Enzyme cascade reactions: synthesis of furandicarboxylic acid (FDCA) and carboxylic acids using oxidases in tandem

2015 ◽  
Vol 17 (6) ◽  
pp. 3271-3275 ◽  
Author(s):  
Shane M. McKenna ◽  
Silke Leimkühler ◽  
Susanne Herter ◽  
Nicholas J. Turner ◽  
Andrew J. Carnell
Keyword(s):  
Carboxylic Acids ◽  
Cascade Reactions ◽  
Preparative Scale ◽  
Mild Conditions ◽  
Enzyme Cascade ◽  
Furandicarboxylic Acid

Three enzymes are combined under mild conditions for the preparative scale oxidation of HMF to FDCA and a range of 10 alcohols.

scholarly journals Mechanically induced solvent-free esterification method at room temperature

RSC Advances ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 5080-5085
Author(s):  
Lei Zheng ◽  
Chen Sun ◽  
Wenhao Xu ◽  
Alexandr V. Dushkin ◽  
Nikolay Polyakov ◽  
...  
Keyword(s):  
Natural Products ◽  
Carboxylic Acids ◽  
Late Stage ◽  
Reaction Mechanisms ◽  
Room Temperature ◽  
Solvent Free ◽  
Mild Conditions

We have developed I2/KH2PO2 and KI/P(OEt)3 strategy syntheses of esters from carboxylic acids and alcohols through different reaction mechanisms. The advantages of present protocol: mild conditions and late-stage diversification of natural products.

Catalytic Synthesis of 2,5‐Furandicarboxylic Acid from Concentrated 2,5‐Diformylfuran Mediated by N ‐hydroxyimides under Mild Conditions

2019 ◽  
Vol 14 (19) ◽  
pp. 3329-3334 ◽  
Author(s):  
Fei Xia ◽  
Jiping Ma ◽  
Xiuquan Jia ◽  
Meiling Guo ◽  
Xuebin Liu ◽  
...  
Keyword(s):  
Catalytic Synthesis ◽  
Mild Conditions ◽  
Furandicarboxylic Acid

scholarly journals Microfluidics Featuring Multilayered Hydrogel Assemblies Enable Real-Time NMR-Monitoring of Enzyme Cascade Reactions

2020 ◽  
Author(s):  
Nurdiana Nordin ◽  
lorenzo bordonali ◽  
Hossein Davoodi ◽  
Novindi Dwi Ratnawati ◽  
Gudrun Gygli ◽  
...  
Keyword(s):  
Real Time ◽  
Functional Performance ◽  
Cascade Reactions ◽  
Inhibitory Effect ◽  
Resonance Spectroscopy ◽  
Reaction Cell ◽  
Enzyme Cascade ◽  
Sensing Applications ◽  
Progress Curve ◽  
Michaelis Constants

Compartmentalized chemical reactions at the microscale are interesting from many perspectives including (multi)functional surfaces and biotechnology. Monitoring the molecular content as a measure of functional performance at these small scales is challenging. As a means to address this challenge, we leverage microtechnology and biocompatible materials to integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resolution nuclear magnetic resonance spectroscopy (NMR). We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi-layered chitosan hydrogel assembly. As a benchmark, we observed the parallel activities of urease (Urs), catalase (Cat), and glucose oxidase (GOx) by recording NMR spectra to extract reagent and product concentrations in real-time. As a result, simultaneous monitoring of a cooperative enzymatic process (GOx + Cat) together with an independent process (Urs) is achieved. Using Michaelis-Menten progress curve analysis of the NMR data, kinetic data is extracted: in the case of GOx, the Michaelis constants (K<sub>M</sub>) are consistent with previous reports, while for Urs, deviations are observed, attributed to an inhibitory effect under our reaction conditions. The system therefore enables the construction of complex reaction cascades with spatial control, as would be interesting in, for example, metabolic engineering and multiplexed sensing applications.

Convenient Preparation of Primary Amides via Activation of Carboxylic Acids with Ethyl Chloroformate and Triethylamine under Mild Conditions

2013 ◽  
Vol 42 (6) ◽  
pp. 580-582 ◽  
Author(s):  
Takuya Noguchi ◽  
Masahiro Sekine ◽  
Yuki Yokoo ◽  
Seunghee Jung ◽  
Nobuyuki Imai
Keyword(s):  
Carboxylic Acids ◽  
Ethyl Chloroformate ◽  
Mild Conditions ◽  
Convenient Preparation

Multi‐enzyme Cascade Reactions in Metal‐organic Frameworks

2020 ◽  
Vol 20 (10) ◽  
pp. 1100-1116 ◽  
Author(s):  
Jieying Liang ◽  
Kang Liang
Keyword(s):  
Metal Organic Frameworks ◽  
Cascade Reactions ◽  
Enzyme Cascade ◽  
Metal Organic

Decarboxylative sulfenylation of amino acids via metallaphotoredox catalysis

2019 ◽  
Vol 6 (18) ◽  
pp. 3224-3227 ◽  
Author(s):  
Lidan Wei ◽  
Chengjuan Wu ◽  
Chen-Ho Tung ◽  
Wenguang Wang ◽  
Zhenghu Xu
Keyword(s):  
Amino Acids ◽  
Visible Light ◽  
Carboxylic Acids ◽  
Mild Conditions ◽  
Cooperative Catalysis

A nickel/visible light photoredox co-catalyzed decarboxylative thiolation reaction of carboxylic acids has been developed. This odorless sulfenylation reaction proceeded well via a nickel/photoredox cooperative catalysis pathway under very mild conditions.

scholarly journals Recent Advances in Enzymatic and Chemoenzymatic Cascade Processes

Catalysts ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1258
Author(s):  
Noelia Losada-Garcia ◽  
Zaida Cabrera ◽  
Paulina Urrutia ◽  
Carla Garcia-Sanz ◽  
Alicia Andreu ◽  
...  
Keyword(s):  
Organic Chemistry ◽  
Solid Phase ◽  
Cascade Reactions ◽  
Review Article ◽  
The Novel ◽  
Enzyme Cascade ◽  
Cascade Processes ◽  
Novel Strategy ◽  
Substantial Interest

Cascade reactions have been described as efficient and universal tools, and are of substantial interest in synthetic organic chemistry. This review article provides an overview of the novel and recent achievements in enzyme cascade processes catalyzed by multi-enzymatic or chemoenzymatic systems. The examples here selected collect the advances related to the application of the sequential use of enzymes in natural or genetically modified combination; second, the important combination of enzymes and metal complex systems, and finally we described the application of biocatalytic biohybrid systems on in situ catalytic solid-phase as a novel strategy. Examples of efficient and interesting enzymatic catalytic cascade processes in organic chemistry, in the production of important industrial products, such as the designing of novel biosensors or bio-chemocatalytic systems for medicinal chemistry application, are discussed

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