution anatomical imaging. For more flexibility and a broader range of applications, we propose segmentation along any blipped-CAIPI trajectory. Thus, all EPI factors and phase encode bandwidths available with traditional segmented EPI can be combined with controlled aliasing.ResultsTemporal signal-to-noise ratios of moderate-to-high-resolution time series acquisitions at varying undersampling factors demonstrate beneficial sampling alternatives to blipped-CAIPI or shot-selective CAIPI. Rapid high-resolution scans furthermore demonstrate SNR-efficient and motion-robust structural imaging with almost arbitrary EPI factor and minimal noise penalty.ConclusionsSkipped-CAIPI sampling increases protocol flexibility for high spatiotemporal resolution EPI. In terms of signal-to-noise ratio and efficiency, high-resolution functional or structural scans benefit vastly from a free choice of the CAIPIRINHA pattern. Even at moderate resolutions, the independence of sampling pattern, echo time and image matrix size is valuable for optimized functional protocol design. Although demonstrated with 3D-EPI, skipped-CAIPI is also applicable with simultaneous multislice EPI.


2018 ◽  
Vol 217 (5) ◽  
pp. 1869-1882 ◽  
Author(s):  
Tanner C. Fadero ◽  
Therese M. Gerbich ◽  
Kishan Rana ◽  
Aussie Suzuki ◽  
Matthew DiSalvo ◽  
...  

Fluorescence microscopy is a powerful approach for studying subcellular dynamics at high spatiotemporal resolution; however, conventional fluorescence microscopy techniques are light-intensive and introduce unnecessary photodamage. Light-sheet fluorescence microscopy (LSFM) mitigates these problems by selectively illuminating the focal plane of the detection objective by using orthogonal excitation. Orthogonal excitation requires geometries that physically limit the detection objective numerical aperture (NA), thereby limiting both light-gathering efficiency (brightness) and native spatial resolution. We present a novel live-cell LSFM method, lateral interference tilted excitation (LITE), in which a tilted light sheet illuminates the detection objective focal plane without a sterically limiting illumination scheme. LITE is thus compatible with any detection objective, including oil immersion, without an upper NA limit. LITE combines the low photodamage of LSFM with high resolution, high brightness, and coverslip-based objectives. We demonstrate the utility of LITE for imaging animal, fungal, and plant model organisms over many hours at high spatiotemporal resolution.


Lab on a Chip ◽  
2015 ◽  
Vol 15 (20) ◽  
pp. 4075-4082 ◽  
Author(s):  
John B. Wydallis ◽  
Rachel M. Feeny ◽  
William Wilson ◽  
Tucker Kern ◽  
Tom Chen ◽  
...  

Electrochemical imaging with high spatiotemporal resolution of dynamic norepinephrine distributions is achieved using microfluidics and a high-density CMOS platinum microelectrode array with an on-board potentiostat.


2021 ◽  
Vol 13 (5) ◽  
pp. 954
Author(s):  
Abhilash K. Chandel ◽  
Lav R. Khot ◽  
Behnaz Molaei ◽  
R. Troy Peters ◽  
Claudio O. Stöckle ◽  
...  

Site-specific irrigation management for perennial crops such as grape requires water use assessments at high spatiotemporal resolution. In this study, small unmanned-aerial-system (UAS)-based imaging was used with a modified mapping evapotranspiration at high resolution with internalized calibration (METRIC) energy balance model to map water use (UASM-ET approach) of a commercial, surface, and direct-root-zone (DRZ) drip-irrigated vineyard. Four irrigation treatments, 100%, 80%, 60%, and 40%, of commercial rate (CR) were also applied, with the CR estimated using soil moisture data and a non-stressed average crop coefficient of 0.5. Fourteen campaigns were conducted in the 2018 and 2019 seasons to collect multispectral (ground sampling distance (GSD): 7 cm/pixel) and thermal imaging (GSD: 13 cm/pixel) data. Six of those campaigns were near Landsat 7/8 satellite overpass of the field site. Weather inputs were obtained from a nearby WSU-AgWeatherNet station (1 km). First, UASM-ET estimates were compared to those derived from soil water balance (SWB) and conventional Landsat-METRIC (LM) approaches. Overall, UASM-ET (2.70 ± 1.03 mm day−1 [mean ± std. dev.]) was higher than SWB-ET (1.80 ± 0.98 mm day−1). However, both estimates had a significant linear correlation (r = 0.64–0.81, p < 0.01). For the days of satellite overpass, UASM-ET was statistically similar to LM-ET, with mean absolute normalized ET departures (ETd,MAN) of 4.30% and a mean r of 0.83 (p < 0.01). The study also extracted spatial canopy transpiration (UASM-T) maps by segmenting the soil background from the UASM-ET, which had strong correlation with the estimates derived by the standard basal crop coefficient approach (Td,MAN = 14%, r = 0.95, p < 0.01). The UASM-T maps were then used to quantify water use differences in the DRZ-irrigated grapevines. Canopy transpiration (T) was statistically significant among the irrigation treatments and was highest for grapevines irrigated at 100% or 80% of the CR, followed by 60% and 40% of the CR (p < 0.01). Reference T fraction (TrF) curves established from the UASM-T maps showed a notable effect of irrigation treatment rates. The total water use of grapevines estimated using interpolated TrF curves was highest for treatments of 100% (425 and 320 mm for the 2018 and 2019 seasons, respectively), followed by 80% (420 and 317 mm), 60% (391 and 318 mm), and 40% (370 and 304 mm) of the CR. Such estimates were within 5% to 11% of the SWB-based water use calculations. The UASM-T-estimated water use was not the same as the actual amount of water applied in the two seasons, probably because DRZ-irrigated vines might have developed deeper or lateral roots to fulfill water requirements outside the irrigated soil volume. Overall, results highlight the usefulness of high-resolution imagery toward site-specific water use management of grapevines.


2015 ◽  
Vol 149 ◽  
pp. 86-94 ◽  
Author(s):  
Dimitar R Stamov ◽  
Erik Stock ◽  
Clemens M Franz ◽  
Torsten Jähnke ◽  
Heiko Haschke

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