High yield production of formate by hydrogenating CO2 derived ammonium carbamate/carbonate at room temperature

2015 ◽  
Vol 17 (5) ◽  
pp. 2769-2773 ◽  
Author(s):  
Ji Su ◽  
Mi Lu ◽  
Hongfei Lin
Keyword(s):  
Room Temperature ◽  
Value Added ◽  
High Yield ◽  
Ammonium Carbamate ◽  
Yield Production ◽  
High Yield Production ◽  
Utilization Strategy ◽  
Hydrogenation Of Co

A new CO2 utilization strategy was developed via hydrogenation of CO2 derived ammonium carbamate/carbonate to produce value-added formate.

scholarly journals Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes

2020 ◽  
Author(s):  
Ching-Lin Hsieh ◽  
Jory A. Goldsmith ◽  
Jeffrey M. Schaub ◽  
Andrea M. DiVenere ◽  
Hung-Che Kuo ◽  
...  
Keyword(s):  
Room Temperature ◽  
Protein A ◽  
Public Health Emergency ◽  
High Yield ◽  
The Novel ◽  
Yield Production ◽  
Novel Coronavirus ◽  
Serological Diagnostics ◽  
High Yield Production

ABSTRACTThe COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has led to accelerated efforts to develop therapeutics, diagnostics, and vaccines to mitigate this public health emergency. A key target of these efforts is the spike (S) protein, a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large quantities. Here, we designed and expressed over 100 structure-guided spike variants based upon a previously determined cryo-EM structure of the prefusion SARS-CoV-2 spike. Biochemical, biophysical and structural characterization of these variants identified numerous individual substitutions that increased protein yields and stability. The best variant, HexaPro, has six beneficial proline substitutions leading to ∼10-fold higher expression than its parental construct and is able to withstand heat stress, storage at room temperature, and multiple freeze-thaws. A 3.2 Å-resolution cryo-EM structure of HexaPro confirmed that it retains the prefusion spike conformation. High-yield production of a stabilized prefusion spike protein will accelerate the development of vaccines and serological diagnostics for SARS-CoV-2.

scholarly journals Structure-based design of prefusion-stabilized SARS-CoV-2 spikes

Science ◽  
2020 ◽  
Vol 369 (6510) ◽  
pp. 1501-1505 ◽  
Author(s):  
Ching-Lin Hsieh ◽  
Jory A. Goldsmith ◽  
Jeffrey M. Schaub ◽  
Andrea M. DiVenere ◽  
Hung-Che Kuo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Room Temperature ◽  
Spike Protein ◽  
High Yield ◽  
Stability Testing ◽  
Freeze Thaw ◽  
Cryo Electron Microscopy ◽  
Yield Production ◽  
Serological Diagnostics ◽  
High Yield Production

The coronavirus disease 2019 (COVID-19) pandemic has led to accelerated efforts to develop therapeutics and vaccines. A key target of these efforts is the spike (S) protein, which is metastable and difficult to produce recombinantly. We characterized 100 structure-guided spike designs and identified 26 individual substitutions that increased protein yields and stability. Testing combinations of beneficial substitutions resulted in the identification of HexaPro, a variant with six beneficial proline substitutions exhibiting higher expression than its parental construct (by a factor of 10) as well as the ability to withstand heat stress, storage at room temperature, and three freeze-thaw cycles. A cryo–electron microscopy structure of HexaPro at a resolution of 3.2 angstroms confirmed that it retains the prefusion spike conformation. High-yield production of a stabilized prefusion spike protein will accelerate the development of vaccines and serological diagnostics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

scholarly journals Review: Managing sheep and goats for sustainable high yield production

animal ◽  
2021 ◽  
pp. 100293
Author(s):  
J. Simões ◽  
J.A. Abecia ◽  
A. Cannas ◽  
J.A. Delgadillo ◽  
D. Lacasta ◽  
...  
Keyword(s):  
High Yield ◽  
Sheep And Goats ◽  
Yield Production ◽  
High Yield Production

scholarly journals High‐Yield Production, Characterization, and Functionalization of Recombinant Magnetosomes in the Synthetic Bacterium Rhodospirillum rubrum “magneticum”

2021 ◽  
pp. 2101017
Author(s):  
Frank Mickoleit ◽  
Sabine Rosenfeldt ◽  
Mauricio Toro‐Nahuelpan ◽  
Miroslava Schaffer ◽  
Anna S. Schenk ◽  
...  
Keyword(s):  
Rhodospirillum Rubrum ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production

High yield production of extracellular recombinant levansucrase by Bacillus megaterium

2012 ◽  
Vol 97 (8) ◽  
pp. 3343-3353 ◽  
Author(s):  
Claudia Korneli ◽  
Rebekka Biedendieck ◽  
Florian David ◽  
Dieter Jahn ◽  
Christoph Wittmann
Keyword(s):  
Bacillus Megaterium ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production

scholarly journals High-yield production of a super-soluble miniature spidroin for biomimetic high-performance materials

2021 ◽  
Author(s):  
Benjamin Schmuck ◽  
Gabriele Greco ◽  
Andreas Barth ◽  
Nicola M. Pugno ◽  
Jan Johansson ◽  
...  
Keyword(s):  
High Performance ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production

A two-phase system for the clean and high yield synthesis of furylmethane derivatives over –SO3H functionalized ionic liquids

2017 ◽  
Vol 19 (20) ◽  
pp. 4804-4810 ◽  
Author(s):  
S. H. Shinde ◽  
C. V. Rode
Keyword(s):  
Ionic Liquids ◽  
Reaction System ◽  
High Yield ◽  
Phase System ◽  
Phase Reaction ◽  
Two Phase ◽  
Yield Production ◽  
High Yield Production

A new and effective unique two-phase reaction system for the high yield production of tri(furyl)methane from furfural and furan.

Unravelling the Mechanisms Behind Mixed Catalysts for the High Yield Production of Single-Walled Carbon Nanotubes

ACS Nano ◽  
2009 ◽  
Vol 3 (12) ◽  
pp. 3839-3844 ◽  
Author(s):  
Sailaja Tetali ◽  
Mujtaba Zaka ◽  
Ronny Schönfelder ◽  
Alicja Bachmatiuk ◽  
Felix Börrnert ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Single Walled Carbon Nanotubes ◽  
High Yield ◽  
Single Walled Carbon ◽  
Yield Production ◽  
Walled Carbon Nanotubes ◽  
High Yield Production
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