New physiologically-relevant liver tissue model based on hierarchically cocultured primary rat hepatocytes with liver endothelial cells

2015 ◽  
Vol 7 (11) ◽  
pp. 1412-1422 ◽  
Author(s):  
Wenjin Xiao ◽  
Guillaume Perry ◽  
Kikuo Komori ◽  
Yasuyuki Sakai
Keyword(s):  
Endothelial Cells ◽  
Liver Tissue ◽  
Human Liver ◽  
Rat Hepatocytes ◽  
Pdms Membrane ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells ◽  
Tissue Model ◽  
Primary Rat Hepatocytes ◽  
Liver Endothelial Cells

We established a physiologically-relevant liver tissue model hierarchically consisting of two complete layers of primary rat hepatocytes and human liver sinusoidal endothelial cells (TMNK-1) on an oxygen-permeable polydimethylsiloxane (PDMS) membrane.

scholarly journals Development of a New Conditionally Immortalized Human Liver Sinusoidal Endothelial Cells

2018 ◽  
Vol 41 (3) ◽  
pp. 440-444 ◽  
Author(s):  
Meiyan Zhu ◽  
Akira Koibuchi ◽  
Hideyuki Ide ◽  
Hanae Morio ◽  
Minaka Shibuya ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Liver ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells

scholarly journals Human liver sinusoidal endothelial cells promote intracellular crawling of lymphocytes during recruitment: A new step in migration

Hepatology ◽  
2016 ◽  
Vol 65 (1) ◽  
pp. 294-309 ◽  
Author(s):  
Daniel A. Patten ◽  
Garrick K. Wilson ◽  
Dalan Bailey ◽  
Robert K. Shaw ◽  
Sirpa Jalkanen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Liver ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells

scholarly journals oxLDL induces injury and defenestration of human liver sinusoidal endothelial cells via LOX1

2014 ◽  
Vol 53 (2) ◽  
pp. 281-293 ◽  
Author(s):  
Qi Zhang ◽  
Jing Liu ◽  
Jia Liu ◽  
Wenhui Huang ◽  
Limin Tian ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Liver ◽  
Underlying Mechanism ◽  
Ros Generation ◽  
Dependent Manner ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells ◽  
Alcoholic Fatty Liver ◽  
Caveolin 1

Non-alcoholic fatty liver disease is associated with hepatic microangiopathy and liver inflammation caused by type 2 diabetes mellitus. Oxidised LDL (oxLDL) is involved in proinflammatory and cytotoxic events in various microcirculatory systems. The lectin-like oxLDL receptor 1 (LOX1) plays a crucial role in oxLDL-induced pathological transformation. However, the underlying mechanism of oxLDL's effects on liver microcirculation disturbances remains unclear. In this study, we investigated the effects of oxLDL on LOX1 (OLR1) expression and function, as well as on the fenestration features of human liver sinusoidal endothelial cells (HLSECs)in vitro. Primary HLSECs were obtained and cultured. The cells were treated with various concentrations of oxLDL (25, 50, 100 and 200 μg/ml), and the cytotoxicity and expression of LOX1 were examined. Furthermore,LOX1knockdown was performed using siRNA technology, and the changes in intracellular reactive oxygen species (ROS), NFκB, p65, (p65), endothelin 1 (ET1 (EDN1)), eNOS (NOS3) and caveolin 1 (CAV1) levels were measured. Cells were treated with 100 μg/ml oxLDL, and the fenestra morphology was visualised using scanning electron microscopy. oxLDL significantly increased LOX1 expression at both the mRNA and protein levels in HLSECs in a dose- and time-dependent manner. oxLDL stimulation increased ROS generation and NFκB activation, upregulated ET1 and caveolin 1 expression, downregulated eNOS expression and reduced the fenestra diameter and porosity. All of these oxLDL-mediated effects were inhibited afterLOX1knockdown. These results reveal a mechanism by which oxLDL stimulates the production of LOX1 through the ROS/NFκB signalling pathway and by which LOX1 mediates oxLDL-induced endothelial injury and the defenestration of HLSECs.

Sign in / Sign up

Export Citation Format

Share Document