Magnetic bead-based AuNP labelling combined with inductively coupled plasma mass spectrometry for sensitively and specifically counting cancer cells

2016 ◽  
Vol 31 (3) ◽  
pp. 679-685 ◽  
Author(s):  
WeiJuan Yang ◽  
Zhiming Xi ◽  
Xiaoxue Zeng ◽  
Ling Fang ◽  
Wenjing Jiang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cancer Cells ◽  
Inductively Coupled Plasma ◽  
Magnetic Bead ◽  
Labelling Technique ◽  
Inductively Coupled ◽  
Ms Detection ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Novel Method

A novel method for sensitively and specifically counting cancer cells using a MB-based AuNP–aptamer labelling technique and ICP-MS detection is presented in this study.

Uptake of antitumor platinum(II)-complexes by cancer cells, assayed by inductively coupled plasma mass spectrometry (ICP-MS)

2004 ◽  
Vol 98 (1) ◽  
pp. 73-78 ◽  
Author(s):  
AnnaRita Ghezzi ◽  
Maurizio Aceto ◽  
Claudio Cassino ◽  
Elisabetta Gabano ◽  
Domenico Osella
Keyword(s):  
Mass Spectrometry ◽  
Cancer Cells ◽  
Inductively Coupled Plasma ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

scholarly journals Study of magnesium bioavailability using stable isotopes and the inductively-coupled plasma mass spectrometry technique in the rat: single and double labelling approaches

1997 ◽  
Vol 77 (6) ◽  
pp. 957-970 ◽  
Author(s):  
Charles Coudray ◽  
Denise Pepin ◽  
Jean Claude Tressol ◽  
Jacques Bellanger ◽  
Yves Rayssiguier
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotopes ◽  
Inductively Coupled Plasma ◽  
Double Labelling ◽  
Adaptation Period ◽  
Labelling Technique ◽  
Inductively Coupled ◽  
True Absorption ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

The present work aimed to investigate the feasibility of using stable isotopes and inductively-coupled plasma mass spectrometry (ICP/MS) to study Mg absorption in rats. Male Wistar rats, aged 7 weeks and weighing 180g, were used. They were fed on a semi-purified diet containing 1070mg Mg/kg, and had free access to feed and distilled water. In the first experiment, after a 16d adaptation period, two doses of enriched 25Mg (6 and 12mg) were administered by oral intubation, faeces and urine were collected daily and blood was sampled. Isotope ratios were determined by ICP/;MS. ‘True’ absorption values, using the faecal isotope data, were 0.63 and 0.56 in rats receiving 6 and 12mg 25Mg respectively, while apparent absorption was 0.50 for two successive periods of metabolic balance studies. Moreover, the oral isotope administration resulted in a measurable isotopic enrichment in plasma within hours which was still detectable on the third day following the isotope administration. In the second experiment, investigating the double labelling technique, similar rats were dosed Simultaneously with 5mg 26Mg orally (premixed with diet) and 0.29mg 25Mg intravenously. The calculated Mg true absorption values were very similar when calculated from blood or urine data (0.38) but were lower than that obtained from faecal data (0.50). The possible causes of such an unexpected difference and limits of the double labelling technique for Mg absorption are discussed here. Together these results indicate that although 25Mg and 26Mg isotopes have high natural abundance, the described methodology permits meaningful investigations of Mg bioavailability and metabolism

scholarly journals Sensitive Immunoassay Detection of Plasmodium Lactate Dehydrogenase by Inductively Coupled Plasma Mass Spectrometry

2021 ◽  
Vol 10 ◽  
Author(s):  
Jianbing Mu ◽  
Lee L. Yu ◽  
Thomas E. Wellems
Keyword(s):  
Mass Spectrometry ◽  
Lactate Dehydrogenase ◽  
Inductively Coupled Plasma ◽  
Sensitive Detection ◽  
Sensor Technology ◽  
Inductively Coupled ◽  
Ms Detection ◽  
Icp Ms ◽  
Highly Sensitive ◽  
Plasma Mass Spectrometry

Rapid, reliable, and sensitive detection of Plasmodium infection is central to malaria control and elimination. Many Malaria Rapid Diagnostic Tests (RDTs) developed for this purpose depend upon immunoassays that can be improved by advances in bound antibody sensor technology. In a previous study, immuno-polymerase chain reaction (PCR) was shown to provide highly sensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH) in monoclonal antibody (mAb) sandwich assays. Here, we show comparably high immunoassay sensitivity by inductively coupled plasma mass spectrometry (ICP-MS) detection of gold nanoparticles (AuNPs). Following capture of PfLDH with the primary mAb and binding of the AuNP-labeled detection mAb, ICP-MS signals from the AuNPs provided quantitative measures of recombinant PfLDH test dilutions and P. falciparum-infected erythrocytes. A detection limit of 1.5 pg/mL was achieved with the PfLDH protein. Parasitemia in cultures of P. falciparum-infected erythrocytes could be detected to a lower limit of 1.6 parasite/μl (p/μl) for early ring-stage forms and 0.3 p/μl for mixed stages including mature trophozoites and schizont-stages. These results show that ICP-MS detection of AuNPs can support highly sensitive and accurate detection of Plasmodium infection.

Single Particle Inductively Coupled Plasma Mass Spectrometry: Investigating Nonlinear Response Observed in Pulse Counting Mode and Extending the Linear Dynamic Range by Compensating for Dead Time Related Count Losses on a Microsecond Timescale

2019 ◽  
Author(s):  
Ingo Strenge ◽  
Carsten Engelhard
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Nonlinear Response ◽  
Dynamic Range ◽  
Dead Time ◽  
Inorganic Nanoparticles ◽  
Linear Dynamic Range ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

<p>The article demonstrates the importance of using a suitable approach to compensate for dead time relate count losses (a certain measurement artefact) whenever short, but potentially strong transient signals are to be analysed using inductively coupled plasma mass spectrometry (ICP-MS). Findings strongly support the theory that inadequate time resolution, and therefore insufficient compensation for these count losses, is one of the main reasons for size underestimation observed when analysing inorganic nanoparticles using ICP-MS, a topic still controversially discussed.</p>

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